Electromagnetic fields promote osteogenesis of rat mesenchymal stem cells through the PKA and ERK1/2 pathways

Yong, Yang; Ming, Zhao; Li, Feng; Chun, Zhao; Wu, Hua

doi:10.1002/term.1864

Cited by 46 publications

(40 citation statements)

References 32 publications

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“…Ke et al found EMF exposure could cause the cluster- ing of epidermal growth factor (EGF) receptors and RAS activation (Ke et al, 2008). What is more, many researchers demonstrated that expression of phosphorylated ERK1/2 and P38 of BMSCs or osteoblasts could be promoted by EMF exposure, and the MAPK signaling pathway special inhibitor could inhibit the effect of EMFs on promoting osteogenic differentiation (Song et al, 2014a;Yong et al, 2014;Yuge et al, 2003). In conclusion, the RAS/RAF/MAPK signaling pathway may be another possible signaling pathway involving the biological effects of EMFs (Figure 1).…”

Section: Ras/raf/mapk Signaling Pathwaymentioning

confidence: 97%

Effects of electromagnetic fields on osteoporosis: A systematic literature review

Wang¹,

Yong

et al. 2016

Electromagnetic Biology and Medicine

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Electromagnetic fields (EMFs) as a safe, effective and noninvasive treatment have been researched and used for many years in orthopedics, and the common use clinically is to promote fracture healing. The effects of EMFs on osteoporosis have not been well concerned. The balance between osteoblast and osteoclast activity as well as the balance between osteogenic differentiation and adipogenic differentiation of bone marrow mesenchymal stem cells plays an important role in the process of osteoporosis. A number of recent reports suggest that EMFs have a positive impact on the balances. In this review, we discuss the recent advances of EMFs in the treatment of osteoporosis from basic research to clinical study and introduce the possible mechanism. In addition, we presented future perspectives of application of EMFs for osteoporosis.

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Section: Ras/raf/mapk Signaling Pathwaymentioning

confidence: 97%

Effects of electromagnetic fields on osteoporosis: A systematic literature review

Wang¹,

Yong

et al. 2016

Electromagnetic Biology and Medicine

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“…Hou et al (2006) indicated that EMF activated the extracellular signal-regulated kinase mitogen-activated protein kinase (ERK–MAPK) and p38 pathways [27]. In a study by Yong et al (2014), it was demonstrated that 15 Hz, 1 mT promoted mesenchymal stem cells (MSCs) osteogenesis and that the EMF-induced osteogenic markers were mediated by both the PKA and MAPK signaling pathways [28]. In fact, EMFs activate some of the signaling pathways.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of osteogenic differentiation of rat adipose tissue-derived mesenchymal stem cells by zinc sulphate under electromagnetic field via the PKA, ERK1/2 and Wnt/β-catenin signaling pathways

Fathi

Farahzadi

2017

PLoS ONE

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Zinc ion as an essential trace element and electromagnetic fields (EMFs) has been reported to be involved in the regulation of bone metabolism. The aim of this study was to elucidate the effects of zinc sulphate (ZnSO4) on the osteogenic differentiation of adipose tissue-derived mesenchymal stem cells (ADSCs) in the presence of EMF as a strategy in osteoporosis therapy. Alkaline phophatase (ALP) activity measurement, calcium assay and expression of several osteoblastic marker genes were examined to assess the effect of ZnSO4 on the osteogenic differentiation of ADSCs under EMF. The expression of cAMP and PKA was evaluated by ELISA. The expression of β-catenin, Wnt1, Wnt3a, low-density lipoprotein receptor-related protein 5 (LRP5) and reduced dickkopf1 (DKK1) genes were used to detect the Wnt/β-catenin pathway. It was found that ZnSO4, in the presence of EMF, resulted in an increase in the expression of osteogenic genes, ALP activity and calcium levels. EMF, in the presence of ZnSO4, increased the cAMP level and protein kinase A (PKA) activity. Treatment of ADSCs with (MAPK)/ERK kinase 1/2 inhibitor, or PKA inhibitor, significantly inhibited the promotion of osteogenic markers, indicating that the induction of osteogenesis was dependent on the ERK and PKA signaling pathways. Real-time PCR analysis showed that ZnSO4, in the presence of EMF, increased the mRNA expressions of β-catenin, Wnt1, Wnt3a, LRP5 and DKK1. In this study, it was shown that 0.432 μg/ml ZnSO4, in the presence of 50 Hz, 20 mT EMF, induced the osteogenic differentiation of ADSCs via PKA, ERK1/2 and Wnt/β-catenin signaling pathways.

