Electromagnetophoretic Force Measurement of a Single Binding Interaction between Lectin and Yeast Cell Surfaces

Iiguni, Yoshinori; Watarai, Hitoshi

doi:10.2116/analsci.23.121

Cited by 18 publications

(11 citation statements)

References 25 publications

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“…The apparent conductivities of polystyrene particles and yeast cells in 1 M KCl solution were 0.019 and 0.075 S cm -1 , respectively. 27,30 The separation medium was 0.155 M KCl solution, which had an ionic strength nearly equal to an isotonic solution used for the study of biological cells, containing 20% urea in order to match the densities of the polystyrene particles and the solution to prevent the sedimentation of the particles. Moreover, the flow rate was increased by three times for increasing the throughput; the flow rate from inlet 1 and 2 were 3.99 and 16.01 μL h -1 , respectively.…”

Section: Separation Between Heterogeneous Particles By Microchip Elecmentioning

confidence: 99%

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Continuous-flow Size-based Separation of Microparticles by Microchip Electromagnetophoresis

et al. 2015

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A novel microchip separation method for microparticles based on electromagnetophoresis was developed. In this method, a double Y-shape microchip with two inlets and two outlets was used for particle separation. Microparticles of two different sizes were suspended in an aqueous solution and introduced into the microchannel from one inlet with a stream of the aqueous solution without particles from another inlet by a hydrodynamic flow. Then, the particles with two different sizes were migrated orthogonal to the flow by elctromagnetophoresis and separated to different outlets based on the difference in their electromagnetophoric velocities, which depended on the size of particle. Using this method, at one outlet, 89% of 3 μm polystyrene latex particles were isolated from a mixture of 3 and 10 μm particles. By combining hydrodynamic focusing, both 3 and 10 μm particles were recovered at the different outlets with 100% purity and recovery, respectively. Even the separation of 3 and 6 μm particles was also achieved with about 98% recovery and purity. Moreover, yeast cells and polystyrene particles could be separated with high separation efficiency by this technique.

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Section: Separation Between Heterogeneous Particles By Microchip Elecmentioning

confidence: 99%

“…[27][28][29] In the present study, we developed a new microchip separation method for microparticles utilizing EMP. For the microchip EMP, electrodes to apply the electric current can be placed outside of the microchip, and the magnetic field is produced easily by a permanent Nd-Fe-B magnet located under the microchip.…”

Section: Introductionmentioning

confidence: 99%

Continuous-flow Size-based Separation of Microparticles by Microchip Electromagnetophoresis

et al. 2015

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“…[4][5][6] Furthermore, an electromagnetophoretic force was applied to dynamic force measurements of chemical interactions between a particle and a capillary wall and a dynamic force analysis of the breaking of interactions between a yeast cell and lectin on the wall of a capillary. [7][8][9][10] The advantages of using an electromagnetophoretic force for the separation of particles and the dynamic force measurement are that: (i) a non-contact migration of particles is possible, (ii) an open solution system is acceptable, and (iii) the separation force is easily controllable by an electric current. Usually, the electromagnetophoretic (EMP) force, FEMP, is represented by a simplified equation assuming a uniform electric current and a uniform magnetic field that are perpendicularly applied on a particle in an electrolyte solution, …”

Section: Introductionmentioning

confidence: 99%

Electromagnetophoretic Micro-convection around a Droplet in a Capillary

2017

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The electromagnetophoretic behavior of organic droplets in an electrolyte solution was investigated in a silica capillary cell using a superconducting bulk magnet (3.5 T) and a magnetic circuit (2.7 T). The initially dispersed emulsion droplets of dodecane migrated to the wall of the capillary, responding to the direction of an electric current, and coalesced to form smaller and larger droplets after some repeated migrations. When the electric current was applied continuously, the larger droplets became arranged with regular intervals on the wall, and smaller droplets rotated around the larger droplets. These interesting behaviors were analyzed while taking into account the local electric current density determined by the flow velocity of the ionic current around a droplet, which was lowest on the electrode sides of the droplet. The difference in the local electric current density generated the Lorentz-force difference in the medium, which lead to local microconvection around the droplet, and also the alignment of larger droplets by a repelling effect between the adjacent microconvections.

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“…Laser photophoresis [11][12][13][14][15][16], driven by the radiation pressure of photons, allows us to estimate the reflectance, refractive index, and absorption coefficient and other optical properties of microparticles, and to separate them by utilizing the difference in such properties. Electromagnetophoresis [17][18][19][20][21][22], which is migration under a homogeneous magnetic field and an electric current, has great potential not only for characterizing microparticles by conductivity measurement, but also for measuring the adhesion force between microparticles in a liquid and the cell wall by measuring desorption current. Magnetophoresis [23][24][25][26][27][28][29][30] can be observed under an inhomogeneous magnetic field.…”

Section: Introductionmentioning

confidence: 99%

Magnetic susceptibility measurement of single iron/cobalt carbonyl microcrystal by atmospheric magnetophoresis

Suwa

Oshino

Watarai

et al. 2008

Science and Technology of Advanced Materials

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In this study, the use of an innovative atmospheric magnetophoresis, which enables us to measure the mass magnetic susceptibility and mass of a microparticle simultaneously, was demonstrated. Using this technique, we determined the magnetic susceptibility of a crystalline deposit of iron/cobalt carbonyl, mainly composed of Fe 2 (CO) 9 , which was prepared photochemically from a gaseous mixture of iron pentacarbonyl (Fe(CO) 5 ) and cobalt tricarbonyl nitrosyl (Co(CO) 3 NO). The mass magnetic susceptibility and the characteristic relaxation time of the microcrystal were (7.0 ± 1.9) × 10 −9 m 3 kg −1 and (5.6 ± 2.2) × 10 −4 s, respectively. The observed magnetic susceptibility shows that the microparticle was paramagnetic. Assuming that the density was equal to that of Fe 2 (CO) 9 (2.1 × 10 3 kg m −3 ) and that the shape of the particle was spherical, a hydrodynamic radius of 4.7 µm and a mass of 0.91 ng were observed. It was suggested that Co was incorporated in Fe 2 (CO) 9 .

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Electromagnetophoretic Force Measurement of a Single Binding Interaction between Lectin and Yeast Cell Surfaces

Cited by 18 publications

References 25 publications

Continuous-flow Size-based Separation of Microparticles by Microchip Electromagnetophoresis

Continuous-flow Size-based Separation of Microparticles by Microchip Electromagnetophoresis

Electromagnetophoretic Micro-convection around a Droplet in a Capillary

Magnetic susceptibility measurement of single iron/cobalt carbonyl microcrystal by atmospheric magnetophoresis

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