Electrochemical strategy for pyrophosphatase detection Based on the peroxidase-like activity of G-quadruplex-Cu2+ DNAzyme

Wang, Yonghong; Wu, Yaohui; Liu, Wen; Chu, Lei; Liao, Zhibin; Guo, Wentao; Liu, Gao-Qiang; He, Xiaoxiao; Wang, Kemin

doi:10.1016/j.talanta.2017.09.069

Cited by 25 publications

(9 citation statements)

References 39 publications

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“…Besides hemin, it was demonstrated that the human telomeric DNA assembled with Cu 2+ ions can present DNAzyme activity, being able to catalyze the Friedel-Crafts reaction in water with excellent enantioselectivity [ 134 , 135 ]. Based on a Cu 2+ /GQ DNAzyme, an electrochemical method for pyrophosphatase (PPase) activity detection was developed [ 136 ]. In the absence of PPase, Cu 2+ coordinated with pyrophosphate (PPi) to form a Cu 2+ -PPi compound.…”

Section: G-quadruplex Electrochemical Biosensor Applicationsmentioning

confidence: 99%

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Electrochemical and AFM Characterization of G-Quadruplex Electrochemical Biosensors and Applications

Chiorcea-Paquim

Eritja

Oliveira‐Brett

2018

Journal of Nucleic Acids

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Guanine-rich DNA sequences are able to form G-quadruplexes, being involved in important biological processes and representing smart self-assembling nanomaterials that are increasingly used in DNA nanotechnology and biosensor technology. G-quadruplex electrochemical biosensors have received particular attention, since the electrochemical response is particularly sensitive to the DNA structural changes from single-stranded, double-stranded, or hairpin into a G-quadruplex configuration. Furthermore, the development of an increased number of G-quadruplex aptamers that combine the G-quadruplex stiffness and self-assembling versatility with the aptamer high specificity of binding to a variety of molecular targets allowed the construction of biosensors with increased selectivity and sensitivity. This review discusses the recent advances on the electrochemical characterization, design, and applications of G-quadruplex electrochemical biosensors in the evaluation of metal ions, G-quadruplex ligands, and other small organic molecules, proteins, and cells. The electrochemical and atomic force microscopy characterization of G-quadruplexes is presented. The incubation time and cations concentration dependence in controlling the G-quadruplex folding, stability, and nanostructures formation at carbon electrodes are discussed. Different G-quadruplex electrochemical biosensors design strategies, based on the DNA folding into a G-quadruplex, the use of G-quadruplex aptamers, or the use of hemin/G-quadruplex DNAzymes, are revisited.

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Section: G-quadruplex Electrochemical Biosensor Applicationsmentioning

confidence: 99%

“…Using 3,3′,5,5′-tetramethylbenzidine as a redox mediator, the Cu 2+ /GQ DNAzyme catalyzed the reduction of H 2 O 2 to generate a quantitative chronoamperometric signal. This method was additionally applied to screen the sodium fluoride inhibitor for PPase [ 136 ].…”

Section: G-quadruplex Electrochemical Biosensor Applicationsmentioning

confidence: 99%

Electrochemical and AFM Characterization of G-Quadruplex Electrochemical Biosensors and Applications

Chiorcea-Paquim

Eritja

Oliveira‐Brett

2018

Journal of Nucleic Acids

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“…A variety of analytical methods toward UA detection have thus far been reported, including colorimetry [9], fluorometry [10], high‐performance liquid chromatography [11], electrophoresis [12] and electrochemistry [13–15]. Conventionally, enzyme‐based electrochemical methods take unparalleled advantages due to their specificity [16, 17]; however, most enzymes are unstable under conditions such as acid, alkali, and heat, and their structural changes cause them to lose their catalytic activity [18, 19]. In addition, the content of natural enzymes is shallow, and it is difficult to obtain a large amount by purification, so the price is high, which greatly limits its practical application [20].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Enhanced Detection of Uric Acid Based on Peroxidase‐like Activity of Fe₃O₄@Au