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“…Therefore, the improvement of the present available technologies is still needed to acquire more satisfactory clinical outcomes in bone defect repair. PEMFs, as described above, have a marked function to accelerate the proliferation, osteogenic differentiation, and mutation of BMSCs by activating a series of signaling pathways [7, 21, 25, 31, 38, 73]. Moreover, the expressions of many osteogenesis- and angiogenesis-promoting cytokines, including TGF-β, BMPs, IGFs, FGFs, and VEGFs, in BMSCs are strikingly elevated by PEMF exposure.…”

Section: Therapeutic Applications Of Pemfs In Bone Repairmentioning

confidence: 99%

“…When the cells were treated with U0126, an inhibitor of the ERK1/2 signaling cascade, the positive effects of the ELF-PEMF treatment on osteoblast function were abolished [36]. Other studies also revealed that the MEK/ERK signaling pathway regulated the promoting effects of PEMF on bone marrow mesenchymal stem cell (BMSC) proliferation, expression of osteogenic genes (RUNX2, BSP, OPN), ALP activity, and calcium deposition [22, 32, 37, 38]. Additionally, one study reported that the p38 MAPK pathway is involved in the increased production of collagen synthesis in osteoblast-like cells stimulated by ELF-EMF exposure [39].…”

Section: Underlying Signaling Pathwaysmentioning

confidence: 99%

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Underlying Signaling Pathways and Therapeutic Applications of Pulsed Electromagnetic Fields in Bone Repair

Yuan

Xin

Jiang

2018

Cell Physiol Biochem

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Pulsed electromagnetic field (PEMF) stimulation, as a prospective, noninvasive, and safe physical therapy strategy to accelerate bone repair has received tremendous attention in recent decades. Physical PEMF stimulation initiates the signaling cascades, which effectively promote osteogenesis and angiogenesis in an orchestrated spatiotemporal manner and ultimately enhance the self-repair capability of bone tissues. Considerable research progresses have been made in exploring the underlying cellular and subcellular mechanisms of PEMF promotion effect in bone repair. Moreover, the promotion effect has shown strikingly positive benefits in the treatment of various skeletal diseases. However, many preclinical and clinical efficacy evaluation studies are still needed to make PEMFs more effective and extensive in clinical application. In this review, we briefly introduce the basic knowledge of PEMFs on bone repair, systematically elaborate several key signaling pathways involved in PEMFs-induced bone repair, and then discuss the therapeutic applications of PEMFs alone or in combination with other available therapies in bone repair, and evaluate the treatment effect by analyzing and summarizing recent literature.

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Electromagnetic fields promote osteogenesis of rat mesenchymal stem cells through the PKA and ERK1/2 pathways

Cited by 46 publications

References 32 publications

Effects of electromagnetic fields on osteoporosis: A systematic literature review

Effects of electromagnetic fields on osteoporosis: A systematic literature review

Enhancement of osteogenic differentiation of rat adipose tissue-derived mesenchymal stem cells by zinc sulphate under electromagnetic field via the PKA, ERK1/2 and Wnt/β-catenin signaling pathways

Underlying Signaling Pathways and Therapeutic Applications of Pulsed Electromagnetic Fields in Bone Repair

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