Electrochemical Reduction of Diphosphopyridine Nucleotide^*

Electrochemical reduction of NAD+ on a polycrystalline gold electrode

“…In its reduced and enzymatically active form (1,, the molecule transfers two electrons and a proton to a substrate in the presence of a suitable enzyme, resulting in the oxidation of NADH to NAD + . Due to the industrial and biomedical importance of NADH, and also due to its high price, the electrochemical reduction of NAD + to NADH (i.e.…”

“…modified) high-hydrogen-evolution-overpotential electrodes, such as mercury [1][2][3][4][5][6][7][8][9][10] and various carbon materials [11]. It has been shown [2][3][4][5] that the reduction of NAD + on Hg proceeds by the transfer of one electron to the pyridinium ring, but at significantly high cathodic overpotentials (ca.…”

Mechanism of 3,4-dihydroxybenzaldehyde electropolymerization at carbon paste electrodes: catalytic detection of NADH

“…Unfortunately, oxidation of this coenzyme at a bare electrode occurs at high overpotentials and depends on the history of the electrode surface [3][4][5][6] . Electrode poisoning has been also reported 4,5,7,8 and partially solved using the electrochemical pre-treatment procedure described by Blaedel and Jenkins 3,4 . Besides good reproducibility of the results, those authors observed a curvature in log-log current applied potential plots for potentials higher than 0.9 V and small decreases in currents across the transport limited region for replicate scans within a day by using steady-state voltammetry.…”

Submicromolar determination of NADH by means of graphite and glassy carbon electrochemical transducers

“…Direct electrochemical and enzymatic oxidations of NADH have been investigated for industrial quantitative regeneration of NAD' and for developing new enzymatic methods in biochemistry and in clinical laboratories [1][2][3][4][5][6][7][8][9][10].…”

“…It is well known that NAD' is one of the most important coenzymes in many enzyme reactions, and the electrochemical determination of NADH can be correlated to the concentration of substrate according to the following general scheme: cnn nic substrate + NAD' -product + NADH (1) This scheme suggests the possibility of measuring substrates involved in the reaction.…”