Two new quinolines derivatives where crystallized from the acetylation reaction in low temperature ; between Baylis-Hillman products which had themselves been prepared from the Meth Cohn method and catalyzed by 1,4-diazabicyclo (Fatiha et al. in J Chem Crystallogr 42:989-996, 2012) octane and silicon dioxide or in pyridine and chloroform. Chemical structures have been established by spectral techniques of FTIR, 1H NMR and X-ray single crystal in low temperature. The crystal structure of (1a) crystallizes in triclinic space group P-1, a = 7.9145 (12) Å , b = 9.1412(11) Å , c = 10.7286(16) Å , a = 94.760(11)°, b = 105.988(13)°, c = 101.818(11)°, while (2a) crystallizes in orthorhombic non-centrosymmetric space group Pna21, a = 7.7237(12) Å , b = 20.2539(28) Å , c = 8.7164(12) Å , and its cohesion was found to be assured by O-HÁÁÁO and C-HÁÁÁO hydrogen bonds. Antimicrobial activity (in vitro) was evaluated by Gram-positive and Gram-negative bacteria. The compounds have shown good biological activity with Grampositive bacteria (Staphylococcus aurous and Staphylococcus coagulase-negative).Graphical Abstract Two new Quinoline derivatives have been prepared with good products yields; these products constitute original compounds, of great interest biological activity.