Electrochemically controlled detection of adrenaline on poly(2‐aminobenzylamine) thin films by surface plasmon resonance spectroscopy and quartz crystal microbalance

Abstract

In this study, we present an electrochemically controlled surface plasmon resonance (EC‐SPR) biosensor to detect adrenaline on poly(2‐aminobenzylamine) (P2ABA) thin films. The P2ABA thin films are stable and display electroactivity in a neutral PBS solution. Specific detection of adrenaline was performed on P2ABA thin films because the benzylamine groups in the P2ABA structure could specifically react with adrenalines. Adrenaline was detected in real time by EC‐SPR spectroscopy, which provides an EC‐SPR reflectivity change on the P2ABA thin film upon adrenaline injection. The measured responses were quite different from those for uric acid and ascorbic acid, which are major interferences in adrenaline detection. The electrochemically applied potential facilitates the specific detection of adrenaline. In addition, the detection of adrenaline on the P2ABA thin films was investigated by a quartz crystal microbalance technique. The detection limit for adrenaline at open circuit potential was 10 pM. The present study provides a useful information on the detection of adrenaline on the P2ABA thin films. Copyright © 2013 John Wiley & Sons, Ltd.

Polyanilines with Pendant Amino Groups as Electrochemically Active Copolymers at Neutral pH

“…As mentioned in the Introduction, the electroactivity and stability of the homopolymer of ABA is not clear from previous studies . To clarify this point, we first studied the voltammetric synthesis of the homopolymer of ABA.…”

“…Furthermore, its incorporation was not possible beyond a few voltammetric cycles, indicating that the electropolymerization of the 2‐ABA homopolymer does not proceed to a large extent . However, more recently, the polymerization of both 2‐ABA and 3‐aminobenzylamine (ABA) in acid solution has been reported to produce stable films that can then be employed for adrenaline and uric acid sensing. Also, 4‐aminobenzylamine has recently been shown to electropolymerize on graphene in LiClO 4 aqueous solution .…”

Effect of silica coating and further silica surface decoration by phospholipid bilayer on quenching of Tb(III) complexes by adrenochrome

“…It is also worth noting that the presence of the bowl-like calix [4]arene and thiacalix [4]arene cavities (Scheme 1c) is the prerequisite for the inclusion complex formation with organic molecules [37,40,41]. The lack of any detectable effect of the catecholamines on the luminescence of [Tb(TCAS)] in buffer solution According to literature data the formation of AD from EP is slow at pH 6, while this process is greatly accelerated under pH increase and complex formation with metal ions [25][26][27]. As it has been abovementioned the EP → AD transformation manifests itself by the appearance of the absorption band at 480 nm ( Fig.…”

“…Unfortunately this approach is not selective enough to discriminate between EP and DA. The selective binding of definite catecholamine with a surface should be noted as rather promising approach to discriminate one from another [21,[25][26][27][28][29]. Nevertheless most of the literature data highlight the EP versus DA selectivity as complicated and unresolved problem [20,30].…”

A sensitive electrochemical immunosensor based on poly(2-aminobenzylamine) film modified screen-printed carbon electrode for label-free detection of human immunoglobulin G

“…In particular, polyaniline, one of most important conducting polymers has emerged as an intriguing material because of their specific redox properties, low cost, ease of production and good electrical conductivity along with their excellent biocompatibility [22,23]. In this work, poly(2-aminobenzylamine), a polyaniline derivative, was selected for electrode fabrication in the proposed immunosensor, because 1) poly(2-aminobenzylamine) provides selective detection of biomolecules as reported in the previous article using surface plasmon resonance [24] and 2) poly(2-aminobenzylamine) is easy to manipulate in terms of the deposition and formation rate of the polymer during electropolymerization [24]. In addition, the amino group (-NH 2 ) in the poly(2-aminobenzylamine) structure acts as if it was a chemical scaffold for antibody to immobilize through amide bond on poly(2-aminobenzylamine) thin film.…”