Electrolytes and nitrogen compounds of body fluids and tissues ofOctopus vulgaris Lam.

D’Aniello, Antimo; Strazzullo, L; D'Onofrio, Guiseppe; Pischetola, M.

doi:10.1007/bf00691036

Cited by 21 publications

(18 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The perfusion solution (PS) contained filtered sea water containing 2.78 mM anhydrous glucose, and was gassed with 99.5% oxygen and 0.5% carbon dioxide. The pH of the perfusion medium was 8.0 (as in: Houlihan et al, 1987; Agnisola et al, 1989; Agnisola and Houlihan, 1994) and is close to the pH of O. vulgaris haemolymph (pH = 7.8 ± 0.1; D'Aniello et al, 1986). …”

Section: Methodsmentioning

confidence: 80%

Effect of Different Formulations of Magnesium Chloride Used As Anesthetic Agents on the Performance of the Isolated Heart of Octopus vulgaris

et al. 2016

View full text Add to dashboard Cite

Magnesium chloride (MgCl2) is commonly used as a general anesthetic in cephalopods, but its physiological effects including those at cardiac level are not well-characterized. We used an in vitro isolated perfused systemic heart preparation from the common octopus, Octopus vulgaris, to investigate: (a) if in vivo exposure to MgCl2 formulations had an effect on cardiac function in vitro and, if so, could this impact recovery and (b) direct effects of MgCl2 formulations on cardiac function. In vitro hearts removed from animals exposed in vivo to 3.5% MgCl2 in sea water (20 min) or to a mixture of MgCl2+ ethanol (1.12/1%; 20 min) showed cardiac function (heart rate, stroke volume, cardiac output) comparable to hearts removed from animals killed under hypothermia. However, 3.5% MgCl2 (1:1, sea water: distilled water, 20 min) produced a significant impairment of the Frank-Starling response as did 45 min exposure to the MgCl2+ ethanol mixture. Perfusion of the isolated heart with MgCl2± ethanol formulations produced a concentration-related bradycardia (and arrest), a decreased stroke volume and cardiac output indicating a direct effect on the heart. The cardiac effects of MgCl2 are discussed in relation to the involvement of magnesium, sodium, chloride, and calcium ions, exposure time and osmolality of the formulations and the implications for the use of various formulations of MgCl2 as anesthetics in octopus. Overall, provided that the in vivo exposure to 3.5% MgCl2 in sea water or to a mixture of MgCl2+ ethanol is limited to ~20 min, residual effects on cardiac function are unlikely to impact post-anesthetic recovery.

show abstract

Section: Methodsmentioning

confidence: 80%

Effect of Different Formulations of Magnesium Chloride Used As Anesthetic Agents on the Performance of the Isolated Heart of Octopus vulgaris

et al. 2016

View full text Add to dashboard Cite

show abstract

“…On the one hand, the fluorescence labeling method with 5-(6)-carboxyfluorescein-N-hydroxysuccinimide ester has an advantage over fluorescein isothiocyanate (FITC) in that it allows an effective labeling at pH 7.4 and in that the amide bond formed after the reaction is more stable than the thio-urea bond formed between FITC and free amino groups. As a consequence, unwanted binding of pure non-inactivated fluorescein during the control injection into the circulatory system could not be ruled out, i.e., it is quite likely that autolabeling reactions between fluorescein molecules and mainly hemocyanin take place, because the native hemolymph has a pH of 7.4 and a hemocyanin content of about 80% (D'Aniello et al 1986;Senozan et al 1988;). Finally, there were no experimental differences between the injection of the fluorescein-labeled Sepia hemocyanin and the pure fluorescein, i.e., as for the gold-labeled Sepia hemocyanin, the conjugated fluorescein molecules mask the receptor-binding sites and therefore no fluoresceinlabeled Sepia hemocyanin occurred in the rhogocytes.…”

Section: In Situ Injection Of Fluorescein-labeled Sepia Hemocyaninmentioning

confidence: 98%

Hemocyanin and the branchial heart complex of Sepia officinalis : are the hemocytes involved in hemocyanin metabolism of coleoid cephalopods?

