Electrochemistry of the polypyrrole glucose oxidase electrode

Bélanger, Daniel; Nadreau, Jocelyn; Fortier, Guy

doi:10.1016/0022-0728(89)87036-6

Cited by 240 publications

(61 citation statements)

References 30 publications

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“…The characteristic sigmoidal current± potential curves are obtained with limiting currents of 25X4 mA and 11X8 mA for DMAFc and ferrocene, respectively. The current±potential relationship of the Na®on-GOx-DMAFc system tracks closely that of electrodes based on the electrochemical oxidation of hydrogen peroxide [54,55]. On the other hand, the Na®on-GOx-Fc electrode is characterized by a current± potential curve that is shifted to more negative potential in comparison with that of the Na®on-GOx-DMAFc electrode.…”

Section: Amperometric Current±potential Relationshipmentioning

confidence: 83%

Electrochemical and Enzymatic Studies of Electron Transfer Mediation by Ferrocene Derivatives with Nafion-Glucose Oxidase Electrodes

et al. 1999

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Na®on-glucose oxidase-ferrocene electrodes have been used to study the electron transfer between an electrode and the active site of the enzyme. These electrodes were prepared by casting a mixture of Na®on and glucose oxidase, GOx, (both dissolved in methanol) at the surface of a platinum disk electrode. The ferrocene mediators were incorporated by either soaking PtyNa®on and PtyNa®on-GOx electrodes in an aqueous solutions of N,N H -dimethylaminomethylferrocene, DMAFc, or by depositing an aliquot of a ferrocene solution at the surface of the enzyme ®lm electrode. The Na®on-GOx electrodes are characterized by a slower incorporation rate of the ferrocene than the plain Na®on electrode indicating that the af®nity of Na®on for the ferrocene derivative is increased by the addition of the enzyme. Accordingly, the ion-exchange distribution coef®cient is larger for plain Na®on (3.2610 3 ) than for Na®on-GOx (7.2610 2 ). The cyclic voltammetry of the PtyNa®on-GOx electrode in the presence of glucose and in deaerated solution show a well developed catalytic wave indicating that the ferrocene acts as a mediator for the electron transfer between the enzyme and the platinum electrode. For a high glucose concentration (75 mM), the amperometric response of these electrodes increased and the apparent diffusion coef®cient DMAFc decreased with an increase of DMAFc concentration in the enzymatic layer. These observations suggest that the glucose response is limited by the probability of an encounter between the reduced enzyme and the oxidized ferrocene rather than by the charge transfer between the reduced ferrocene and the electrode. The effective Michaelis constant, K m , for glucose and DMAFc were evaluated and values of 25 mM and 4 nmolycm 2 were obtained, respectively.

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Section: Amperometric Current±potential Relationshipmentioning

confidence: 83%

Electrochemical and Enzymatic Studies of Electron Transfer Mediation by Ferrocene Derivatives with Nafion-Glucose Oxidase Electrodes

et al. 1999

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“…Before use, cyclic voltammetry wa performed in 0.1M aqueous KC1 to electrochemically characterize the polypyrrole films. 4 The coated electrode was stored in phosphate buffer solution at 4°C when not used. The total charge (mC/cm2) is evaluated from the area under the anodic currenttime trace recorded during the formation of the film.…”

Section: Ppigod Film Preparationmentioning

confidence: 99%

Characterization of the biochemical behavior of glucose oxidase entrapped in a polypyrrole film

Fortier

Bélanger

1991

Biotech & Bioengineering

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This article reports the characterization of the biochemical behavior of glucose oxidase entrapped in polypyrrole. The immobilization of glucose oxidase in a polypyrrole film was performed by entrapment during the electropolymerization of pyrrole at a platinum electrode poised at 0.65 V vs. SCE in aqueous solution in a one-compartment electrochemical cell. Thin films of polypyrrole (0.11 microm) were obtained and the entrapped enzyme obeyed Michaelis kinetics, indicating no diffusional constraints of the substrate. Our results indicate that the entrapped glucose oxidase is more resistant to denaturation conditions such as alkaline pH and temperature (50 and 60 degrees C) than the soluble form of the enzyme. The autoinactivation constant for the entrapped enzyme was also determined in presence of 0.25M of glucose and was 6.19 x 10(-4) min(-1), i.e., corresponding to a half-life value of 20 h. The results reported here show clearly that polypyrrole matrix has a strong stabilizing effect on the stucture and on the activity of glucose oxidase.

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“…Moreover, it was observed that PANI is able to interplay with H 2 . There was reported immediate interaction of H 2 with doped polyaniline nanofibers to provoke a modification in the conductivity of the nanofibers [34]. The direct mass uptake of H 2 by polyaniline nanofibers was found as well by a quartz crystal microbalance (ca.…”

Section: Chemical Sensorsmentioning

confidence: 87%

“…3% relative to the nanofiber mass). A reliable program of the H 2 /PANI reaction provokes H 2 interacting with doped PANI at the charged amine nitrogen, proceeded after the dissociation of H 2 and the creation of N-H bonds at the amine nitrogen of the polyaniline [34,35]. Afterward, charge transport between adherent amine nitrogen atoms regains the polyaniline back to its classical doped, emeraldine-salt pattern with a deliverance of H 2 .…”

Section: Chemical Sensorsmentioning

confidence: 99%

Conducting Polymers in Sensor Design

Sołoducho¹,

Cabaj²

2016

Conducting Polymers

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Conducting polymers (CPs) as well as conducting polymer nanoparticles seem to be very applicable for the development of various analyte-recognizing elements of sensors and biosensors. This chapter reviews mainly fabrication methods as well as application of conducting polymers in sensors. Conducting polymers (CPs) have been applied in the design of catalytic and affinity biosensors as immobilization matrixes, signal transduction systems, and even analyte-recognizing components. Various types of conducting and electrochemically generated polymer-based electrochemical sensors were developed including amperometric catalytic and potentiodynamic affinity sensors. A very specific interaction of analyte with immobilized biological element results in the formation of reaction products.

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Electrochemistry of the polypyrrole glucose oxidase electrode

Cited by 240 publications

References 30 publications

Electrochemical and Enzymatic Studies of Electron Transfer Mediation by Ferrocene Derivatives with Nafion-Glucose Oxidase Electrodes

Electrochemical and Enzymatic Studies of Electron Transfer Mediation by Ferrocene Derivatives with Nafion-Glucose Oxidase Electrodes

Characterization of the biochemical behavior of glucose oxidase entrapped in a polypyrrole film

Conducting Polymers in Sensor Design

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