Electrochemical Therapy to Treat Cancer (In Vivo Treatment)

Telló, Marcos; Oliveira, Luciana Oliveira de; Parise, Orlando; Buzaid, Antônio C.; Oliveira, R.T.; Zanella, Rodrigo; Cardona, Andrés Felipe

doi:10.1109/iembs.2007.4353091

Cited by 12 publications

(8 citation statements)

References 3 publications

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“…In vivo studies using electrochemical methods as potential therapeutics for diseases such as cancer have been reported in the literature. [14][15][16] In the present study, electrochemical initiation of CO-release from the complex was confirmed and quantified via headspace analysis using gas chromatography (ESI, † Fig. S6).…”

mentioning

confidence: 57%

Controlled CO release using photochemical, thermal and electrochemical approaches from the amino carbene complex [(CO)₅CrC(NC₄H₈)CH₃]

McMahon

Rochford

Halpin

et al. 2014

Phys. Chem. Chem. Phys.

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Multimodal photo, thermal and electrochemical approaches toward CO release from the amino carbene complex [(CO) 5 CrC(NC 4 H 8 )CH 3 ] is reported. Picosecond time resolved infrared spectroscopy was used to probe the photo-induced early state dynamics leading to CO release, and DFT calculations confirmed that CO release occurs from a singlet excited state.CO releasing molecules (CORMs) are an area of current interest due to the physiological role of CO in the body. 1,2 CO is endogenously generated during oxidative heme degradation by the heme oxygenase (HO) enzymes. 3 The potential therapeutic applications of CO include vasodilation, anti-inflammatory and anti-proliferative effects. CORMs have been developed to deliver CO in a controlled manner. However, there is a major challenge in controlled CO release, be it photochemically, thermally or enzymatically induced. It is well known that chromium carbene complexes can release CO both thermally and photochemically, as the resulting tetracarbonyl reactive intermediates have been used as synthons in organic chemistry. 4 Recently, Lynam et al. reported the suitability of heteroatom (amino, methoxy and thio) stabilised chromium carbenes as thermal CORMs. 5 Rapid CO release was observed for the thio-and methoxy carbenes (t 1/2 = 313 s and 306 s, respectively, for a 60 mM solution of the complex to achieve an Mb-CO concentration of 30 mM), compared to slow release in the case of the amino analogue which required more than 2 h for a 60 mM solution of the complex to form 10 mM of carboxy-myoglobin (Mb-CO). In addition the chromium carbonyls investigated, demonstrated the fastest and most regulated CO release characteristics compared to the molybdenum or tungsten complexes studied. 5 In this communication we set out to compare the rate of CO release from [(CO) 5 CrC(NC 4 H 8 )CH 3 ] using photochemical, thermal and electrochemical stimulation. To the best of our knowledge this is first example of a CORM involving electrochemical stimulation. In the present study we report our findings on a ps-time resolved infrared (ps-TRIR) study on [(CO) 5 CrC(NC 4 H 8 )CH 3 ] in conjunction with DFT calculations to investigate the excited state responsible for the CO release. Chromium Fischer carbenes have received significant attention as photochemical synthons for a range of organic compounds. Photolysis of [(CO) 5 CrC(XR)R 0 ] in the presence of imines, olefins, aldehydes, or alcohols are known to yield b-lactams, cyclobutanones, b-lactones or amino esters respectively. 6 The proposed mechanism for these reactions involves a metal-toligand charge transfer photoexcitation resulting in the formation of a ketene intermediate which is formed by the insertion of a cis-CO ligand into the adjacent Cr-carbene bond (Fig. 1). Experimental and theoretical calculations reported by Sierra et al. support the proposal that this occurs through a MLCT triplet excited state. 7 Although a number of low temperature and time-resolved studies have investigated photo-induced CO release from chromium carbene...

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mentioning

confidence: 57%

Controlled CO release using photochemical, thermal and electrochemical approaches from the amino carbene complex [(CO)₅CrC(NC₄H₈)CH₃]

McMahon

Rochford

Halpin

et al. 2014

Phys. Chem. Chem. Phys.

