Electrolytic Properties of Solutions of Paraffin-chain Quaternary Ammonium Salts

Scott, Anne; Tartar, H. V.

doi:10.1021/ja01244a053

Cited by 118 publications

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“…Since only the CMC value 17 could be found for OTAB, the micelle weight and the aggregation number of OTAB were estimated to be in the range 4500-6500 u and 22-24, respectively, with the aid of the data 17,18 obtained for other surfactants of this series, illustrated in Fig. 4.…”

Section: Resultsmentioning

confidence: 99%

The possibility of the direct measurement of micelle weight by electrospray ionization mass spectrometry

Nohara

Ohkoshi

Sakai

1998

Rapid Commun. Mass Spectrom.

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Measurement of the molecular weight of micelles, which are characteristic aggregates of molecules, was conducted using electrospray ionization mass spectrometry (ESI-MS). Micelle solutions were prepared by dissolving octyltrimethylammonium bromide [OTAB: C 8 H 17 N(CH 3 ) 3 Br] in water alone, without other additives. From the measurement of the m/z of the doubly or triply charged ions, it was revealed that the micelle of OTAB exhibited an almost symmetrical and polydisperse distribution about the mean molecular weight of 5000-5500. This molecular weight information is in good accordance with other estimated values. This result demonstrated the possibility of a direct measurement of micelle weight by mass spectrometry. # 1998 John Wiley & Sons, Ltd. Received 17 August 1998; Revised 5 October 1998; Accepted 7 October 1998 Recently, molecular aggregates associated by noncovalent interactions have been actively studied regarding their function, structure, and the interaction associating them. Electrospray ionization (ESI)1,2 has made it possible to directly measure the molecular weight of biopolymer accurately by producing multiply charged ions from the solution phase.3,4 Furthermore, molecular aggregates 5 due to noncovalent interactions can be detected under the prevailing conditions of ESI because it is a soft ionization technique. A variety of investigations using ESI have reported aggregates such as protein-ligand, 6-9 enzymesubstrate or enzyme-inhibitor, 10,11 protein-protein, protein-peptide, or peptide-peptide 12-15 and antibioticsligand. 16 Various methods have been employed in the measurement of the molecular weight of the micelle that is produced by these characteristic aggregations of relatively small molecules, e.g., light-scattering spectrometry, gel-filtration liquid chromatography and viscosity measurement. These can offer the mean molecular weight and the weight distribution, calculated on the basis of an hypothesis or the rough molecular weight by adaptation of a suitable calibration curve prepared from standard samples. This communication demonstrates the possibility of the measurement of micelle weight by ESI-MS. The successful application of the mass spectrometer to the direct measurement of micelle weight may open up a new field in the research of molecular aggregates. EXPERIMENTAL MaterialsThe micelle solution was prepared by dissolving octyltrimethylammonium bromide (OTAB) in distilled deionized water, without any additives. The concentration of OTAB was 0.56 M which was four times the critical micelle concentration (CMC) of this surfactant. 17 Mass spectrometryThe mass spectrometer used was of sector type (JMS-AX505HA, JEOL, Tokyo) equipped with an ESI ion source (Analytica of Branford, Branford, CA). The ionization voltage of 3.5 kV was applied to the outlet of the stainless steel nozzle. The micelle solution was infused by a syringe pump at the constant flow rate of 1 mL min À1 . RESULTS AND DISCUSSIONThe change in bias imposed between capillary and skimmer (DCS), in the ion source, can...

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Section: Resultsmentioning

confidence: 99%

The possibility of the direct measurement of micelle weight by electrospray ionization mass spectrometry

Nohara

Ohkoshi

Sakai

1998

Rapid Commun. Mass Spectrom.

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“…Therefore, the application of the linear form of Eq. [3] is justified only in the regions close to the CMC. The estimated error in the CMC values determined in this way depends mainly on NaBr concentration and it is ca.…”

Section: Determination Of the Cmcmentioning

confidence: 99%

“…Among alkyltrimethylammonium bromides (C N TAB) the effect of temperature on micelle formation in aqueous solutions have been investigated for the octyl (3,4), nonyl (5,6), decyl (4)(5)(6)(7)(8)(9)(10)(11), dodecyl (3,4,(7)(8)(9)(10)(12)(13)(14)(15), tetradecyl (4,9,10,(18)(19)(20), and hexadecyl (3, 9, 10) derivatives. There have been only few publications (10,(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33) on the effect of added salt on the micelle formation in aqueous salt solutions of alkyltrimethylammonium bromides.…”

Section: Introductionmentioning

confidence: 99%

Effect of Temperature on Micelle Formation in Aqueous NaBr Solutions of Octyltrimethylammonium Bromide

Zieliński¹

2001

Journal of Colloid and Interface Science

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“…In this study, the consequences of incorporation of both cationic and anionic surfactants into phosphatidylcholine liposomes for phospholipase A-and C-promoted hydrolysis have been probed. (Scott & Tartar, 1943). U~s0nicated liposomes were prepared for kinetic work by a slight modification of the previous procedure (Jain & Cordes, 1973 a).…”

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Phospholipases. III. Effects of ionic surfactants on the phospholipase-catalyzed hydrolysis of unsonicated egg lecithin liposomes

1975

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Apparent values of Km and Vmax have been measured for catalysis of hydrolysis of unsonicated egg lecithin liposomes, activated through addition of 0.4 M n-hexanol, by phospholipases A2 from bee and snake venoms and by phospholipase C from Clostridium welchii as a function of the concentration of three surfactants: hexadecylamine, hexadecyltrimethylammonium bromide, and dihexadecyl phosphate. For all three enzymes, values of Km and Vmax show little or no dependence on the concentration of these ionic surfactants, demonstrating that the liposomal surface charge is not a crucial factor in determining susceptibility to phospholipase-catalyzed hydrolysis.

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Electrolytic Properties of Solutions of Paraffin-chain Quaternary Ammonium Salts

Cited by 118 publications

References 0 publications

The possibility of the direct measurement of micelle weight by electrospray ionization mass spectrometry

The possibility of the direct measurement of micelle weight by electrospray ionization mass spectrometry

Effect of Temperature on Micelle Formation in Aqueous NaBr Solutions of Octyltrimethylammonium Bromide

Phospholipases. III. Effects of ionic surfactants on the phospholipase-catalyzed hydrolysis of unsonicated egg lecithin liposomes

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