Electrochemical regeneration of nicotinamide adenine dinucleotide

A kinetic study of NAD+ reduction on a ruthenium modified glassy carbon electrode

“…400 mV more negative of our NAD þ reduction peak potential. Also, if we compare independently an NAD þ peak reduction potential and NADH oxidation peak potential reported in [5,23,24], we will see that the reduction-oxidation potential separation is comparable to that observed in Fig. 2.…”

“…We ascribed this peak to the oxidation of NADH formed by the reduction of NAD þ in the negative region at )1.35 V before the polarization scan started. Aizawa et al [23] reported the same positive potential value in their study of oxidation of NADH on a platinum electrode in phosphate buffer pH 7.0. More recently, Katekawa et al [24] studied the oxidation of NADH on a bare glassy carbon electrode using DPV, and reported an NADH oxidation peak potential of ca.…”

Electrochemistry and Electrocatalytic Activity of Pyrocatechol Violet (PCV) Film on a Glassy Carbon Electrode Towards the Oxidation of Reduced Nicotinamide Adenine Dinucleotide(NADH)

“…Although the reversible potential for NADH oxidation is estimated to be À 0.32 V versus normal hydrogen electrode [2], there is typically a very high overpotential, which can be as high as 1.0 V [3]. The electrochemical oxidation of NADH at low overpotentials is of particular interest in biosensor development since about 300 hydrogenases are known whose activities are dependent on the NAD /NADH couple as cofactor.…”

Mechanism of 3,4-dihydroxybenzaldehyde electropolymerization at carbon paste electrodes: catalytic detection of NADH

“…Reviews describing redox and catalytic electrooxidation process of NADH have been published 1,2 . Unfortunately, oxidation of this coenzyme at a bare electrode occurs at high overpotentials and depends on the history of the electrode surface [3][4][5][6] . Electrode poisoning has been also reported 4,5,7,8 and partially solved using the electrochemical pre-treatment procedure described by Blaedel and Jenkins 3,4 .…”

“…Unfortunately, oxidation of this coenzyme at a bare electrode occurs at high overpotentials and depends on the history of the electrode surface [3][4][5][6] . Electrode poisoning has been also reported 4,5,7,8 and partially solved using the electrochemical pre-treatment procedure described by Blaedel and Jenkins 3,4 . Besides good reproducibility of the results, those authors observed a curvature in log-log current applied potential plots for potentials higher than 0.9 V and small decreases in currents across the transport limited region for replicate scans within a day by using steady-state voltammetry.…”