Electrochemical study of S–nitrosoglutathione and nitric oxide by carbon fibre NO sensor and cyclic voltammetry – possible way of monitoring of nitric oxide

Víteček, Jan; Petrlová, Jitka; Petřek, Jiřı́; Adam, Vojtěch; Potěšil, David; Havel, Ladislav; Mikelová, Radka; Trnková, Libuše; Kizek, René

doi:10.1016/j.electacta.2006.03.079

Cited by 36 publications

(16 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many techniques, approaches, and procedures have been proposed for investigation of GSH complexes with other molecules [4,12,[14][15][16][17]. Electrochemical methods seem to be very advantageous for the study of GSH [4,18,19].…”

Section: Introductionmentioning

confidence: 99%

“…Reduced glutathione plays a number of key roles in the control of signal processes, cell division, immune response, programmed cell death, and detoxification of some xenobiotics and heavy metals [1,2]. From a physicochemical point of view, GSH is highly reactive and is often found conjugated to other molecules via its sulfhydryl moiety, including heavy metal ions [3,4].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Complexes of glutathione with heavy metal ions as a new biochemical marker of aquatic environment pollution

Baloun

Adam

Trnková

et al. 2009

Environmental Toxicology and Chemistry

Self Cite

View full text Add to dashboard Cite

Reduced glutathione (GSH) plays a number of key roles in many biochemical pathways. This peptide is highly reactive and forms conjugates with other molecules via its sulfhydryl moiety. The interactions of the common heavy metal pollutant Cd(II) with GSH were determined by using the Brdicka reaction to evaluate whether this technique would be suitable as a biomarker. After GSH interaction with Cd(II) ions, two characteristic changes in the measured voltammogram were observed: Cat2 signal height decreased, and a new signal called P1 was found. The observed signal probably relates to the formation of a GSH-heavy metal ion complex adsorbed on the surface of the working electrode. When the interaction of GSH with cisplatin was studied, the same characteristic changes in the voltammogram were observed, which confirmed our hypothesis. Moreover, changes in the height of P1 and Cat2 signals with increasing time of GSH interaction with Cd(II) ions and/or cisplatin were also investigated. Cat2 peak height decreased proportionally with increasing time of interaction. This decrease can be explained by shielding of free sulfhydryl moiety by heavy metal ions, so it cannot catalyze the evolution of hydrogen from the supporting electrolyte. In addition, we found that, with increasing time of the interaction, the P1 signal was enhanced and shifted to more positive potentials for both Cd(II) ions and cisplatin.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Complexes of glutathione with heavy metal ions as a new biochemical marker of aquatic environment pollution

Baloun

Adam

Trnková

et al. 2009

Environmental Toxicology and Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…This is due to a redox activity of GSNO. Indeed, similar reductive peak was recently reported in the case of GSNO on a hanging mercury electrode [18]. But the most remarkable result is that no redox process occurs in the potential range where GSH is expected to be oxidized.…”

mentioning

confidence: 72%

Cobalt Phthalocyanine Molecular Electrode for the Electrochemical Investigation of the Release of Glutathione upon Copper‐Catalyzed Decomposition of S‐Nitrosoglutathione

et al. 2007

View full text Add to dashboard Cite

Decomposition of S-nitrosoglutathione (GSNO) in phosphate buffer solution at physiological pH 7.4 in the presence of cuprous ion as a catalyst and sodium borohydride as a reducing agent is analyzed by observing the transient apparition of reduced glutathione GSH through its electrooxidation. Transient formation of GSH, upon decomposition of 1 mM GSNO in presence of 0.025 mM Cu(NO 3 ) 2 and 1 mM NaBH 4 was detected by using an ordinary pyrolytic graphite electrode modified with an adsorbed monolayer of cobalt phthalocyanine at 0 V vs. SCE.

show abstract

“…This increases the informative capacity of the assay and eliminates interference between species. For example, with scanning voltammetry, the concentrations of all redox species in a sample, including NO, NO 2 − and N x O y or GSH, GSSG and GSNO, can be determined by the distribution and amplitude of anodic current peaks [325,326].…”

Section: Electrochemical Detection (Ecd)-electrochemical Detection (Ementioning

confidence: 99%

“…A variety of amperometric sensors specifically developed for the analysis of NO recently has been reviewed [325,326,328,329]. In combination with CE, the ECD techniques (CEECD) expand the range of accessible analytes without derivatization.…”

Section: Electrochemical Detection (Ecd)-electrochemical Detection (Ementioning

confidence: 99%

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

Boudko

2007

Journal of Chromatography B

View full text Add to dashboard Cite

This review briefly summarizes recent progress in fundamental understanding and analytical profiling of the L-arginine/nitric oxide (NO) pathway. It focuses on key analytical references of NO actions and on the experimental acquisition of these references in vivo, with capillary electrophoresis (CE) and high-performance capillary electrophoresis (HPCE) comprising one of the most flexible and technologically promising analytical platform for comprehensive high-resolution profiling of NO-related metabolites. Second aim of this review is to express demands and bridge efforts of experimental biologists, medical professionals and chemical analysis-oriented scientists who strive to understand evolution and physiological roles of NO and to develop analytical methods for use in biology and medicine.

show abstract

Electrochemical study of S–nitrosoglutathione and nitric oxide by carbon fibre NO sensor and cyclic voltammetry – possible way of monitoring of nitric oxide

Cited by 36 publications

References 85 publications

Complexes of glutathione with heavy metal ions as a new biochemical marker of aquatic environment pollution

Complexes of glutathione with heavy metal ions as a new biochemical marker of aquatic environment pollution

Cobalt Phthalocyanine Molecular Electrode for the Electrochemical Investigation of the Release of Glutathione upon Copper‐Catalyzed Decomposition of S‐Nitrosoglutathione

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

Contact Info

Product

Resources

About