Electrochemical sensing of guanine, adenine and 8-hydroxy-2′-deoxyguanosine at glassy carbon modified with single-walled carbon nanotubes covalently functionalized with lysine

Gutiérrez, Alejandro García; Gutiérrez, Fabiana; Eguílaz, Marcos; González‐Domínguez, José M.; Hernández‐Ferrer, Javier; Ansón‐Casaos, Alejandro; Martínez, María Teresa; Rivas, Gustavo A.

doi:10.1039/c5ra22556f

Cited by 31 publications

(7 citation statements)

References 72 publications

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“…Such devices establish a direct reading of 8-OHdG, making use of its active redox properties on glassy carbon (Gutiérrez et al, 2016) (Jia et al, 2015)(L. Jia et al, 2013)(L. Yang et al, 2015) or pyrolytic graphitic (Gupta et al, 2016) supports, modified with highly conductive nanomaterials of multi-wall carbon nanotubes or graphene. In general, the detection capability of these devices varies from 1.1 to 97 nM, but some methods have shown severe interference from urine elements (mostly uric acid), solved therein by the addition of specific enzyme prior to the analysis stage.…”

Section: Various Methods Have Been Proposed To Analyze 8-ohdg In Biolmentioning

confidence: 99%

8-hydroxy-2′-deoxyguanosine (8-OHdG) biomarker detection down to picoMolar level on a plastic antibody film

Martins

Marques

Fortunato

et al. 2016

Biosensors and Bioelectronics

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Section: Various Methods Have Been Proposed To Analyze 8-ohdg In Biolmentioning

confidence: 99%

8-hydroxy-2′-deoxyguanosine (8-OHdG) biomarker detection down to picoMolar level on a plastic antibody film

Martins

Marques

Fortunato

et al. 2016

Biosensors and Bioelectronics

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“…In another study, synthesized single-walled carbon nanotubes were covalently modified with L-lysine and used for high efficient electrochemical measurement of guanine, adenine and 8-hydroxy-20-deoxyguanosine. 25 Multiwalled carbon nanotubes with chemical functional groups on the surface are efficiently used to measure the DNA of Escherichia coli (E. coli) microorganism by wrapping DNA molecules. 26 The DNA of E.coli was measured in 20 min with measurement limit of 0.5 pmol in 30 μL samples.…”

Section: Introductionmentioning

confidence: 99%

Lawsone assisted preparation of carbon nanofibers for the selective detection of miRNA molecules

Sahtani

Aykut

Tanik

2021

J of Chemical Tech & Biotech

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BACKGROUND: microRNA (miRNA) molecules are considered as biomarkers and have promising future for early-stage cancer diagnosis. Lawsone (Law) enriched carbon nanofibers (CNFs) were prepared via electrospinning process for the selective miRNA detection. Anti-miRNA molecules were attached on the CNFs immobilized screen printed electrodes (SPE) and used for the detection of miRNA molecules by observing guanine oxidation signal via differential pulse voltammetry (DPV) method. Three different miRNA molecules including the target (miRNA), single-base mismatched (SM.miRNA) and non-complementary (NC. miRNA) were hybridized with the previously attached anti-miRNA molecules (with guanine [PolyT(G)] and without guanine [PolyT(I)]) on the CNFs immobilized SPE surfaces.RESULTS: Guanine oxidation signal decreased after the hybridization of miRNA with anti-miRNA molecules on CNF immobilized SPE surfaces. Guanine oxidation signal was not detected on the PolyT(I) attached samples, but the existence of guanine oxidation signal was clear detected after the addition of miRNA molecules on the previously attached PolyT(I). Because no hybridization of anti-miRNA molecules with the non-complementary miRNA (NC.miRNA) molecules occurred, the guanine oxidation signal was not significantly decreased after the interaction of NC.miRNA with the attached PolyT(G). Since there was no hybridization between the NC.miRNA molecules with the attached PolyT(I) on the SPEs, weak guanine peaks were detected at PolyT(I)-NC.miRNA samples as a result of the existence of residual NC.miRNA molecules on SPE after washing. CONCLUSIONS: The measurement results confirm the enhanced selectivity of the miRNA molecules by using CNFs with SPEs and the developed biosensory system could be used to detect the specific RNA molecules.

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“…Carbon nanotubes (CNTs) show remarkable electrical conductivity and specific surface area. Processed into CNT films, they reveal great promise as electrode materials for various applications such as resistive gas sensors [1][2][3] or electrochemical sensors, achieving high sensitivity and selectivity for the detection of inorganic cations [4,5] and organic molecules [6][7][8][9]. Besides, conducting electrodes and interfaces made of CNTs or containing CNT additives have been developed for electrochemical energy harvesting and storage devices [10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Carbon Nanotube Film Electrodes with Acrylic Additives: Blocking Electrochemical Charge Transfer Reactions

et al. 2020

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Carbon nanotubes (CNTs) processed into conductive films by liquid phase deposition technologies reveal increasing interest as electrode components in electrochemical device platforms for sensing and energy storage applications. In this work we show that the addition of acrylic latex to water-based CNT inks not only favors the fabrication of stable and robust flexible electrodes on plastic substrates but, moreover, sensitively enables the control of their electrical and electrochemical transport properties. Importantly, within a given concentration range, the acrylic additive in the films, being used as working electrodes, effectively blocks undesired faradaic transfer reactions across the electrode–electrolyte interface while maintaining their capacitance response as probed in a three-electrode electrochemical device configuration. Our results suggest a valuable strategy to enhance the chemical stability of CNT film electrodes and to suppress non-specific parasitic electrochemical reactions of relevance to electroanalytical and energy storage applications.

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Electrochemical sensing of guanine, adenine and 8-hydroxy-2′-deoxyguanosine at glassy carbon modified with single-walled carbon nanotubes covalently functionalized with lysine

Abstract: Synthesis and characterization of l-lysine covalently functionalized SWCNT and analytical application for the highly sensitive quantification of guanine, adenine and 8-hydroxy-2′-deoxyguanosine.

Cited by 31 publications

References 72 publications

8-hydroxy-2′-deoxyguanosine (8-OHdG) biomarker detection down to picoMolar level on a plastic antibody film

8-hydroxy-2′-deoxyguanosine (8-OHdG) biomarker detection down to picoMolar level on a plastic antibody film

Lawsone assisted preparation of carbon nanofibers for the selective detection of miRNA molecules

Carbon Nanotube Film Electrodes with Acrylic Additives: Blocking Electrochemical Charge Transfer Reactions

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