Electrodeposition of alginate gels for construction of vascular-like structures

Ozawa, Fumisato; Ino, Kosuke; Takahashi, Yasufumi; Shiku, Hitoshi; Matsue, Tomokazu

doi:10.1016/j.jbiosc.2012.10.014

Cited by 63 publications

(61 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Fluidic channels can also be formed in hydrogels by depositing the cell-laden alginate solution around a metal rod [31] or a Pt wire electrode [32] followed by removal of the templates to create hollow lumen in the vascular structure, but this method is able only to generate a single straight lumen with uniform size and morphology.…”

Section: Introductionmentioning

confidence: 99%

Coaxial nozzle-assisted 3D bioprinting with built-in microchannels for nutrients delivery

et al. 2015

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Coaxial nozzle-assisted 3D bioprinting with built-in microchannels for nutrients delivery

et al. 2015

View full text Add to dashboard Cite

“…Xu and coworkers [23] designed and platform-assisted inkjet printing system to fabricate 3D zigzag cellular tubes vertically by fusion of sodium alginate droplet encapsulating 3T3 fibroblast cells. Ozawa et al [24] applied an electrodeposition technique to deposit cellladen alginate solution around Pt wire electrode and pulled out the electrode later to create hollow lumen of the vascular structure. Multiple cell types were deposited around the wire using that method.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Cell Viability and Functionality in Vessel-like Bioprintable Cell-Laden Tubular Channels

Zhang

Martin

et al. 2013

Journal of Biomechanical Engineering

238

147

View full text Add to dashboard Cite

Organ printing is a novel concept recently introduced in developing artificial threedimensional organs to bridge the gap between transplantation needs and organ shortage. One of the major challenges is inclusion of blood-vessellike channels between layers to support cell viability, postprinting functionality in terms of nutrient transport, and waste removal. In this research, we developed a novel and effective method to print tubular channels encapsulating cells in alginate to mimic the natural vascular system. An experimental investigation into the influence on cartilage progenitor cell (CPCs) survival, and the function of printing parameters during and after the printing process were presented. CPC functionality was evaluated by checking tissue-specific genetic marker expression and extracellular matrix production. Our results demonstrated the capability of direct fabrication of cell-laden tubular channels by our newly designed coaxial nozzle assembly and revealed that the bioprinting process could induce quantifiable cell death due to changes in dispensing pressure, coaxial nozzle geometry, and biomaterial concentration. Cells were able to recover during incubation, as well as to undergo differentiation with high-level cartilage-associated gene expression. These findings may not only help optimize our system but also can be applied to biomanufacturing of 3D functional cellular tissue engineering constructs for various organ systems.

show abstract

“…91 The method involves electrodeposition of hydrogel around a wire electrode. The general architecture is shown in Fig.…”

Section: Dep-based Devices For Bioanalysismentioning

confidence: 99%

Microchemistry- and MEMS-based Integrated Electrochemical Devices for Bioassay Applications

Ino

2015

Electrochemistry

Self Cite

View full text Add to dashboard Cite

Here, we present an overview of our recent research progress in development of electrochemical devices/systems for bioassays. These devices/systems are based on microchemistry and micro-electromechanical systems (MEMS) for sophisticated analytical and electrochemical measurements. In particular, we exploit the unique chemical reactions occurring in micrometer-size spaces and/or involving micrometer-size structures for bioassays, including cell analyses. This paper addresses five topics: probe-, chip-, large-scale-integration chip-, and dielectrophoresisbased devices, and electrodeposition of hydrogels for bioassays.

show abstract

Electrodeposition of alginate gels for construction of vascular-like structures

Cited by 63 publications

References 12 publications

Coaxial nozzle-assisted 3D bioprinting with built-in microchannels for nutrients delivery

Coaxial nozzle-assisted 3D bioprinting with built-in microchannels for nutrients delivery

Evaluation of Cell Viability and Functionality in Vessel-like Bioprintable Cell-Laden Tubular Channels

Microchemistry- and MEMS-based Integrated Electrochemical Devices for Bioassay Applications

Contact Info

Product

Resources

About