The quantum fluctuations of the geodesic deviation equation in a flat background spacetime are discussed. We calculate the resulting mean squared fluctuations in the relative velocity and separation of test particles. The effect of these quantum fluctuations of the spacetime geometry is given in terms of the Riemann tensor correlation function. Three different sources of the Riemann tensor fluctuations are considered: a thermal bath of gravitons, gravitons in a squeezed state, and the graviton vacuum state. * Horacio.Vieira@tufts.edu and On the other hand, the investigation of the Brownian motion, which can be described by the Langevin equation, played a very important role for the establishment of the atomic structure of matter. The discreteness character of matter (microscopic feature) causes fluctuations in the density of matter, which, in turn, causes observable effects on the motion of the Brownian particle (macroscopic feature) [16]. Recently, the solutions of Langevin-type equations in some astrophysical scenarios have been discussed in the literature [17][18][19][20][21][22][23].The knowledge of the behavior of a Brownian particle immersed in a fluid of much smaller atoms, can give us, in principle, some relevant information about the physics of these objects [24]. Brownian motion of test particles coupled to quantized fields was studied in Refs. [25][26][27]. Similarly, we can study the Brownian motion of test particles in a fluctuating gravitational field to look for insights into quantum gravity [28] . In this way, we will use the geodesic deviation equation as a Langevin equation in which the Riemann tensor fluctuates.These quantum fluctuations of the curvature modify the motion of test particles and can be measured by the relative velocity dispersion after an interaction.The quantum fluctuations of the spacetime geometry can be of two types: passive and active. The passive case is generated by fluctuations of the quantum matter fields, that is,