Electrochemiluminescence DNA Biosensor for HBV Based on Coralloid Poly(Aniline‐Luminol)‐MWCNTs and Catalysis of Ferrocene

Abstract

A novel electrochemiluminescence biosensor was presented for the assay of hepatitis B virus (HBV) DNA. Coralloid poly(aniline‐luminol)‐MWCNTs was prepared on an electrode surface, which was followed by the attachment of capture DNA via amide bond formation. The HBV‐DNA and ferrocene labelled DNA hybridized with capture DNA to form a sandwich structure on electrode surface. Ferrocene can effectively increase the signal output of the ECL sensor due to the catalysis of ferrocene on electrochemiluminescence of luminol. The biosensor rendered a linear dependence over the range from 1.0×10−16 to 1.0×10−7 M with a low detection limit of 2.4×10−17 M.

Exploiting poly(safranine) and poly(luminol) for sensing applications. A mini review

“…One of the latest research developments in this field, using poly(luminol–aniline)/MWCNTs as an inventive biosensor for the hepatitis B virus (HBV) DNA assay, has been published. 61 On the electrode surface, the desired coralloid poly(aniline–luminol)-has been fabricated and accompanied by the capture DNA attachment through amide bond construction. A sandwich structure was formed on the electrode surface attributable to ferrocene-labelled DNA hybridized and HBV-DNA.…”

“…10 Recently, the uses of luminol and its small molecule derivatives have been widely employed to develop sensing properties toward materials as both electrochemical and biosensors. 7,[47][48][49][50][51][52][53][54][55][56][57][58][59][60][61] Over the past few years, water-soluble dyes, such as carmine, methylene dye derivatives, and other dyes, have been broadly employed in catalyzing oxidation or reducing organic molecules as mediators. [11][12][13] Safranine [Fig.…”

“…10 Recently, the uses of luminol and its small molecule derivatives have been widely employed to develop sensing properties toward materials as both electrochemical and biosensors. 7,47–61…”

Sensitive Cr3+ sensor based on novel poly(luminol-co-1,8-diaminonaphthalene)/CeO2/MWCNTs nanocomposites

“…This positively impacts the electrochemical properties of poly(luminol) and its potential use in electrochemical and biosensor materials. [5][6][7][8][9][10] From the same family, a new type of multifunctional polymer material, poly(di-aminonaphthalene), can be synthesized from aromatic diamines, such as 1,5-, 1,8-, or 2,3-diaminonaphthalene, through electrochemical or chemical oxidative polymerization with properties similar to those of polyaniline and polypyrrole. 11 The substance 1,8-diaminonaphthalene displays properties such as electroconductivity, electrocatalysis, electroactivity, permselectivity, electrochromism, and other properties that stem from the chemical reactivity of the functional amino groups on its macromolecular structure.…”

“…This positively impacts the electrochemical properties of poly(luminol) and its potential use in electrochemical and biosensor materials. 5–10…”