Electrochemiluminoimmunoassay of hTSH at disposable oxide-coated n-silicon electrodes

Helin, Mika; Väre, L.; Håkansson, Markus; Canty, Philip; Hedman, Hannu‐Pekka; Heikkilä, L.; Ala-Kleme, Timo; Kankare, Jouko; Kulmala, Sakari

doi:10.1016/s0022-0728(02)00638-1

Cited by 28 publications

(10 citation statements)

References 23 publications

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“…We assume that oxide-coated silicon electrodes [56] can also be applied as working electrodes for the cathodic excitation of fluorescein, which will be studied in the very near future in our laboratory. Oxide-coated silicon working electrodes are very fascinating if one is aiming at novel microanalytical devices.…”

Section: Discussionmentioning

confidence: 99%

Hot electron-induced electrochemiluminescence of fluorescein in aqueous solution

Suomi¹,

Ylinen²,

Håkansson³

et al. 2006

Journal of Electroanalytical Chemistry

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Section: Discussionmentioning

confidence: 99%

Hot electron-induced electrochemiluminescence of fluorescein in aqueous solution

Suomi¹,

Ylinen²,

Håkansson³

et al. 2006

Journal of Electroanalytical Chemistry

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“…Helin et al 82 introduced an ECL immunoassay for a human thyroidstimulating hormone (hTSH) on oxide-coated n-silicon electrodes by utilizing the Tb(III) chelate as a label. The calibration curve of the immunometric hTSH assay was found to be linear over a wide range of hTSH concentration, and the detection limit of the hTSH was below 4 pM.…”

Section: ·6·2 Other Ecl Labelsmentioning

confidence: 99%

Chemiluminescence Platforms in Immunoassay and DNA Analyses

Fan

Cao

et al. 2009

ANAL. SCI.

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IntroductionCL is the production of electromagnetic radiation by a chemical reaction. The CL intensity is directly proportional to the concentration of a limiting reactant involved in the CL reaction. CL has been exploited for its use in a wide range of applications due to its high sensitivity, wide calibration range, and suitability for miniaturization. Protein and DNA analyses have recently attracted much attention. Many different types of techniques, such as electrochemical, optical, radiochemical, and piezoelectronic methods have been applied for protein and DNA analyses. To provide an overview of CL detection assays for DNAs and proteins, we have summarized their essential techniques. Monoplex and multiplex assays are described, and their current reports are intended to provide an overview of the CL techniques currently employed in numerous laboratories. Monoplex AssayA monoplex assay aims to measure the presence of a single analyte in any given sample, in which nanoparticle, DNA enzyme, horseradish peroxidase (HRP), alkaline phosphatase (AP) and acridinium ester are commonly used as labels. 2·1 Nanoparticle-based CL techniquesNanoparticles offer excellent prospects for DNA detection and immunoassay, owing to their many attractive properties. Compared to existing labels, nanoparticles are more stable and cheaper, and easier to be purified. In addition, they indicate faster binding kinetics with high sensitivity and a high reaction rate for many types of monoplex assays, ranging from immunoassays to DNA detection. 2·1·1 Gold nanoparticlesColloidal gold labels are ideal in biotechnological systems for several reasons. Firstly, they can be readily prepared in a wide range of sizes, from approximately 2 nm to above 100 nm. Secondly, the biochemical activity of the labeled biomolecules can be retained when colloidal gold is coupled to the biomolecules and, thirdly, colloidal gold labels can be easily visualized as dense structures within biological entities using transmission electron microscopy. As a biological tag, colloidal gold has been employed in several CL detection systems. 2·1·1·1 Gold nanoparticle-based CL immunoassay. The Au 3+ -luminol reaction, in which 10 -9 M Au 3+ ions can be sensitively detected, is a classic CL reaction. Thousands of Au atoms are contained within the gold nanoparticles; for example, 2.3 ¥ 10 5 Au atoms are theoretically contained in a 20-nm spherical gold particle and, consequently, picomolar detection limits can be attained by employing oxidative gold metal dissolution in acidic solutions. Our group 1,2 reported a sensitive CL immunoassay based on a colloidal gold label after oxidative gold metal dissolution (using 0.01 M HCl-0.5 M NaCl-0.5 mM Br2), using a simple and sensitive luminol-CL reaction (Fig. 1). Reviews Chemiluminescence Platforms in Immunoassay and DNA AnalysesAiping FAN,* Zhijuan CAO,* Huan LI,* Masaaki KAI,** and Jianzhong LU* † *School of Pharmacy, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China ** Faculty of Pharmaceutical Sciences, Graduate Scho...

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“…Various luminophores (e.g., Tb(III) chelates), organic fluorophores and Ru(bpy) 3 2+ have been studied when HECL excitation mechanisms and new electrode materials have been developed [11,12,13,14]. Tb(III) chelates have been the main label in bioaffinity assays of C-reactive protein [4,11,13,15], β 2 ‑Microglobulin [16] and human thyroid stimulating hormone [12,17,18]. Some aromatic Tb(III) chelates exhibit high emission intensity and a relatively long luminescence lifetime which enables time-resolved detection and the use of materials generating relatively high background emission during cathodic excitation pulses in real-world assays, such as, oxide-covered aluminum electrodes.…”

Section: Introductionmentioning

confidence: 99%

Immunoassays Based on Hot Electron-Induced Electrochemiluminescence at Disposable Cell Chips with Printed Electrodes

Grönroos

Nur-E-Habiba

Salminen

et al. 2019

Sensors

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Novel hot electron-emitting working electrodes and conventional counter electrodes were created by screen printing. Thus, low-cost disposable electrode chips for bioaffinity assays were produced to replace our older expensive electrode chips manufactured by manufacturing techniques of electronics from silicon or on glass chips. The present chips were created by printing as follows: (i) silver lines provided the electronic contacts, counter electrode and the bottom of the working electrode and counter electrode, (ii) the composite layer was printed on appropriate parts of the silver layer, and (iii) finally a hydrophobic ring was added to produce the electrochemical cell boundaries. The applicability of these electrode chips in bioaffinity assays was demonstrated by an immunoassay of human C-reactive protein (i) using Tb(III) chelate label displaying long-lived hot electron-induced electrochemiluminescence (HECL) and (ii) now for the first time fluorescein isothiocyanate (FITC) was utilized as an a low-cost organic label displaying a short-lived HECL in a real-world bioaffinity assay.

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Electrochemiluminoimmunoassay of hTSH at disposable oxide-coated n-silicon electrodes

Cited by 28 publications

References 23 publications

Hot electron-induced electrochemiluminescence of fluorescein in aqueous solution

Hot electron-induced electrochemiluminescence of fluorescein in aqueous solution

Chemiluminescence Platforms in Immunoassay and DNA Analyses

Immunoassays Based on Hot Electron-Induced Electrochemiluminescence at Disposable Cell Chips with Printed Electrodes

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