et al. 2021

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Fe3O4@Au nanomaterials were prepared by the self‐assembly method. An enzyme‐free, ultrasensitive electrochemical detection of uric acid was achieved based on the peroxidase‐like activity of Fe3O4@Au. The proposed procedure has exhibited excellent catalytic activities and achieved significant enhancements of the current responses to uric acid. The detection range was from 0.1 to 10 mmol/L, and the limit of detection was 0.087 μmol/L. Under the action of external magnetic field, the magnetic particles can be easily separated from the bottom liquid, which has the advantages of simple operation and high separation efficiency. Moreover, this detection method combining a simulated enzyme and electrochemical can enhance the effective output of the overall electrochemical signal without modifying the electrode, and excellent reproducibility can be achieved. Compared to colorimetric assay, the electrochemical one has higher sensitivity and selectivity, and was further applied in ultrasensitive detection of uric acid in food samples. In short, the proposed electrochemical assay has great potential in the fields of food quality control and biomedical analysis.

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“…So far, several analytical techniques have been developed for PPase detection. Wang et al used a G-quadruplex modified screen-printed electrode for PPase detection [ 4 ]. A two-stage reaction of H 2 O 2 –TMB was carried out upon the release of Cu 2+ from the PPi complex after hydrolysis by PPase.…”

Section: Introductionmentioning

confidence: 99%

DNAzyme-Amplified Label-Free Biosensor for the Simple and Sensitive Detection of Pyrophosphatase

et al. 2021

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The level of pyrophosphatase (PPase) expression has been suggested as a potential biomarker of various cancers, and its prognostic value has been evaluated in patients suffering from lung cancer, colorectal cancer, and hyperthyroidism. However, the detection of PPase usually needs specific materials that require complicated, time-consuming reactions with restricted linear range and sensitivity, limiting their application in early clinical diagnosis. Herein, we developed a DNAzyme-based biosensor for the detection of PPase. In the presence of PPase, pyrophosphate (PPi) and Cu2+ ions released from the PPi–Cu2+–PPi complex induce the cleavage of the DNAzyme and the corresponding substrate. An apurinic/apyrimidinic (AP) site was elaborately designed within substrates that could encase the fluorophore 2-amino-5,6,7-trimethyl-1,8-naphthyridine (ATMND). The fluorescence of ATMND was initially quenched but restored when the DNAzyme/substrate complex was hydrolyzed with the release of ATMND. In this way, the PPase activity can be estimated by detecting the increased fluorescence of the released ATMND. Under optimized conditions, the activity of PPase could be analyzed at concentrations from 0.5 to 1000 mU, with the lowest detectable concentration being 0.5 mU. This work lays a foundation for developing a DNAzyme-amplified fluorescent biosensor with a high sensitivity, a wide linear range, and single-step operation for use as an easy diagnostic for PPase analysis.

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Electrochemical strategy for pyrophosphatase detection Based on the peroxidase-like activity of G-quadruplex-Cu2+ DNAzyme

Cited by 25 publications

References 39 publications

Electrochemical and AFM Characterization of G-Quadruplex Electrochemical Biosensors and Applications

Electrochemical and AFM Characterization of G-Quadruplex Electrochemical Biosensors and Applications

Electrochemical Enhanced Detection of Uric Acid Based on Peroxidase‐like Activity of Fe₃O₄@Au

DNAzyme-Amplified Label-Free Biosensor for the Simple and Sensitive Detection of Pyrophosphatase

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Electrochemical strategy for pyrophosphatase detection Based on the peroxidase-like activity of G-quadruplex-Cu2+ DNAzyme

Cited by 25 publications

References 39 publications

Electrochemical and AFM Characterization of G-Quadruplex Electrochemical Biosensors and Applications

Electrochemical and AFM Characterization of G-Quadruplex Electrochemical Biosensors and Applications

Electrochemical Enhanced Detection of Uric Acid Based on Peroxidase‐like Activity of Fe3O4@Au

DNAzyme-Amplified Label-Free Biosensor for the Simple and Sensitive Detection of Pyrophosphatase

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Electrochemical Enhanced Detection of Uric Acid Based on Peroxidase‐like Activity of Fe₃O₄@Au