Electrochemiluminescence Single‐Cell Analysis: Intensity‐ and Imaging‐Based Methods

Ding, Heping; Guo, Weiliang; Su, Bin

doi:10.1002/cplu.202000145

Cited by 36 publications

(24 citation statements)

References 107 publications

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“…[17] Novel ECL strategies, including capacitance ECL microscopy, [18] co-reactantembedded [19] and bio-co-reactant-enhanced imaging, [20] ECL photobleaching [21] have also been developed, which promise even more possibilities in imaging molecules, structures and biological events by ECL. [22] But practical imaging applications usually need to probe different structures, for example cell-matrix and cell-cell junctions, in one sample like CLSM, which remains challenging for most of ECL imaging methods reported hitherto. [23] In this work, we report spatially selective ECL imaging of cell-matrix and cell-cell junctions by rationally modulating the thickness of ECL layer.…”

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confidence: 99%

Spatially Selective Imaging of Cell–Matrix and Cell–Cell Junctions by Electrochemiluminescence

Ding

Zhou

et al. 2021

Angewandte Chemie

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Cell junctions are protein structures located at specific cell membrane domains that determine key processes in multicellular development. Here we report spatially selective imaging of cell junctions by electrochemiluminescence (ECL) microscopy. By regulating the concentrations of luminophore and/or co‐reactant, the thickness of ECL layer can be controlled to match with the spatial location of different cell junctions. At a low concentration of luminophore, ECL generation is confined to the electrode surface, thus revealing only cell–matrix adhesions at the bottom of cells. While at a high concentration of luminophore, the ECL layer can be remarkably extended by decreasing the co‐reactant concentration, thus allowing the sequential imaging of cell–matrix and cell–cell junctions at the bottom and near the apical surface of cells, respectively. This strategy not only provides new insights into the ECL mechanisms but also promises wide applications of ECL microscopy in bioimaging.

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confidence: 99%

Spatially Selective Imaging of Cell–Matrix and Cell–Cell Junctions by Electrochemiluminescence

Ding

Zhou

et al. 2021

Angewandte Chemie

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“…ECL imaging strategies are operated in near-dark conditions and circumvent most issues such as autofluorescence or saturation of the fluorophore absorption. It has popularized ECL as a quantitative optical readout for bioanalysis [46] and more recently as a promising optical imaging tool [47,48]. To apprehend the opportunities afforded by ECL microscopy, different reviews detail the mechanisms implied in ECL reactions [49].…”

Section: Photon Budgetmentioning

confidence: 99%

Electrochemistry and Optical Microscopy

Kanoufi

2021

Encyclopedia of Electrochemistry

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Electrochemistry exploits local current heterogeneities at various scales ranging from the micrometer to the nanometer. The last decade has witnessed unprecedented progress in the development of a wide range of electroanalytical techniques allowing to reveal and quantify such heterogeneity through multiscale and multifonctionnal operando probing of electrochemical processes. However most of these advanced electrochemical imaging techniques, employing scanning probes, suffer from either low imaging throughput or limited imaging size. In parallel, optical microscopies, which can image a wide field of view in a single snapshot, have made considerable progress in terms of sensitivity, resolution and implementation of detection modes. Optical microscopies are then mature enough to propose, with basic bench equipment, to probe in a non destructive way a wide range of optical (and therefore structural) properties of a material in situ, in real time: under operating conditions. They offer promising alternative strategies for quantitative high-resolution imaging of electrochemistry. The first sections recall the optical properties of materials and how they can be probed optically. They discuss fluorescence, Raman, surface plasmon resonance, scattering or refractive index. Then the different optical microscopes used to image electrochemical processes are examined along with some strategies to extract quantitative electrochemical information from optical images. Finally the last section reviews some examples of in situ imaging, at micro-to nanometer resolution, and quantification of electrochemical processes ranging from solution diffusion to the conversion of molecular interfaces or solids.]

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“…Recently, excellent reviews regarding ECL have been published, including a minireview on the ECL single-cell analysis that has emerged in recent years [9] and a review on pathogenic bacteria detection [10]. In contrast, in this present review, recent studies ranging from single-cell systems to cell aggregates and cell secretions, focusing on mammalian cells, are reviewed to give a comprehensive summary.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Electrochemiluminescence-Based Systems for Mammalian Cell Analysis

et al. 2020

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Mammalian cell analysis is essential in the context of both fundamental studies and clinical applications. Among the various techniques available for cell analysis, electrochemiluminescence (ECL) has attracted significant attention due to its integration of both electrochemical and spectroscopic methods. In this review, we summarize recent advances in the ECL-based systems developed for mammalian cell analysis. The review begins with a summary of the developments in luminophores that opened the door to ECL applications for biological samples. Secondly, ECL-based imaging systems are introduced as an emerging technique to visualize single-cell morphologies and intracellular molecules. In the subsequent section, the ECL sensors developed in the past decade are summarized, the use of which made the highly sensitive detection of cell-derived molecules possible. Although ECL immunoassays are well developed in terms of commercial use, the sensing of biomolecules at a single-cell level remains a challenge. Emphasis is therefore placed on ECL sensors that directly detect cellular molecules from small portions of cells or even single cells. Finally, the development of bipolar electrode devices for ECL cell assays is introduced. To conclude, the direction of research in this field and its application prospects are described.

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Electrochemiluminescence Single‐Cell Analysis: Intensity‐ and Imaging‐Based Methods

Cited by 36 publications

References 107 publications

Spatially Selective Imaging of Cell–Matrix and Cell–Cell Junctions by Electrochemiluminescence

Spatially Selective Imaging of Cell–Matrix and Cell–Cell Junctions by Electrochemiluminescence

Electrochemistry and Optical Microscopy

Recent Advances in Electrochemiluminescence-Based Systems for Mammalian Cell Analysis

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