Abstract:Graphene oxide (GO) exhibits good mechanical and physicochemical characteristics and has extensive application prospects in bone tissue engineering. However, its effect on angiogenesis is unclear, and its potential toxic effects are heavily disputed. Herein, we found that nanographene oxide (NGO) synthesized by one-step water electrolytic oxidation is smaller and shows superior biocompatibility. Moreover, NGO significantly enhanced angiogenesis in calvarial bone defect areas in vivo, providing a good microenvi… Show more
“…Moreover, it was also suggested that eNOS/NO signaling pathway might be one of the causes of the pro-angiogenesis effect. According to the current studies, it was believed that the angiogenic effect of GO was mostly confined to its delivery of relative growth factors, such as CD31, CD34, VEGF, MMP9, and SDF-1, and it had been also shown that GO could activate endothelial tip cells and promote angiogenesis by binding endogenous lysophosphatidic acid 45 , which may be another reason for better pro-angiogenesis effect in the 1.0 wt% GO/PLLA group compared with 0.0 wt% GO/PLLA group.…”
In this study, we successfully constructed the new graphene oxide/poly-
L
-lactic acid (GO/PLLA) nanofiber scaffolds with a hydrophilic surface and porous network structure that were highly favorable for cell infiltration. When employed these new nanofiber scaffolds for a wide range of tissue engineering applications, it was expected to promote graft tissue survival and angiogenesis. The new GO/PLLA nanofiber scaffold with an appropriate concentration of 1.0 wt% was applied for the restoration of ovarian function and reserve in mice with primary ovarian insufficiency (POI). After co-transplanting the normal ovarian cortex loaded on these new nanomaterials into the in situ ovarian tissue of POI mice, the fusion of transplanted ovarian cortex with damaged ovarian tissue was improved, as well as the ovarian function and the follicle numbers. Moreover, angiogenesis was observed clearly and proved to exist in the transplanted tissue and nanomaterials, with the most conspicuous effect after co-transplantation with 1.0 wt% GO/PLLA nanofiber scaffold. In addition, nitric oxide (NO) production by phosphorylated endothelial nitric oxide synthase (p-eNOS) in vivo was proven to be involved in the effect of GO and PLLA on the improved survival rate of the transplanted ovarian cortex. This study provides a new method for the fertility preservation of ovarian tissue cryopreservation and transplantation, as well as a new strategy for the transplantation of other organs.
“…Moreover, it was also suggested that eNOS/NO signaling pathway might be one of the causes of the pro-angiogenesis effect. According to the current studies, it was believed that the angiogenic effect of GO was mostly confined to its delivery of relative growth factors, such as CD31, CD34, VEGF, MMP9, and SDF-1, and it had been also shown that GO could activate endothelial tip cells and promote angiogenesis by binding endogenous lysophosphatidic acid 45 , which may be another reason for better pro-angiogenesis effect in the 1.0 wt% GO/PLLA group compared with 0.0 wt% GO/PLLA group.…”
In this study, we successfully constructed the new graphene oxide/poly-
L
-lactic acid (GO/PLLA) nanofiber scaffolds with a hydrophilic surface and porous network structure that were highly favorable for cell infiltration. When employed these new nanofiber scaffolds for a wide range of tissue engineering applications, it was expected to promote graft tissue survival and angiogenesis. The new GO/PLLA nanofiber scaffold with an appropriate concentration of 1.0 wt% was applied for the restoration of ovarian function and reserve in mice with primary ovarian insufficiency (POI). After co-transplanting the normal ovarian cortex loaded on these new nanomaterials into the in situ ovarian tissue of POI mice, the fusion of transplanted ovarian cortex with damaged ovarian tissue was improved, as well as the ovarian function and the follicle numbers. Moreover, angiogenesis was observed clearly and proved to exist in the transplanted tissue and nanomaterials, with the most conspicuous effect after co-transplantation with 1.0 wt% GO/PLLA nanofiber scaffold. In addition, nitric oxide (NO) production by phosphorylated endothelial nitric oxide synthase (p-eNOS) in vivo was proven to be involved in the effect of GO and PLLA on the improved survival rate of the transplanted ovarian cortex. This study provides a new method for the fertility preservation of ovarian tissue cryopreservation and transplantation, as well as a new strategy for the transplantation of other organs.
“…After further addition of pDA/nHA, the BV/TV value of PP-pDA/nHA-LPA increased by 17.1% to PP-LPA, signifying that the addition of nHA could improve the osteogenesis effect of LPA, and the two could synergistically promote osteogenesis in vivo . In addition, some studies have shown that LPA2 and LPA6 signaling could promote angiogenesis and maintain vascular homeostasis, which is a key part of tissue repair. , This synergistic effect was possibly due to the stable LPA accelerating the migration of osteoblasts while superimposing the properties of HA to promote the proliferation and differentiation of osteoblasts.…”
Section: Resultsmentioning
confidence: 99%
“…In addition, some studies have shown that LPA2 and LPA6 signaling could promote angiogenesis and maintain vascular homeostasis, which is a key part of tissue repair. 64,65 This synergistic effect was possibly due to the stable LPA accelerating the migration of osteoblasts while superimposing the properties of HA to promote the proliferation and differentiation of osteoblasts.…”
Guided
bone regeneration (GBR) technology has been widely
used
for the regeneration of periodontal bone defects. However, the limited
mechanical properties and bone regeneration potential of the currently
available GBR membranes often limit their repair effectiveness. In
this paper, serum-derived growth factor lysophosphatidic acid (LPA)
nanoparticles and dopamine-decorative nanohydroxyapatite (pDA/nHA)
particles were double-loaded into polylactic-glycolic acid/polycaprolactone
(PLGA/PCL) scaffolds as an organic/inorganic biphase delivery system,
namely, PP-pDA/nHA-LPA scaffolds. Physicochemical properties and osteogenic
ability in vitro and in vivo were
performed. Scanning electron microscopy and mechanical tests showed
that the PP-pDA/nHA-LPA scaffolds had a 3D bionic scaffold structure
with improved mechanical properties. In vitro cell
experiments demonstrated that the PP-pDA/nHA-LPA scaffolds could significantly
enhance the attachment, proliferation, osteogenic differentiation,
and mineralization of MC3T3-E1 cells. In vivo, the
PP-pDA/nHA-LPA scaffolds exhibited great cytocompatibility and cell
recruitment ability in 2- and 4-week subcutaneous implantation experiments
and significantly promoted bone regeneration in the periodontal defect
scaffold implantation experiment. Moreover, LPA-loaded scaffolds were
confirmed to enhance osteogenic activities by upregulating the expression
of β-catenin and further activating the Wnt/β-catenin
pathway. These results demonstrate that the biphase PP-pDA/nHA-LPA
delivery system is a promising material for the GBR.
“… 87 In 2022, it was also found that the binding of nano-graphene oxide to lysophosphatidic acid (LPA) in serum activates LPAR6 and promotes the formation of tip vascular endothelial cells and angiogenesis through the YAP signaling pathway, which is independent of intracellular ROS alterations (demonstrated in Figure 4 ). 88 Collectively, Table 1 summarizes representative in vitro and in vivo studies of GFNs in regenerative medicine in recent years. Figure 4 NGO promoted the formation of new blood vessels in a rat model of calvarial bone defects.…”
Section: Applications Of the Gfns In Regenerative Medicinementioning
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