Electrochemical study of the interaction between cytochrome P450sccK201E and cholesterol

Catabolism and biotechnological applications of cholesterol degrading bacteria

“…Several authors have also shown that the P450scc cytochrome, an isoform of the P450 (CYP) cytochrome superfamily, which catalyses the side‐chain cleavage of cholesterol to pregnenolone in the presence of molecular oxygen and NADPH‐ferrihaemoprotein reductase (Carrara et al ., 2008), can be utilized for determination of cholesterol with high sensitivity (Antonini et al ., 2004; Paternolli et al ., 2004; Shumyantseva et al ., 2004; 2005). The highly specific conversion of steroid hormones by cythocromes makes feasible a specific substrate determination in complex media by using CYP based sensors (Bistolasa et al ., 2005)…”

Electrochemical reduction of cytochrome P450 as an approach to the construction of biosensors and bioreactors

“…Riboflavin-graphite screen-printed electrodes were employed as mediators directly immobilised on the surface of working electrodes. Cytochrome P450scc native recombinant (P450sccNR) and K201E P450scc mutant in solution were studied for the enzyme-substrate interaction using electrochemical methods [70]. Screen-printed electrodes were used as working electrodes.…”

Cholesterol biosensor based on cholesterol esterase, cholesterol oxidase and peroxidase immobilized onto conducting polyaniline films

“…The detection limit and the sensitivity of the agarose based electrode were 155 M and 6.9 nA M −1 with a linearity coefficient of 0.99, whereas the sensitivity and lower detection limit of the biosensor based on the immobilization procedure of flavocytochrome P450scc by glutaraldehyde, were 13.8 nA M −1 and 300 M with a linearity coefficient of 0.98 for cholesterol using sodium cholate as a detergent. Antonini et al [26] reported the electrochemical study of the interaction between cholesterol and cytochrome P450sccK201E. The estimation of cholesterol was accomplished by an electrochemical method using cyclic voltammetry and chronoamperometry, and the results showed that the engineered enzyme electrode could be utilized for the cholesterol detection.…”