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“…Some clinical studies have reported the ability of electrolysis to destroy tumor tissue in various organs such as the skin, liver, or breast. However, complete regression of a malignancy was rarely observed [Berry et al, 2000;Telló et al, 2007]. EChT-like, high-intensity focused ultrasound or RFA is not selective and will destroy normal cells as well as tumor cells.…”

Section: Electrochemical Treatment Of Cancermentioning

confidence: 96%

Microsecond and nanosecond electric pulses in cancer treatments

Breton¹,

Mir²

2011

Bioelectromagnetics

184

133

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New local treatments based on electromagnetic fields have been developed as non-surgical and minimally invasive treatments of tumors. In particular, short electric pulses can induce important non-thermal changes in cell physiology, especially the permeabilization of the cell membrane. The aim of this review is to summarize the present data on the electroporation-based techniques: electrochemotherapy (ECT), nanosecond pulsed electric fields (nsPEFs), and irreversible electroporation (IRE). ECT is a safe, easy, and efficient technique for the treatment of solid tumors that uses cell-permeabilizing electrical pulses to enhance the activity of a non-permeant (bleomycin) or low permeant (cisplatin) anticancer drug with a very high intrinsic cytotoxicity. The most interesting feature of ECT is its unique ability to selectively kill tumor cells without harming normal surrounding tissue. ECT is already used widely in the clinics in Europe. nsPEFs could represent a drug free, purely electrical cancer therapy. They allow the inhibition of tumor growth, and interestingly, nsPEF can target intracellular organelles. However, many questions remain on the mechanism of action of these pulses. Finally, IRE is a new ablation procedure using pulses that provoke the permanent permeabilization of the cells resulting in their death. This technique does not result in any thermal effect, which is its main advantage in current physical ablation technologies. For both the nsPEF and the IRE, the preservation of the normal tissue, which is characteristic of ECT, has not yet been shown and their safety and efficacy still have to be investigated thoroughly in vivo and in the clinics.

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“…The electropermeabilization or electroporation of the cells in the tumours induces an electrically mediated reorganization of the plasma membrane allowing the chemotherapeutic to be absorbed via passive diffusion. ECT can be used to treat cutaneous and subcutaneous tumours (e.g., sarcomas, carcinomas, melanoma and mastocytoma) regardless of histological type …”

Section: Introductionmentioning

confidence: 99%

The treatment of canine mast cell tumours with electrochemotherapy with or without surgical excision

Lowe

Gavazza

Impellizeri

et al. 2016

Vet Comparative Oncology

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To describe the results of electrochemotherapy (ECT) in dogs with mast cell tumours (MCTs) either as first line therapy or as an adjuvant to surgery. The treatment combines administration of low dose chemotherapeutic drugs with the application of microsecond electric pulses, which cause the temporary permeabilization and increased porosity of the tumour cell membranes. The design of this study is a retrospective case series. A total of 51 dogs with MCTs were included and classified according to ECT procedure into 4 groups (ECT only, 15 cases, intra-surgery ECT, 11, ECT Adjuvant to surgery, 14, Surgery followed by ECT, 11). The four groups (staged with location, size and grade) were evaluated to assess complete or partial remission, disease free interval, overall survival time and local toxicity. In this case series, Boxers, mixed breed and Labrador Retrievers, male dogs, between 4 and 9 years old were more represented. MCTs were predominantly grade 2 (Patnaik) and T stage 0-1, I-1 (World Health Organization). Treated lesions were most commonly identified on the hindlimb and head where curative surgery would involve cosmetic or functional compromise. The intra-surgery group of dogs showed the best disease free interval with Kaplan-Meyer analysis. Local toxicity induced by ECT ranged mostly from 1 to 4 in a 5-point arbitrary scale with 0 - no toxicity to 5 - highest toxicity. In this study, ECT can be applied successfully as an exclusive therapy in smaller MCTs as an alternative to surgery. ECT can be combined with surgery either intra-operatively or post operatively for larger lesions without significant toxicity.

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Electrochemical Therapy to Treat Cancer (In Vivo Treatment)

Cited by 12 publications

References 3 publications

Controlled CO release using photochemical, thermal and electrochemical approaches from the amino carbene complex [(CO)₅CrC(NC₄H₈)CH₃]

Controlled CO release using photochemical, thermal and electrochemical approaches from the amino carbene complex [(CO)₅CrC(NC₄H₈)CH₃]

Microsecond and nanosecond electric pulses in cancer treatments

The treatment of canine mast cell tumours with electrochemotherapy with or without surgical excision

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Electrochemical Therapy to Treat Cancer (In Vivo Treatment)

Cited by 12 publications

References 3 publications

Controlled CO release using photochemical, thermal and electrochemical approaches from the amino carbene complex [(CO)5CrC(NC4H8)CH3]

Controlled CO release using photochemical, thermal and electrochemical approaches from the amino carbene complex [(CO)5CrC(NC4H8)CH3]

Microsecond and nanosecond electric pulses in cancer treatments

The treatment of canine mast cell tumours with electrochemotherapy with or without surgical excision

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Product

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Controlled CO release using photochemical, thermal and electrochemical approaches from the amino carbene complex [(CO)₅CrC(NC₄H₈)CH₃]

Controlled CO release using photochemical, thermal and electrochemical approaches from the amino carbene complex [(CO)₅CrC(NC₄H₈)CH₃]