Electrofilament deposition and off-column detection of analytes separated by capillary electrophoresis

DeVault, Gerald L.

doi:10.1002/(sici)1522-2683(20000401)21:7<1320::aid-elps1320>3.0.co;2-j

Cited by 12 publications

(12 citation statements)

References 26 publications

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“…Electro-filament deposition system. The CE deposition system was described in full in a previous w x publication 22 . Briefly, the EF deposition system uses an interface with a coaxial liquid sheath arrangement to deposit the effluent from a capillary Ž .…”

Section: Methodsmentioning

confidence: 99%

Two‐dimensional capillary electrophoresis‐thin layer chromatography separations of amino acid enantiomers using electro‐filament transfer

DeVault

2000

J. Micro. Sep.

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Section: Methodsmentioning

confidence: 99%

Two‐dimensional capillary electrophoresis‐thin layer chromatography separations of amino acid enantiomers using electro‐filament transfer

DeVault

2000

J. Micro. Sep.

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“…Capillary electrospray has been reported for MALDI target preparation following CE [54,55], RP LC [56], and sizeexclusion chromatography [57]. As a sample preparation technique, electrospray yields uniform sample films [58][59][60] and can markedly improve the homogeneity of sample on the target surface, thereby enabling improved quantitative analysis [37,61].…”

Section: Off-chip Maldi Target Preparationmentioning

confidence: 99%

Microfluidic technologies for MALDI‐MS in proteomics

DeVoe

Lee

2006

Electrophoresis

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The field of microfluidics continues to offer great promise as an enabling technology for advanced analytical tools. For biomolecular analysis, there is often a critical need to couple on-chip microfluidic sample manipulation with back-end MS. Though interfacing microfluidics to MS has been most often reported through the use of direct ESI-MS, there are compelling reasons for coupling microfluidics to MALDI-MS as an alternative to ESI-MS for both online and offline analysis. The intent of this review is to provide a summary of recent developments in the integration of microfluidic systems with MALDI-MS, with an emphasis on applications in proteomics. Key points are summarized, followed by a review of relevant technologies and a discussion of outlook for the field.

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“…The CE system was described in full elsewhere [29]. Briefly, the CE apparatus was constructed in-house using a 30-kV high-voltage power supply (Gamma High Voltage Research, Ormond Beach, FL, USA) operated in constant voltage mode.…”

Section: Ce Separationsmentioning

confidence: 99%

“…In the work reported herein, a modification of electrospray spatially focuses the effluent from a CE capillary onto planar substrates [29,30]. Conditions are optimized to produce an electrofilament (EF), which is a narrow liquid filament (ca.…”

Section: Introductionmentioning

confidence: 99%

Spatially focused deposition of capillary electrophoresis effluent onto surface-enhanced Raman-active substrates for off-column spectroscopy

DeVault

2001

Electrophoresis

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Surface-enhanced Raman scattering (SERS) is employed to obtain distinctive spectra of compounds that are efficiently separated by capillary electrophoresis (CE) and deposited onto planar SERS-active substrates. A simple method is described that explains how to prepare SERS-active substrates by depositing a silver-colloid solution onto frosted-glass microscope slides, using a high-efficiency nebulizer. Scanning electron micrographs reveal a layered coating of fairly uniform-sized, 100-nm silver nanoparticles with interstitial spaces ranging from a few to tens of nanometers. The on-column separation is monitored by laser-induced fluorescence, while electrofilament depositing the CE effluent onto a moving SERS-substrate. Subsequently, the SERS spectra and off-column electropherograms are obtained with a simple confocal Raman spectrometer. The test compounds used to demonstrate this technique include compounds of biological significance: benzyloxyresorufin, riboflavin, and resorufin. CE and Raman conditions are evaluated to determine their affects on the SERS signals. An average off-column efficiency of 100,000 plates/m and a signal reproducibility of 11% relative standard deviation were achieved. Characteristic spectra with major Raman bands exhibiting signal-to-noise ratios of greater than 3 were obtained for a 3.2-nL injection of 10(-6) M (706 fg) resorufin. Forming a self-assembled monolayer (SAM) on the substrate increases the sensitivity of the SERS technique and decreases the on-substrate broadening. Calibration plots for both plain- and SAM-SERS substrates are demonstrated.

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Electrofilament deposition and off-column detection of analytes separated by capillary electrophoresis

Cited by 12 publications

References 26 publications

Two‐dimensional capillary electrophoresis‐thin layer chromatography separations of amino acid enantiomers using electro‐filament transfer

Two‐dimensional capillary electrophoresis‐thin layer chromatography separations of amino acid enantiomers using electro‐filament transfer

Microfluidic technologies for MALDI‐MS in proteomics

Spatially focused deposition of capillary electrophoresis effluent onto surface-enhanced Raman-active substrates for off-column spectroscopy

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