Electrofusion for the production of somatic hybrid plants of Solanum melongena L. and Solanum khasianum C.B. Clark

Sihachakr, D.; Haïcour, R.; Serraf, I.; Barrientos, Edilberto; Herbreteau, Catherine; Ducreux, G.; Rossignol, L.; Souvannavong, Vongsay

doi:10.1016/0168-9452(88)90127-6

Cited by 67 publications

(17 citation statements)

References 23 publications

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“…For protoplast fusions leaves were taken from four to five week-old in vitro plants. Protoplasts were prepared according to Sihachakr et al (1988) and applying 1% (w/v) cellulase R-10 (Yakult Co., Japan) and 0.2% (w/v) macerozyme R-10 (Yakult Co., Japan) for digestion of the cellular walls. The purified protoplasts were suspended in 0.5 M mannitol solution at a density of 3.5 x 105/ml.…”

Section: Methodsmentioning

confidence: 99%

Transfer of PLRV resistance fromSolanum verrucosum Schlechdt to potato (S. tuberosum L.) by protoplast electrofusion

et al. 2000

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SummarySomatic fusions between an accession of the diploid wild species Solanum verrucosum and a dihaploid S. tuberosum genotype were produced in order to incorporate resistance to potato leafroll virus (PLRV). In total 15 somatic hybrids out of 16 regenerants were Qbtained. Identification of hybrids was based on additive RAPD patterns, general morphological characteristics, chromosome numbers and chloroplast counts in stomata guard cells. A field trial was performed with the hybrids, their two parents and the control cultivar Kennebec to assess field performance and phenotypic variability. Yield parameters varied considerably among somatic hybrids. Some of the hybrids gave significantly higher yields, tuber numbers and tuber weights than both parents. Pollen fertility of hybrids ranged from 19 to 77%. Twelve hybrids were found to be resistant to PLRV.

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Section: Methodsmentioning

confidence: 99%

Transfer of PLRV resistance fromSolanum verrucosum Schlechdt to potato (S. tuberosum L.) by protoplast electrofusion

et al. 2000

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“…In the case of eggplant (Solanum melongena), sexual crossing barriers have limited the possibility of transferring agronomically-important traits such as resistance to insect attack, or resistance to various diseases to eggplant from related wild species (Daunay et al ., 1991) .. Symmetric somatic hybrids between eggplant and S. aethiopicum (Daunay et al ., 1993), S. integri-223 folium (Kameya et al, 1990), S. khasianum (Sihachakr et al ., 1988), S. nigrum (Guri & Sink, 1988a), S . sisymbrifolium (Gleddie et al ., 1986) and S .…”

Section: Eggplantmentioning

confidence: 99%

The potential of somatic hybridization in crop breeding

Waara

Glimelius

1995

Euphytica

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In recent years, the rapid development of somatic cell genetics has made possible the transfer of alien genes over wide taxonomic distances by somatic hybridization . In this review, the potential of somatic hybridization in the breeding of crops within the Brassicaceae, Fabaceae, Poaceae and Solanaceae is discussed . It is evident from these studies that many hybrids, either symmetric or asymmetric, which are fertile have the potential to be used as a bridge between the alien species and the crop . Progeny analysis of some hybrid combinations also reveals intergenomic translocations which may lead to the introgression of the alien genes . Furthermore, fusion techniques enable the resynthesis of allopolyploid crops to increase their genetic variability and to restore ploidy level and heterozygosity after breeding at reduced ploidy level in polyploid crops.

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“…The electrical apparatus and fusion procedure described by Sihachakr et al (1988) were used. The movable multi-electrodes were placed in a 15 × 50 mm Petri dish containing 500-700 ~tl aliquot of a mixture ( 1:1) of protoplasts from both parents.…”

Section: Electrofusion Apparatus and Fusion Proceduresmentioning

confidence: 99%

“…Cytological analysis was done on root tips (5 roots/hybrid clone) taken from in vitro plants as described by Sihachakr et al (1988).…”

Section: Cytological Analysismentioning

confidence: 99%

Production and characterization of intergeneric somatic hybrids through protoplast electrofusion between potato (Solanum tuberosum) and Lycopersicon pennellii

Sherraf

Tizroutine

Chaput

et al. 1994

Plant Cell Tiss Organ Cult

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Mesophyll protoplasts of Lycopersicon pennelli Corr., a wild relative of tomato, were electrofused with those from a dihaploid potato clone, cv Nicola, with the objectives of transferring saline tolerance from L. penneUii to cultivated potato. 150 calli were selected from the fusion experiments, finally giving 2 hybrid shoots. Their hybrid nature was verified by examining isoenzyme patterns for esterases (EST), peroxidase (PRX), phosphogluconate dehydrogenase (6-PGD), and glutamate oxaloacetate transaminase (GOT). The hybrid plants had an intermediate morphology, and grew vigorously in vitro. When transplanted to soil, they were less vigorous, due to difficulties in rooting, but were still capable of flowering, and forming short stolons and mishaped tubers, probably resulting from the effects of gene dosage due to the novel association of two genomes from a tuberizing (potato) and a non tuberizing species (L. pennellii). The characteristics of such mishaped tubers provided strong evidence of a hybrid nature for the selected plants. The hybrid plants were highly sterile, producing only 3-7% viable pollen. Tests for salt tolerance showed that the growth of the somatic hybrid plants was reduced by 50% as for L. penneUii, whilst potato did not grow at all under saline conditions.

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Electrofusion for the production of somatic hybrid plants of Solanum melongena L. and Solanum khasianum C.B. Clark

Cited by 67 publications

References 23 publications

Transfer of PLRV resistance fromSolanum verrucosum Schlechdt to potato (S. tuberosum L.) by protoplast electrofusion

Transfer of PLRV resistance fromSolanum verrucosum Schlechdt to potato (S. tuberosum L.) by protoplast electrofusion

The potential of somatic hybridization in crop breeding

Production and characterization of intergeneric somatic hybrids through protoplast electrofusion between potato (Solanum tuberosum) and Lycopersicon pennellii

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