Electrochemical sensor for the assessment of carbohydrate deficient transferrin: Application to diagnosis of congenital disorders of glycosilation

Sierra, Tania; Crevillén, Agustín G.; Escarpa, Alberto

doi:10.1016/j.bios.2021.113098

Cited by 9 publications

(10 citation statements)

References 21 publications

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“…Application to diagnosis of CDG I in serum samples. As it was previously demonstrated, [51] the greater the amount of glycans in Tf, the higher the number of Os (VI) complex molecules bound to Tf. This allowed us to propose a new approach to measure the level of CDT, based on the ratio generated by the relation between Os (VI) signal and protein signal (EIG).…”

Section: Resultssupporting

confidence: 58%

“…Since 3 independent devices were used in each analysis, these results revealed the excellent inter-device reproducibility even during the analysis of clinical samples (RSD < 9 %, n = 3). In addition, these results were compared with those obtained by our previous reported method 51 (see Table S1) and there were not significant differences (test-t, P = 0.05, two tailed). Figure 3 shows control serum and CDG type I sample voltamperograms.…”

Section: Resultsmentioning

confidence: 89%

“…It was prepared according to the bibliography. [51,52] The proposed microfluidic device must not only perform the labeling of Tf with Os(VI) complex inside the main channel, but also it must carry out the electrochemical detection of TfÀ Os(VI) adduct. Firstly, the main channel placed on the 5 th layer was filled with water (30 μL was added to the upper left reservoir) (Figure 1A a).…”

Section: Resultsmentioning

confidence: 99%

“…For this reason, 60 min was selected as the best incubation time and used in the following experiments. In our previous work, [51] the labeling of transferrin with Os(VI) complex to carry out the EIG measurements involved a time of 16 hours, besides this step was not integrated into the analytical device. These results reveal the analytical potency of microfluidics where not only the reactions are integrated but also, they are favored by the high surface to volume ratio.…”

Section: Resultsmentioning

confidence: 99%

“…In this sense, our group have recently proposed a new parameter to measure the glycosylation level of Tf, called "electrochemical index of glycosylation (EIG)". [51] This parameter showed an excellent correlation with the official parameter % CDT and it can be easily measured by a simple screen-printed electrode. In addition, this methodology was able to differentiate between serum from CDG patients and healthy individuals.…”

Section: Introductionmentioning

confidence: 83%

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Disposable Passive Electrochemical Microfluidic Device for Diagnosis of Congenital Disorders of Glycosylation

et al. 2021

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A disposable pump-free electrochemical microfluidic device made by using a multilayer lamination technique is proposed for diagnosis of glycosylation disorders. Thanks to the stacking of polyethylene terephthalate (PET) films and double adhesive layers, it is possible to create a geometry that allows the filling of the tailored channels in a fixed time. In the main channel of this passive microfluidic device, the necessary steps to perform the electrochemical determination of transferrin are performed: labeling of the protein with Os (VI) complex (electrochemical tag), washing with water of the osmium residues and electro-chemical detection by adsorptive transfer stripping square wave voltammetry. Electrochemical detection released two electrochemical signals: one from Os (VI) complex due to carbohydrates at À 0.9 V, and other from the intrinsic electrochemical signal of glycoprotein due to the amino acids at + 0.8 V. The ratio between them establishes an indicator of the degree of glycosylation (called electrochemical index of glycosylation). The method was successfully applied to the analysis of clinical samples from patients with congenital disorders of glycosylation.

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Section: Resultssupporting

confidence: 58%

Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 83%

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