Electrochemical study of Type 304 and 316L stainless steels in simulated body fluids and cell cultures

Tang, Yee Chin; Katsuma, Shoji; Fujimoto, Shinji; Hiromoto, Sachiko

doi:10.1016/j.actbio.2006.06.003

Cited by 128 publications

(67 citation statements)

References 11 publications

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“…16) They reported that the proliferation of cells cultured on nonporous aluminum oxide with diameters of 30 nm (the smallest diameter examined in their work) was higher than that of cells cultured on at surfaces and nanoporous aluminum oxides with other diameters (40,45, and 50 nm). Schmuki et al also studied the adhesion, spreading, growth, and differentiation of mesenchymal stem cells on nanotubular titanium oxides with various diameters (15,20,30,50,70, and 100 nm) and reported that nanotubes with diameters less than 30 nm, particularly those with a diameter of 15 nm, were most preferable. 17,18) Thus, our current ndings coincide with those of previous studies obtained for different substrates, despite the distinct differences in substrate compositions, e.g., aluminum, titanium, and stainless steels.…”

Section: Cell Morphologymentioning

confidence: 99%

“…The samples were further modi ed with the following surface nishes: #2000 polished, mirror-nished, or nanopores formed at 20, 30, and 40 V. The electrochemical process used to form nanopores was carried out in ethylene glycol containing 2.0 M NaClO 4 using a two-electrode electrochemical cell with a platinum counter electrode. The sample was potentiodynamically polarized from 0 V to desired voltages (20,30, and 40 V) with a scan rate of 0.1 V/s and then kept at the voltage for 300 s. After the electrochemical process, the samples were ultrasonically cleaned in acetone and ethanol, rinsed with deionized water, and dried. The surface of the samples was characterized using a eld-emission scanning electron microscope (FE-SEM; JSM-6500; JEOL Ltd.) and an atomic force microscope (AFM; SPM-9500; Shimadzu Co.).…”

Section: Sample Preparationmentioning

confidence: 99%

“…Thus, we have performed a series of studies on stainless steels as biomaterials, [30][31][32][33] investigating their corrosion behaviors, and surface modi cations for improvement of osseoconductivity. Such surface modi cations have the potential to promote the application of stainless steels as implant materials.…”

Section: Introductionmentioning

confidence: 99%

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Cell Activity on Type 316L Stainless Steel with Self-Organized Nanopores Formed by Anodic Polarization

et al. 2016

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In this study, we evaluated the osseoconductivity of Type 316L stainless steel with self-organized nanopores of three different average diameters (26, 90, and 177 nm), formed by anodic polarization. The proliferation, alkaline phosphatase activity, and morphology of MC3T3-E1 mouse osteoblast-like cells, cultured on the self-organized nanopores were evaluated. The cell densities on samples with the nanopores were higher than those on mechanically nished surfaces that were mirror-polished or ground with #2000 SiC paper. In particular, the highest cell density and alkaline phosphatase activity were obtained on the nanoporous sample with the smallest diameter of 26 nm. Cells on the samples with 26 nm nanopores extended further and spread more lopodia compared with cells on samples with the other surface morphologies. Therefore, we concluded that self-organized nanopores with an optimal diameter (e.g., 26 nm) on Type 316L stainless steel could enhance long-term cell activity.

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Section: Cell Morphologymentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

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Cell Activity on Type 316L Stainless Steel with Self-Organized Nanopores Formed by Anodic Polarization

et al. 2016

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“…It has been well used in biomedical areas typically as surgical implant [1], cardiac peacemaker electrodes [2], dental fixation as plates or screws [3] and orthodontic archwires or brackets [4] due to its well-behaved corrosion resistance, excellent mechanical performance, favorable bio-affinity, as well as good machinability and low cost. Although traditional microcrystalline 304ss implants provide better biomechanical properties than other biomedical metals such as cobalt chromium alloys, titanium [5], localized corrosion of microcrystalline 304ss implants like pitting or stress corrosion were recently reported within the environment of the human body [6,7]. Even in particular cases, some metallic ions from traditional microcrystalline 304ss dissolved out and lead toxic or side effects around [8].…”

