Electrochemical Reduction of Metronidazole and Its Determination in Pharmaceutical Dosage Forms by D.C. Polarography

Abstract

A simple d.c. polarographic method has been developed for the determination of metronidazole in dosages forms. In Robinson -Britton buffer (pH 4.38) and in presence of 1.60X10-3% Triton X-100, the drug produced a well defined 4-electron polarographic wave followed by another wave of about half the height of the first wave. The current is proportional to the concentration and permits the drug to be determined by d.c. polarography in the concentration range 5.0X10-5-7.0X10-4M.Results obtained by the proposed method are in excellent agreement with that provided by the USP-XX method. A rapid, sensitive and accurate polarographic method for the determination of metronidazole in the tablets which are produced locally is proposed.

Comparison of the voltammetric behavior of metronidazole at a DNA‐modified glassy carbon electrode, a mercury thin film electrode and a glassy carbon electrode

“…At pH 7. 4 currents for the reduction of metronidazole were observed; however, the linearity was not good and the linear range is only 4.8 to 36.3 pM. The detection limit is 3.6 pM.…”

“…Electrochemical techniques have been applied to the study of the mechanism of action of the nitroimidazoles as antimicrobial agents [3], and in pharmaceutical [4] and clinical [5] analysis. DC polarographic studies [6] showed that the half-wave potential of metronidazole changes in the presence of DNA bases such as adenine, guanine and cytosine, to a lesser extent.…”

A new approach to the spectrophotometric determination of metronidazole and tinidazole using p-dimethylaminobenzaldehyde

“…Several methods have been reported for the determination of metronidazole and/or tinidazole in bulk and dosage forms or in biological fluids. Such methods include non-aqueous titration (4), voltammetric methods (5,6), HPLC (7) and flow injection analysis (8). Some previously described spectrophotometric methods include the use of vanillin and anisaldehyde (9), p-benzoquinone (10), metol and K 2 Cr 2 O 7 (11), alkaline hydrolysis (12), derivative spectrophotometry (13), extractive spectrophotometry using sodium 1,2-naphthaquinone-4-sulfonate after condensation reaction (14) and methylbenzothiazolin-2-one hydrazine (MBTH) (15) among others.…”

Enhanced amperometric detection of metronidazole in drug formulations and urine samples based on chitosan protected tetrasulfonated copper phthalocyanine thin-film modified glassy carbon electrode

“…It is well absorbed and serum concentrations reach high levels, with tissue concentrations being usually like or slightly less than those measured in the serum [15]. Several analytical methods have been reported for determination of MTZ, as well as spectrophotometry [16], polarography [17], liquid chromatography [18,19], gas chromatography [20,21], high performance liquid chromatography [22,23] and gas chromatography-mass spectrometry (GC-MS) [24]. However, the previously mentioned methods do not have sufficient selectivity for MTZ determination.…”