Electrochemically directed biofunctionalization of a lossy-mode resonance optical fiber sensor

Janik, Monika; Niedziałkowski, Paweł; Lechowicz, Katarzyna; Koba, Marcin; Sezemsky, Petr; Straňák, Vítězslav; Ossowski, Tadeusz; Śmietana, Mateusz

doi:10.1364/oe.390780

Cited by 17 publications

(6 citation statements)

References 40 publications

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“…This pair was chosen due to its high affinity and stability, ensuring strong and stable binding in different experimental conditions 7 . To assure the uniform coverage of the ITO-LMR sensor's surface and to avoid introducing additional chemical layer, e.g., silane, which could influence the final output, the biotin was electropolimerized following a procedure described in 6 . Right after the electropolymerization, the ITO-LMR electrode was immersed in a 1% BSA solution to block any nonspecific interactions on the surface.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, in most cases, such receptor layers are treated as insulating. However, in their active form (tertiary or quaternary conformation) they have an initial charge 6 . What needs to be emphasized, this charge may affect the efficiency of the receptor-target interactions (especially, when both are similarly charged), and it may influence the properties of the material on which the proteins are immobilized.…”

Section: Introductionmentioning

confidence: 99%

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Does the refractive index sensitivity matter the most? Charge of biological material and performance of label-free biosensors

Janik

Niedziałkowski

Lechowicz

et al. 2023

European Workshop on Optical Fibre Sensors (EWOFS 2023)

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The work discusses the possible impact of the electric charge of biological material on the properties of label-free biosensors, in particular those operating in dual domains, i.e., optical and electrochemical. Optical fiber lossy-mode resonance (LMR) sensors based on indium tin oxide (ITO) were investigated as label-free biosensors with a model biological receptor-target pair, i.e., biotin-avidin. Each of the used biological materials shows different properties, i.e., size, isoelectric point, and, therefore, also charge. The investigations were performed in two electrolytes with differently charged redox couples to better identify the possible influence of chargé of biological material on the optical readout. The obtained results clearly indicate that in designing label-free biosensing solutions, consideration of a broader range of biological materials properties than just refractive index, such as their charge, is required.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Does the refractive index sensitivity matter the most? Charge of biological material and performance of label-free biosensors

Janik

Niedziałkowski

Lechowicz

et al. 2023

European Workshop on Optical Fibre Sensors (EWOFS 2023)

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“…CV and EIS measurements were performed after each gold electrode's functionalization step were carried out in a 0.01 M PBS solution containing 1 mM of ([Fe-(CN) 6 ] 3À /[Fe(CN) 6 ] 4À used as redox couples. This redox mediator belongs to inner-sphere electrochemical redox probes sensitive to electrode surface changes [32,33]. Figure 2a shows the CV's obtained for the bare gold electrode, modified with cysteamine, peptide, and the response of the electrode in the presence of hCG in the concentration of 1 × 10 À 7 M (50 000 mIU/mL).…”

Section: Cyclic Voltammetrymentioning

confidence: 99%

An electrochemical biosensor for the determination of hormone Human Chorionic Gonadotropin (hCG) in human serum

et al. 2023

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This work describes the modification of a gold electrode to create an electrochemical biosensor capable of detecting human chorionic gonadotropin (hCG). The biosensor was obtained by modifying the gold electrode with cysteamine and oligopeptide (PPLRINRHILTR). The modification steps of the gold electrode were confirmed by cyclic voltammetry (CV) and impedance electrochemical spectroscopy (EIS) measurements. The conducted EIS experiments in 0.01 M PBS, pH 7.4 confirm that the biosensor exhibits sensitivity towards hCG in a range of concentrations from 1×10−12 to 1×10−7 M (0.5 mIU/mL – 50 000 mIU/mL) to solutions with a detection limit of 1. 91×10−14 M (0.0095 mIU/mL). The effectiveness of the investigated biosensor was also investigated in human serum. The EIS comparative investigations were performed in human serum containing a concentration of 1×10−12 M (0.5 mIU/mL) hCG and in human serum where the hCG was added. The obtained results indicate that the investigated biosensor is selective for the presence of hCG hormone in the human serum.

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“…It allows acquisition of extra information on the state of the WE surface and therefore, additional cross-verification of the SEC results. The ITO-LMR sensors have already been reported for monitoring of EC processes 31 , including electrodeposition 32 , 33 , as well as label-free biosensing 34 , 35 . However, as the LMR depends on properties of the nanocoating, i.e., its thickness and optical properties, but also properties of the analyte surrounding the nanocoating its application in the SEC setup may be highly beneficial 36 .…”

Section: Introductionmentioning

confidence: 99%

Enhanced spectroelectrochemistry with lossy-mode resonance optical fiber sensor

Janik,

Lechowicz,

Pituła

et al. 2023

Sci Rep

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Spectroelectrochemical (SEC) measurements play a crucial role in analytical chemistry, utilizing transparent or semitransparent electrodes for optical analysis of electrochemical (EC) processes. The EC readout provides information about the electrode's state, while changes in the transmitted optical spectrum help identify the products of EC reactions. To enhance SEC measurements, this study proposes the addition of optical monitoring of the electrode. The setup involves using a polymer-clad silica multimode fiber core coated with indium tin oxide (ITO), which serves as both the electrode and an optical fiber sensor. The ITO film is specifically tailored to exhibit the lossy-mode resonance (LMR) phenomenon, allowing for simultaneous optical monitoring alongside EC readouts. The LMR response depends on the properties of the ITO and the surrounding medium's optical properties. As a result, the setup offers three types of interrogation readouts: EC measurements, optical spectrum analysis corresponding to the volume of the analyte (similar to standard SEC), and LMR spectrum analysis reflecting the state of the sensor/electrode surface. In each interrogation path, cyclic voltammetry (CV) experiments were conducted individually with two oxidation–reduction reaction (redox) probes: potassium ferricyanide and methylene blue. Subsequently, simultaneous measurements were performed during chronoamperometry (CA) with the sensor, and the cross-correlation between the readouts was examined. Overall, this study presents a novel and enhanced SEC measurement approach that incorporates optical monitoring of the electrode. It provides a comprehensive understanding of EC processes and enables greater insights into the characteristics of the analyte.

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Electrochemically directed biofunctionalization of a lossy-mode resonance optical fiber sensor

Cited by 17 publications

References 40 publications

Does the refractive index sensitivity matter the most? Charge of biological material and performance of label-free biosensors

Does the refractive index sensitivity matter the most? Charge of biological material and performance of label-free biosensors

An electrochemical biosensor for the determination of hormone Human Chorionic Gonadotropin (hCG) in human serum

Enhanced spectroelectrochemistry with lossy-mode resonance optical fiber sensor

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