Section: Introductionmentioning

confidence: 99%

<i>In Vitro</i> Corrosion and Haemocompatibility of Bulk Nanocrystalline 304 Stainless Steel by Severe Rolling

Nie

Wang

Shi

et al. 2010

MSF

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Abstract. Bulk nanocrystalline 304 stainless steel (nanocrystalline 304ss) discs had been successfully prepared by the commercial microcrystalline 304 stainless steel (microcrystalline 304ss) plate using severe rolling technique. Micro-hardness was measured to reveal the different mechanical behavior after the severe plastic deformation. The electrochemical corrosion resistance and ion release behavior after immersion of the samples were investigated in Hank's solution for its potential use as implant and orthodontic appliance in body. Furthermore, murine fibroblast cells were indirectly employed to detect cytotoxicity by co-incubation with the extraction from the given materials. Haemocompatibility, consisting of hemolysis test and adhesion of the platelets, was also measured with fresh human whole blood and platelet-rich plasma respectively. Polarization resistance trials indicate that nanocrystalline 304ss is more corrosion resistant in the Hank's solution, with lower current density and superficial corrosion morphologies. The release values of the biotoxic ions after immersion do not exceed the set limit and turn to be well below the critical value necessary to induce allergy and below daily dietary intake level. Cellular interaction is observed via the proliferated feature of the cell line. Hemolysis and platelet adhesion results elucidates that nanocrystalline 304ss is biological and hematologic compatible.

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“…So far, a variety of Fe-based systems such as Fe-Mn, Fe-Pd and Fe-Mn-Si [1][2][3][4][5][6] has been or are being developed for the potential applications. The degradability of Fe-based DBMs can avoid various complications, such as chronic inflammation, thrombosis, in-stent restenosis [7,8] or even a second surgery to remove the implants [9,10], compared to the corrosion-resistant implants such as stainless steels 316L, Ti-based and Co-Cr-based alloys [11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Effect of Immersion in Simulated Body Fluid on the Mechanical Properties and Biocompatibility of Sintered Fe–Mn-Based Alloys

Hodgson

Cao

2016

Metals

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Fe-Mn-based degradable biomaterials (DBMs) are promising candidates for temporary implants such as cardiovascular stents and bone fixation devices. Identifying their mechanical properties and biocompatibility is essential to determine the feasibility of Fe-Mn-based alloys as DBMs. This study presents the tensile properties of two powder metallurgical processed Fe-Mn-based alloys (Fe-28Mn and Fe-28Mn-3Si, in mass percent) as a function of immersion time in simulated body fluid (SBF). In addition, short-term cytotoxicity testing was performed to evaluate the in vitro biocompatibility of the sintered Fe-Mn-based alloys. The results reveal that an increase in immersion duration deteriorated the tensile properties of both the binary and ternary alloys. The tensile properties of the immersed alloys were severely degraded after being soaked in SBF for ≥45 days. The ion concentration in SBF released from the Fe-28Mn-3Si samples was higher than their Fe-28Mn counterparts after 7 days immersion. The preliminary cytotoxicity testing based on the immersed SBF medium after 7 days immersion suggested that both the Fe-28Mn-3Si and Fe-28Mn alloys presented a good biocompatibility in Murine fibroblast cells.

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Electrochemical study of Type 304 and 316L stainless steels in simulated body fluids and cell cultures

Cited by 128 publications

References 11 publications

Cell Activity on Type 316L Stainless Steel with Self-Organized Nanopores Formed by Anodic Polarization

Cell Activity on Type 316L Stainless Steel with Self-Organized Nanopores Formed by Anodic Polarization

<i>In Vitro</i> Corrosion and Haemocompatibility of Bulk Nanocrystalline 304 Stainless Steel by Severe Rolling

Effect of Immersion in Simulated Body Fluid on the Mechanical Properties and Biocompatibility of Sintered Fe–Mn-Based Alloys

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