Electrochemical sensing of trypanosome- and flavivirus-related neglected tropical diseases

Lima, Dhésmon; Hacke, Ana Carolina Mendes; Ulmer, Benjamin; Kuss, Sabine

doi:10.1016/j.coelec.2021.100838

Cited by 9 publications

(4 citation statements)

References 91 publications

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“…Correct NS1 certifies a DENV infection; at the same time, incorrect outcomes do not get rid of infection possibility. IgM‐based test is then performed if incorrect outcome occurs 58 . After 7 days, SN1 is not recommended, which is its major limitations.…”

Section: Conventional Methodsmentioning

confidence: 99%

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Innovations in dengue virus detection: An overview of conventional and electrochemical biosensor approaches

Raza,

Poria,

Kala

et al. 2024

Biotech and App Biochem

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Globally, people are in great threat due to the highly spreading of viral infectious diseases. Every year like 100–300 million cases of infections are found, and among them, above 80% are not recognized and irrelevant. Dengue virus (DENV) is an arbovirus infection that currently infects people most frequently. DENV encompasses four viral serotypes, and they each express comparable sign. From a mild febrile sickness to a potentially fatal dengue hemorrhagic fever, dengue can induce a variety of symptoms. Presently, the globe is being challenged by the untimely identification of dengue infection. Therefore, this review summarizes advances in the detection of dengue from conventional methods (nucleic acid‐based, polymerase chain reaction‐based, and serological approaches) to novel biosensors. This work illustrates an extensive study of the current designs and fabrication approaches involved in the formation of electrochemical biosensors for untimely identifications of dengue. Additionally, in electrochemical sensing of DENV, we skimmed through significances of biorecognition molecules like lectins, nucleic acid, and antibodies. The introduction of emerging techniques such as the CRISPR/Cas’ system and their integration with biosensing platforms has also been summarized. Furthermore, the review revealed the importance of electrochemical approach compared with traditional diagnostic methods.

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Section: Conventional Methodsmentioning

confidence: 99%

“…IgMbased test is then performed if incorrect outcome occurs. 58 After 7 days, SN1 is not recommended, which is its major limitations.…”

Section: Ns1-based Testsmentioning

confidence: 99%

Innovations in dengue virus detection: An overview of conventional and electrochemical biosensor approaches

Raza,

Poria,

Kala

et al. 2024

Biotech and App Biochem

View full text Add to dashboard Cite

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“…Electroanalytical methods have proven to be sensitive, affordable, and ecofriendly alternatives to provide accurate determinations of analytes in samples of variable complexity. For instance, these techniques have been applied to detect pharmaceuticals, vitamins, proteins, and biological molecules qualitatively and quantitatively with limits of detection reaching the nanomolar range. − …”

Section: Introductionmentioning

confidence: 99%

Electrifying Fruit Juice: Integrating Applied Electroanalytical Chemistry into the Undergraduate Curriculum

Lima,

Singh,

Bulleeraz

et al. 2024

J. Chem. Educ.

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Electroanalytical chemistry has been advanced through portable devices, providing methods and sensors for the detection of analytes with high sensitivity and accuracy. This subfield of electrochemistry has the potential to be utilized in industry and analytical quality control, in general. This results in an increasing demand for trained personnel, capable of operating benchtop and portable electroanalytical equipment. Although electrochemical techniques are routinely taught in theoretical undergraduate courses, they need to be more often incorporated into experimental didactic activities. Herein, we describe the application of an effective, hands-on, and low-maintenance experiment that can enhance the learning experience of electroanalytical methods and their use in industrial quality control settings. This activity is based on the detection of ascorbic acid (vitamin C) by employing cyclic voltammetry at unmodified glassy carbon electrodes (GCE) in real juice samples. This didactic experiment teaches students about the theoretical concepts of cyclic voltammetry, three-electrode cell setup, chemical reversibility, data treatment, and quantitative analysis. This teaching approach was conducted in a second-year analytical chemistry course and was easily adapted to social distancing measures imposed by the COVID-19 pandemic.

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“…Considering the high costs and long cycles associated with new drug development, greater efforts must be made to develop new therapeutic strategies for optimal control of trypanosomiasis [17]. Recently, the application of nanotechnology offers new opportunities for the control of trypanosomiasis, involving drug delivery systems, therapeutic agents [18], diagnostic materials [19] and vaccine adjuvants [20], etc. Various nanomaterials, including polymers [21], lipids [22], and metallic/inorganic systems [18] have demonstrated their utility in the diagnosis and treatment of protozoan diseases including trypanosomiasis.…”

Section: Introductionmentioning

confidence: 99%

Graphene quantum dots harvest anti-trypanosomatid efficacy by disrupting antioxidant networks centered on trypanothione reductase

Zhang

Jü

et al. 2023

Preprint

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Background Trypanosomiasis is a highly lethal infectious disease caused by trypanosome, leading to a severe social and economic burden worldwide. Due to the lack of mechanism research, application of the promising nanomaterials and nanotechnologies in treatment of trypanosomiasis is limited. Results Herein, the toxicological effects induced by graphene quantum dots (GQDs) on T. brucei and the underlying mechanism are investigated. First, the biological/cytotoxic effects are evaluated, including endotytosis, cell viability, apoptosis, ROS production and morphological defects of subcellular organelles. Considering the few experimentally-determined 3D structures of T. brucei proteins, next, a computed structure database of T. brucei genome-wide proteins is constructed from I-TASSER, AlphaFold2 and MD simulation. Then, the database is used for docking with GQDs, and two goups of potential target proteins with transporter activity and antioxidant activity are screened out. Last, TryR stands out as a vital target due to its high binding energy with GQDs at active site and its key role in the trypanothione-dependent antioxidant network of T. brucei, which is further verified by theoretical (MD simulation) and experimental (BLI, inhibition of enzyme activity) means. Conclusions Evidences from this study suggest that GQD-induced cytotoxicity on T. brucei results from interference of GQDs with the lineage-specific antioxidant network with TryR as a key target. These findings provide theoretical insights into the rational design of nanomedical materials for trypanosomiasis.

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Electrochemical sensing of trypanosome- and flavivirus-related neglected tropical diseases

Cited by 9 publications

References 91 publications

Innovations in dengue virus detection: An overview of conventional and electrochemical biosensor approaches

Innovations in dengue virus detection: An overview of conventional and electrochemical biosensor approaches

Electrifying Fruit Juice: Integrating Applied Electroanalytical Chemistry into the Undergraduate Curriculum

Graphene quantum dots harvest anti-trypanosomatid efficacy by disrupting antioxidant networks centered on trypanothione reductase

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