Electrodeposited graphene nano-stacks for biosensor applications. Surface groups as redox mediators for laccase

Świetlikowska, Agnieszka; Gniadek, Marianna; Pałys, Barbara

doi:10.1016/j.electacta.2013.03.055

Cited by 25 publications

(29 citation statements)

References 40 publications

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“…Such the biocathode exhibited only a negligible j = 2.6 μA cm −2 (Fig. 1A), which is in a perfect agreement with j ≈ 2.5 μA cm −2 reported on the ErGO-decorated biocathode with physically adsorbed laccase [47]. Even though laccase can be effectively docked on hydrophobic surfaces [46,53], the presence of carboxyl groups can be beneficial for binding of BOD in a way allowing fast electron transfer [35].…”

Section: Resultssupporting

confidence: 86%

“…The main prerequisite for construction of effective and miniaturised BFCs is to establish an effective electron transfer route between the biocatalyst and the electrode [44,45]. Different graphene derivatives are known to promote such electron exchange with enzymes including covalently attached [46] and even physisorbed [47] laccase. In our previous work we showed a bioelectrocatalysis of BOD incubated with GO for the first time [48].…”

Section: Introductionmentioning

confidence: 99%

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Effective bioelectrocatalysis of bilirubin oxidase on electrochemically reduced graphene oxide

Filip

2014

Electrochemistry Communications

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Section: Resultssupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Effective bioelectrocatalysis of bilirubin oxidase on electrochemically reduced graphene oxide

Filip

2014

Electrochemistry Communications

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“…GO was synthesized from graphite powder (Sigma Aldrich; 1-2 µm, synthetic) through the modified Hummers-Offeman method 8,39 .…”

Section: Methodsmentioning

confidence: 99%

Electrochemically Reduced Graphene Oxide on Electrochemically Roughened Gold as a Support for Horseradish Peroxidase

et al. 2014

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Graphene oxide (GO) is composed of isolated graphene-like sheets containing various oxygen functionalities. The electrochemical reduction of GO enables the direct deposition of the graphene layer on electrode surfaces. Furthermore, a partial reduction of GO can be performed, enabling easy modification of the surface with oxygen-containing groups. In this contribution, the electrochemical reduction of GO is carried out on a gold electrode roughened by electrochemical oxidation-reduction cycles. Such a gold electrode contains nanostructures ranging in size from tens to 300 nm. The electrochemically reduced GO layer is subsequently used for horseradish peroxidase (HRP) immobilization. Two types of electrochemically reduced GOs, partially reduced graphene oxide (ERGOP) and fully reduced graphene oxide (ERGOF), are used in this study. Although HRP immobilized on both types of surfaces directly catalyseselectroreduction of the hydrogen peroxide, the enzyme immobilized on the ERGOP layer exhibits slightly higher current values compared with those of ERGOF. In contrast, HRP adsorbed directly on the roughened gold reveals negligible activity. To evaluate the effect of the roughened gold, HRP immobilized on an ERGOF layer on glassy carbon was studied.Additionally, infrared and Raman spectroscopy were used to investigate the composition of GO, ERGOP, and ERGOF as well as the HRP structure at the studied surfaces. The infrared results indicate a random orientation of the enzyme molecules on the bare roughened gold and ordered HRP layers on the surfaces covered with either ERGOP or ERGOF.

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“…The electrochemical reduction process of GO on the electrode surface was described earlier as a fast, simple and universal method of obtaining graphene sheets [4,[28][29]. Moreover, the safe and harmless synthesis in comparison to the chemical reduction procedure (utilizing poisonous reagents) is undoubtedly an advantage [30].…”

Section: Electrochemical Reduction Of Go/mwcnt/pyba and Physicochemicmentioning

confidence: 99%

Spatial Architecture of Modified Carbon Nanotubes/Electrochemically Reduced Graphene Oxide Nanomaterial for Fast Electron Transfer. Application in Glucose Biosensor

Jakubow‐Piotrowska

Kowalewska

2019

Electroanalysis

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Electron transfer (ET) reactions in bioelectrocatalysis of enzymes at electrode surfaces require not only the efficient immobilization, but also highly conductive nanostructured platform, which allows for retaining its bioactivity and structural conformation. The novel architecture of spatially separated electrochemically reduced graphene oxide (ERGO) by multi‐walled carbon nanotubes functionalized with 4‐(pyrrole‐1‐yl) benzoic acid (MWCNT/PyBA) with the accurate porous structure could be an alternative for earlier approaches to the construction of bioelectrocatalytic systems with rapid diffusion of reagents from the solution to the enzyme molecule. The formation of ERGO/MWCNT/PyBA system was confirmed by electrochemical, spectroscopic and microscopic methods. The cyclic voltammetry experiments revealed that the presence of ERGO in the conductive material affects the electronic communication between the enzyme molecule and modified electrode surface greatly improving its ET properties resulting in a double increase of the heterogeneous ET rate constant value (ks=6.5 s−1). The fabricated glucose oxidase based biosensor sensitively detects glucose, therefore, ERGO/MWCNT/PyBA architecture could provide a novel and efficient platform for immobilization of redox enzymes.

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Electrodeposited graphene nano-stacks for biosensor applications. Surface groups as redox mediators for laccase

Cited by 25 publications

References 40 publications

Effective bioelectrocatalysis of bilirubin oxidase on electrochemically reduced graphene oxide

Effective bioelectrocatalysis of bilirubin oxidase on electrochemically reduced graphene oxide

Electrochemically Reduced Graphene Oxide on Electrochemically Roughened Gold as a Support for Horseradish Peroxidase

Spatial Architecture of Modified Carbon Nanotubes/Electrochemically Reduced Graphene Oxide Nanomaterial for Fast Electron Transfer. Application in Glucose Biosensor

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