Electrochemically deposited nanocomposite film of CS-Fc/Au NPs/GOx for glucose biosensor application

Qiu, Jian‐Ding; Wang, Rui; Liang, Ru‐Ping; Xia, Xing‐Hua

doi:10.1016/j.bios.2009.02.029

Cited by 87 publications

(39 citation statements)

References 60 publications

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“…As presented in Figure 4c, the amperometric response increased from pH 4.5 to 7.4 to reach the maximum, and then decreased over pH 7.4. It is well known that the bioactivity of the immobilized protein and the stability of CS are solution pH dependent [40,41]. Alkaline solution will decrease the protein activity.…”

Section: Optimization Of Analytical Conditionsmentioning

confidence: 99%

A Label‐Free Amperometric Immunosensor Based on Redox‐Active Ferrocene‐Branched Chitosan/Multiwalled Carbon Nanotubes Conductive Composite and Gold Nanoparticles

et al. 2010

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A novel immunosensor has been developed by self-assembling Au NPs onto a ferrocene-branched chitosan/multiwalled carbon nanotubes (CS-Fc/MWCNTs) modified electrode for the sensitive determination of hepatitis B surface antigen (HBsAg). The formation of CS-Fc effectively avoids the leakage of Fc and retains its electrochemical activity. Incorporation of MWCNTs and Au NPs into CS-Fc further increases the electrochemical active Fc in the CS films and provides interactive sites for the immobilization of HBsAb. The morphologies and electrochemistry of the formed biofilm were investigated by using scanning electron microscopy and electrochemical techniques. The immunosensor exhibits a specific response to HBsAg in the range of 1.0-420 ng mL À1. Excellent analytical performance, fabrication reproducibility and operational stability of the proposed immunosensor indicated its promising application in clinical diagnostics.

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Section: Optimization Of Analytical Conditionsmentioning

confidence: 99%

A Label‐Free Amperometric Immunosensor Based on Redox‐Active Ferrocene‐Branched Chitosan/Multiwalled Carbon Nanotubes Conductive Composite and Gold Nanoparticles

et al. 2010

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“…Porém, as suas aplicações não se resumem apenas a aplicações industriais. A GOx é a enzima mais bem estudada para a atuação em biossensores de glicose devido a sua alta especificidade, estabilidade e baixo custo de obtenção quando comparada a outras enzimas [28][29][30]. Neste caso, a GOx pura deve ser imobilizada na superfície do eletrodo, de modo que se mantenha presa a este suporte, mantendo sua atividade catalítica.…”

Section: Introductionunclassified

Avaliação da formação do híbrido NiFe2O4@SiO2 e sua performance na imobilização da GOx

et al. 2018

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RESUMORecentes avanços tecnológicos na síntese e projeto de nanopartículas magnéticas (NPMs) vêm abrindo vários caminhos para o uso destes materiais em aplicações médicas e farmacêuticas, como biosensores, carreadores de fármacos, destruição de células tumorais e separação magnética de células e proteínas. Com vista nisso, foi proposto obter o híbrido de NiFe 2 O 4 @SiO 2 a partir da modificação da superfície da ferrita de níquel com 3-aminopropiltrietoxissilano, objetivando avaliar a sua formação a partir de suas propriedades estruturais, magnéticas e morfológicas, a fim de estudar a sua capacidade de imobilizar a glicose oxidase (GOx). As amostras foram analisadas por DRX, FTIR, MEV, BET/BJH, medidas magnéticas e testadas na reação de imobilização da GOx. Os resultados revelaram a formação monofásica do espinélio NiFe 2 O 4 com tamanho de cristalito de 35 e 32 nm para as amostras antes e após a modificação da superfície; presença de bandas de absorção características do espinélio e do grupo silanol do agente silano, confirmando formação do híbrido. A modificação de superfície melhorou a área superficial e o comportamento magnético das NPMs. O estudo demonstrou que a presença do agente silano não contribuiu para a imobilização da GOx.Palavras-Chave: Ferritas de níquel, nanopartículas magnéticas, agente silano, imobilização, biomaterial. ABSTRACTRecent technological advances in the synthesis and design of magnetic nanoparticles (MNPs) have opened up a slew of ways for the use of these materials in medical and pharmaceutical applications, such as biosensors, drug carriers, destruction of tumor cells, and magnetic separation of cells and proteins. In view of this, we propose to obtain the hybrid NiFe 2 O 4 @SiO 2 from the surface modification of the nickel ferrite with 3-aminopropyltriethoxysilane, aiming to evaluate its formation by its structural, morphological and magnetic properties, in order to test it for its performance in the immobilization of the glucose oxidase (GOx). The samples were analyzed through XRD, FTIR, SEM, BET/BJH, magnetic measurements and tested in the GOx immobilization. The results showed the single phase formation of the spinel NiFe 2 O 4 with crystallite size of 35 and 32 nm for the samples before and after surface modification and the presence of characteristic absorption bands for the spinel and the silanol group of the silane agent, confirming the formation of the hybrid. The surface modification improved the surface area and the magnetic behavior of the NPMs. The presence of the silane agent does not contribute for the GOx immobilization.

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“…Various materials have been used for the immobilization of enzymes in order to fabricate excellent biosensors [4][5][6][7]. The main studies are focused on the development of new materials with good electron transfer capability, nice biocompatibility, good stability, easy accessibility towards the analyte and large surface area for immobilization of enzymes [8][9][10]. However it is difficult to find a single material which can include all these important characteristics.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Bienzymatic Glucose Biosensor Based on Novel Gold Nanoparticles‐Bacteria Cellulose Nanofibers Nanocomposite

Wang

Zhang

et al. 2010

Electroanalysis

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An exploration of gold nanoparticles-bacterial cellulose nanofibers (Au-BC) nanocomposite as a platform for amperometric determination of glucose is presented. Two enzymes, glucose oxidase (GOx) and horseradish peroxidase (HRP) were immobilized in Au-BC nanocomposite modified glassy carbon electrode at the same time. A sensitive and fast amperometric response to glucose was observed in the presence of electron mediator (HQ). Both of GOx and HRP kept their biocatalytic activities very well in Au-BC nanocomposite. The detection limit for glucose in optimized conditions was as low as 2.3 mM with a linear range from 10 mM to 400 mM. The biosensor was successfully applied to the determination of glucose in human blood samples.

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Electrochemically deposited nanocomposite film of CS-Fc/Au NPs/GOx for glucose biosensor application

Cited by 87 publications

References 60 publications

A Label‐Free Amperometric Immunosensor Based on Redox‐Active Ferrocene‐Branched Chitosan/Multiwalled Carbon Nanotubes Conductive Composite and Gold Nanoparticles

A Label‐Free Amperometric Immunosensor Based on Redox‐Active Ferrocene‐Branched Chitosan/Multiwalled Carbon Nanotubes Conductive Composite and Gold Nanoparticles

Avaliação da formação do híbrido NiFe2O4@SiO2 e sua performance na imobilização da GOx

Fabrication of Bienzymatic Glucose Biosensor Based on Novel Gold Nanoparticles‐Bacteria Cellulose Nanofibers Nanocomposite

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