Electromagnetic scattering in a discrete basis

Trampel, Christopher

doi:10.31274/etd-180810-88

Cited by 1 publication

(2 citation statements)

References 33 publications

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“…End-on attachment of densely packed perpendicularly orientated spirochaetes to the apical surface of the colonic epithelium like a false brush border has been reported for S. pilosicolin infections in pigs (13,26). Similar end-on attachment of intestinal spirochaetes to colonic epithelium is well known in other domestic animals as well as in humans (25,27). In humans affected with chronic diarrhoea the spirochaete Brachyspira aalborgi can be seen end-on attached to epithelial cells involving substantial areas of the colonic mucosa, although pathomorphological alterations are minimal and consist of shortening of microvilli adjacent to spirochaetes compared to microvilli in regions devoid of spirochaetes.…”

Section: Discussionsupporting

confidence: 54%

“…Thus, the extension of S. hyodysenteriae end-on attachment to the epithelium remains to be determined. Extensive end-on attachment of other intestinal spirochaetes to the colonic epithelium has been observed in pigs and other animals as well as in humans (8, 13,25,26,27). Detection of spirochaetes in tissue samples using different methods, including direct microscopy and e.g.…”

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confidence: 99%

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Association of Serpulina hyodysenteriae with the colonic mucosa in experimental swine dysentery studied by fluorescent in situ hybridization

Jensen¹,

Boye²,

Möller³

et al. 1998

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Association of Serpulina hyodysenteriae with the colonic mucosa in experimental swine dysentery studied by fluorescent in situ hybridization.The localization of Serpulina hyodysenteriae in experimental swine dysentery was studied by fluorescent in situ hybridization (FISH) using an oligonucleotide probe targeting the 23s rRNA of S. hyodysenteriae. Nine 8-week-old pigs were challenged. Seven of the pigs were intragastrically dosed with 1 X lo9 cfu S. hyodysenteriae for 3 consecutive days, whereas two pigs were infected by contact. Six non-challenged pigs served as negative controls. The challenged pigs developed clinical swine dysentery from 8 to 14 days postinfection with typical gross lesions. By FISH S. hyodysenteriae cells in huge numbers were found colonizing the mucus layer, the luminal epithelium and the crypts of the large intestinal mucosa. The orientation of the spirochaetes to the epithelium appeared to be random. Spirochaetes in clusters or as single cells were invading the epithelium and were occasionally detected in the adjacent lamina propria. The distribution of spirochaetes in the mucosa provides further evidence that S. hyodysenteriae is intimately associated with the mucus layer and the epithelium in a random pattern. Furthermore, the results demonstrate the applicability of FISH for specific detection of S. hyodysenteriae cells in clusters or as single cells in formalin-fixed tissue samples.

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