Electrochemical response of an enzyme-loaded polyaniline film

Cooper, Julia C.; Hall, Elizabeth A. H.

doi:10.1016/0956-5663(92)80004-u

Cited by 94 publications

(24 citation statements)

References 24 publications

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“…The redox couple peak at approximately +989 mV corresponds to transition of the PANI from LE to pernigraniline (PE) state, and was accompanied by the oxidation of aniline monomer [43]. The redox couple peak at approximately +541 mV, which was generally attributed to the redox reaction of p-benzoquinone [44]. The cathodic and anodic peak positions of PFO doped PANI shifted and increases the charge transfer with increasing the concentration of the surfactant were shown in the curve from (b-f).…”

Section: Cyclic Voltammogrammentioning

confidence: 99%

Synthesis and Characterization of Perfluorooctanoic Acid Anionic Surfactant Doped Nanosize Polyaniline

Mahalakshmi¹,

Vedhi²

2014

OJSTA

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Surfactant doped polyaniline was synthesized in the aqueous solution of aniline and anionic surfactant of perfluorooctanoic acid (PFO) by chemical synthesis using potassium peroxy disulphate as an oxidant by varying the aniline to surfactant ratio. The solubility of the chemically prepared surfactant doped polyaniline (PANI) was ascertained and it showed good solubility in dimethyl sulfoxide (DMSO), dimethylformamide (DMF), acetone, acetonitrile, ethanol, aceticacid, trichloroethylene, dichloromethane, tetrahydrofuran, ethylacetate, diethylether, toluene, chloroform and sparingly soluble in n-hexane and water. The prepared polymers were characterized by fourier transform infrared spectroscopy (FTIR), UV-visible, X-ray diffraction (XRD), cyclic voltammetric (CV), EIS and scanning electron microscopy (SEM). The analysis of UV-visible and FTIR showed that aniline has been polymerized to PANI in its conducting emeraldine form. FTIR spectra showed that the peaks at 1670, 3315 and 1400 cm −1 corresponded to PFO. FTIR spectra showed that amine peak observed at 1593 cm −1 was shifted to lower wave number due to the interaction between PANI and the surfactant. SEM analysis showed that the variation in morphology of doped PANI was predominantly dependent on the concentration of the surfactant. Elemental analysis was done by energy dispersive spectroscopic (EDAX) which shows the presence of C, N, O, S and F. XRD pattern showed that the formation of nanosized (18 nm) and crystalline polymer. CV studies of the synthesized polymer exhibited good adherent behavior on electrode surface. It exhibited three oxidation peaks at approximately 0.283 V, 0.541 V and 0.989 V and two reduction peaks at 0.1421 and 0.3854 V. Electrical conductivity of PFO doped PANI was studied by impedance spectroscopic method.

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Section: Cyclic Voltammogrammentioning

confidence: 99%

Synthesis and Characterization of Perfluorooctanoic Acid Anionic Surfactant Doped Nanosize Polyaniline

Mahalakshmi¹,

Vedhi²

2014

OJSTA

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“…Film growth was terminated in the potential cycle at ¹0.1 V (vs. SCE), so that the film was in the reduced state and to achieve an estimated charge loading of 0.02 C, to produce the enzyme-loaded polymer (PAGOD) film. It has previously been shown that the glucose oxidase retains activity for about 20 minutes at this low pH [17], which is well within the time scale of the polymerization (ϳ3 minutes).…”

Section: Polyaniline-glucose Oxidasementioning

confidence: 83%

“…All potentials quoted are vs. SCE unless stated otherwise. These films were prepared according to the method of Cooper and Hall [17], [18] by dissolving 3 mgmL ¹1 of glucose oxidase in deionized water and then adding sulfuric acid to achieve a concentration of 0.115 M. Electropolymerization was carried out by cycling between ¹0.1 V and 0.9 V (vs. SCE) at a scan rate of 0.1 Vs ¹1 in a deaerated electrolyte containing 0.115 M sulfuric acid and 0.1 M aniline. Film growth was terminated in the potential cycle at ¹0.1 V (vs. SCE), so that the film was in the reduced state and to achieve an estimated charge loading of 0.02 C, to produce the enzyme-loaded polymer (PAGOD) film.…”

Section: Polyaniline-glucose Oxidasementioning

confidence: 99%

Frequency Domain Selection of the Peroxide Signal for Amperometric Biosensors

et al. 1998

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The amperometric biosensor has become a convenient analytical device concept since the biorecognition element can be highly specific to the analyte of interest when coupled to the appropriate transducer. However, a shortcoming of this system is that the transducer alone is not necessarily specific to the analyte, but only in conjunction with the recognition element. Since many applications require that the sensor be used in complex analytical media, other sample species may cause a direct response at the transducer, for example electroactive species may be directly oxidized or reduced at an electrode in an electrochemical sensor, yielding an erroneous signal. Filtration/membrane barriers have been proposed to overcome this, but this also eliminates any information about the interferent. In this article the role of impedence spectroscopy is examined as a technique to probe overlapping electrochemical signals by separation in the frequency domain. Hydrogen peroxide is examined as a model to test the feasibility of extraction of quantitative data in this control mode, and experimental conditions are determined where analytical data may be obtained to extract the peroxide signal from other electrochemical information in an enzyme biosensor based on peroxide determination.

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“…15,16 The glucose sensor is affected by: (i) polyaniline conductivity, (ii) GOD activity in the polymeric film, and (iii) oxygen permeability through the polymeric film. These properties are influenced by many experimental factors, such as pH values of the polymerization solution, the measuring medium, enzyme loading and film thickness.…”

Section: Polymerization Of Aniline and Immobilization Of Glucose Oxidmentioning

confidence: 99%

An Enzyme-Chemically Modified Carbon Paste Electrode as a Glucose Sensor Based on Glucose Oxidase Immobilized in a Polyaniline Film

Luo

Cui

1999

ANAL. SCI.

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Electrochemical response of an enzyme-loaded polyaniline film

Cited by 94 publications

References 24 publications

Synthesis and Characterization of Perfluorooctanoic Acid Anionic Surfactant Doped Nanosize Polyaniline

Synthesis and Characterization of Perfluorooctanoic Acid Anionic Surfactant Doped Nanosize Polyaniline

Frequency Domain Selection of the Peroxide Signal for Amperometric Biosensors

An Enzyme-Chemically Modified Carbon Paste Electrode as a Glucose Sensor Based on Glucose Oxidase Immobilized in a Polyaniline Film

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