Beuerlein

Ruth

Westermann

et al. 2002

Cell and Tissue Research

View full text Add to dashboard Cite

Cytobiological experiments using isotopic- and cytochemical-labeled Sepia hemocyanin as well as immunocytochemical localization of the respiratory pigment were carried out to investigate the function of the hemocytes in hemocyanin metabolism of the common cuttlefish Sepia officinalis. For comparison, the rhogocytes (ovoid cells) of the branchial heart complex were included in this study. Hemocyanin molecules were immunocytochemically detected in the lysosomal compartment of the rhogocytes and, at lower levels, in adhesive and circulating hemocytes. (125)I-labeled Sepia hemocyanin was taken up by the rhogocytes only, whereas gold- and/or fluorescein-labeled Sepia hemocyanin was solely taken up by the adhesive and the circulating hemocytes, even though the level of uptake is different. There are also differences in the uptake of pure gold particles and/or fluorescein between rhogocytes and hemocytes. These findings give evidence that circulating and adhesive hemocytes of the branchial heart complex are not involved in hemocyanin turnover, but are a component of the cellular defense and detoxification system of adult coleoid cephalopods.

show abstract

“…Copper is an important component of respiratory pigment hemocyanin (Barceloux, 1999b). Indeed, 85% of the total copper in O. vulgaris is bound to this respiratory pigment (DÕAniello et al, 1986) and hemocyanin represents 98% of the blood proteins in cephalopods (Ghiretti, 1966). Zinc acts as a cofactor in a variety of cellular processes, including DNA synthesis (Barceloux, 1999c).…”

mentioning

confidence: 99%

Interannual patterns of variation in concentrations of trace elements in arms of Octopus vulgaris

Seixas

Bustamante²,

Pierce³

2005

Chemosphere

View full text Add to dashboard Cite

Concentrations of essential (copper, iron, manganese, selenium, and zinc) and non-essential (arsenic, cadmium, mercury, and lead) trace elements were measured in arms of Octopus vulgaris. The cephalopods were sampled from commercial fishery landings at two sites on the Portuguese coast in spring 2002 and 2003. Mercury was determined using an Advanced Mercury Analyser Spectrophotometer (AMAS) and other trace elements were measured using inductively coupled plasma-atomic emission spectrometry (ICP-AES). Since high levels of arsenic were detected, identification of the forms present was carried out using high performance liquid chromatography (HPLC) followed by ICP-MS.Mean concentrations of trace elements analysed were in the following order: As > Zn > Fe > Cu ) Cd > Pb > Se > Mn ) Hg. The results of speciation of arsenic demonstrate that virtually all arsenic was in the arsenobetaine form, which is the less toxic form. The concentrations of several trace elements were generally high in samples from Viana in 2002. Cadmium concentrations were above the legal limit for human consumption in samples from Viana in 2002 and two of these animals also had lead concentrations that exceeded legal limits. Mercury appeared in all samples but levels were within legally defined safe limits. No relationship was detected between trace element concentrations and size or maturity of octopus.

show abstract

Electrolytes and nitrogen compounds of body fluids and tissues ofOctopus vulgaris Lam.

Cited by 21 publications

References 32 publications

Effect of Different Formulations of Magnesium Chloride Used As Anesthetic Agents on the Performance of the Isolated Heart of Octopus vulgaris

Effect of Different Formulations of Magnesium Chloride Used As Anesthetic Agents on the Performance of the Isolated Heart of Octopus vulgaris

Hemocyanin and the branchial heart complex of Sepia officinalis : are the hemocytes involved in hemocyanin metabolism of coleoid cephalopods?

Interannual patterns of variation in concentrations of trace elements in arms of Octopus vulgaris

Contact Info

Product

Resources

About