Electrochemical sensor based on molecularly imprinted copolymer for selective and simultaneous determination of Ascorbic acid and Tyrosine

Abstract

This study reported a novel electrochemical sensor based on molecularly imprinted polymer (MIP) for simultaneous and selective detection of Ascorbic acid (AA) and Tyrosine (Tyr). The MIP film was electropolymerized on the glassy carbon electrode (GCE) using of o-aminophenol (o-AP) and m-dihydroxy benzene (m-DB) as monomers and the dual analyte of AA and Tyr, and its electrochemical performance was evaluated. Influencing parameters such as the pH value, electropolymerization cycle numbers, and template/monomer ratio were optimized. Differential pulse voltammetry (DPV) technique was used for the simultaneous and individual determination of AA and Tyr in their binary mixture. The introduced sensor showed the linear concentration ranges of 0.1–300 µM for AA and 0.01–180 µM for Tyr and good limits of detection were 0.03 µM and 0.003 µM, respectively. The proposed sensor was successfully employed to detect AA and Tyr in real samples.

Green Synthesis of a Molecularly Imprinted Polymer Based on a Novel Thiophene-Derivative for Electrochemical Sensing

“…In this regard, electrochemical sensors meet this requirement by assuring a rapid, selective and reproducible response to the aminoacid target, as well as the possibility to specifically functionalize the electrode surface and to miniaturize the device itself, allowing an affordable and early diagnosis of patient conditions [51][52][53][54]. For example, Karazan and Roushani achieved the simultaneous detection of tyrosine and ascorbic acid by a glassy carbon electrode functionalized with a dual molecularly imprinted polymer based on o-aminophenol and m-dihydroxy benzene, showing limit of detection values in the nanomolar range, fast and selective sensor responses and adequate recovery estimations in spiked serum sample [55]. Mahdi et al easily developed an electrochemical sensor based on molecularly imprinted catechol and para-aminophenol for the concomitant detection of tryptophan, dopamine and riboflavin, reporting a linear and selective response towards the analytes with low limit of detection values [56].…”