2003
DOI: 10.1078/0176-1617-00833
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Electrolyte leakage and lipid degradation account for cold sensitivity in leaves ofCoffea sp. plants

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Cited by 450 publications
(268 citation statements)
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“…Regarding cold stress damage in plants, some evaluations of oxidative damage has been performed (Kim & Tai 2011, Zhang et al 2011, Kim et al 2012. Oxidative damage can be indirectly evaluated by malondialdehyde content measurement, which is considered as a marker of cold damage (Campos et al 2003, Kim & Tai 2011. Another widely used tool to measure photosynthetic activity and stress signal in plants is chlorophyll fluorescence (Sikuku et al 2010).…”
Section: Introductionmentioning
confidence: 99%
“…Regarding cold stress damage in plants, some evaluations of oxidative damage has been performed (Kim & Tai 2011, Zhang et al 2011, Kim et al 2012. Oxidative damage can be indirectly evaluated by malondialdehyde content measurement, which is considered as a marker of cold damage (Campos et al 2003, Kim & Tai 2011. Another widely used tool to measure photosynthetic activity and stress signal in plants is chlorophyll fluorescence (Sikuku et al 2010).…”
Section: Introductionmentioning
confidence: 99%
“…After saponification, fatty acids were methylated with BF 3 (Merck) according to Mercalfe et al [14] using heptadecanoic acid (C17:0) as an internal standard. Subsequently they were analysed by gas-liquid chromatography as described in Mercalfe et al [22]. …”
Section: Lipid Analysismentioning
confidence: 99%
“…For lipid analysis, the general procedure of Pham Thi et al [20] was used with modification according to Scotti Campos et al [22]. Lipids were extracted in chloroform/methanol/water (1/1/1, v/v/v) according to Allen et al [1].…”
Section: Lipid Analysismentioning
confidence: 99%
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“…Cold temperatures reduce enzymatic activity, alter metabolism, and decrease photosynthetic capacity of plant tissues (Dubey, 1997). These changes are often associated with increased membrane permeability and degradation of membrane integrity (Campos et al, 2003), causing electrolytes from stressed cells to leak into the surrounding apoplast. EL estimates cell damage and hardiness by comparing conductivity of leaked contents from injured and uninjured tissues (Mattsson, 1996;McKay, 1992).…”
Section: Introductionmentioning
confidence: 99%