Electron and Optical Microscopy

Ben‐Dor, L.

doi:10.1016/b978-0-08-028670-9.50026-0

Advances in Cement Technology 1983

DOI: 10.1016/b978-0-08-028670-9.50026-0

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Electron and Optical Microscopy

L. Ben‐Dor

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1993

2005

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Cited by 2 publications

References 249 publications

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GuaB Activity Is Required in Rhizobium tropici During the Early Stages of Nodulation of Determinate Nodules but Is Dispensable for the Sinorhizobium meliloti-Alfalfa Symbiotic Interaction

Collavino¹,

Riccillo²,

Grasso³

et al. 2005

MPMI

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The guaB mutant strain Rhizobium tropici CIAT8999-10T is defective in symbiosis with common bean, forming nodules that lack rhizobial content. In order to investigate the timing of the guaB requirement during the nodule formation on the host common bean by the strain CIAT899-10.T, we constructed gene fusions in which the guaB gene is expressed under the control of the symbiotic promoters nodA, bacA, and nifH. Our data indicated that the guaB is required from the early stages of nodulation because full recovery of the wild-type phenotype was accomplished by the nodA-guaB fusion. In addition, we have constructed a guaB mutant derived from Sinorhizobium meliloti 1021, and shown that, unlike R. tropici, the guaB S. meliloti mutant is auxotrophic for guanine and induces wild-type nodules on alfalfa and Medicago truncatula. The guaB R. tropici mutant also is defective in its symbiosis with Macroptilium atropurpureum and Vigna unguiculata but normal with Leucaena leucocephala. These results show that the requirement of the rhizobial guaB for symbiosis is found to be associated with host plants that form determinate type of nodules.

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GuaB Activity Is Required in Rhizobium tropici During the Early Stages of Nodulation of Determinate Nodules but Is Dispensable for the Sinorhizobium meliloti-Alfalfa Symbiotic Interaction

Collavino¹,

Riccillo²,

Grasso³

et al. 2005

MPMI

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Microstructure of Hardened Cement Paste—A New Interpretation

Diamond

Bonen

1993

Journal of the American Ceramic Society

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Morphological features of hydrated portland cement paste exhibited on carefully polished surfaces have been critically examined using backscatter mode scanning electron microscopy. A new interpretation and classification of such features is proposed. The microstructure is seen to consist of distinct grains of the order of 10 pm and larger ("phenograins") set in a cellular groundmass of smaller particles and pores. Most of the phenograins are dense pseudomorphs of the larger cement grains, but some are large calcium hydroxide crystals, and in some cements a few may represent inert filler grains added to the cement. The phenograins that are pseudomorphs of cement particles may be solid, gapped, or hollow; and, at a given age, various stages of hydration are represented in different grains. While many pseudomorph phenograins consist of a simple core of unhydrated material surrounded by a uniform shell of hydration product, more complex and irregular structures are also found. Relationships between these features and earlier classifications of cement hydration product particles are discussed, and implications with respect to difficulties in image analysis of hardened cement paste are examined.

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Electron and Optical Microscopy

Cited by 2 publications

References 249 publications

GuaB Activity Is Required in Rhizobium tropici During the Early Stages of Nodulation of Determinate Nodules but Is Dispensable for the Sinorhizobium meliloti-Alfalfa Symbiotic Interaction

GuaB Activity Is Required in Rhizobium tropici During the Early Stages of Nodulation of Determinate Nodules but Is Dispensable for the Sinorhizobium meliloti-Alfalfa Symbiotic Interaction

Microstructure of Hardened Cement Paste—A New Interpretation

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