Electron capture gas chromatographic determination of Kepone® residues in environmental samples

Moseman, Robert F.; Crist, Howard L.; Edgerton, Thomas R.; Ward, M. K.

doi:10.1007/bf02097763

Cited by 34 publications

(9 citation statements)

References 10 publications

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“…Figure 3 shows the LH-20 GPC elution profiles of these Kepone derivatives and [14C]Kepone hydrate. Moseman et al (1977) reported that the addition of methanol to hexane solutions of Kepone increased the sensitivity of Kepone to EC-GC analysis. Methanol increases the solubility of Kepone in hexane.…”

Section: Resultsmentioning

confidence: 99%

Fate of Kepone and mirex in the aquatic environment

Huckins¹,

Stalling²,

Petty³

et al. 1982

J. Agric. Food Chem.

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Section: Resultsmentioning

confidence: 99%

Fate of Kepone and mirex in the aquatic environment

Huckins¹,

Stalling²,

Petty³

et al. 1982

J. Agric. Food Chem.

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“…Chlordecone (decachlorooctahydro‐1,3,2‐metheno‐2 H ‐cyclobuta[ c,d ]pentalen‐2‐one) is an organo‐chlorine insecticide that was formerly used extensively 1. The compound is a member of the persistent organic pollutants that exhibit remarkably long half‐lives in the environment, and it is well known that chlordecone shows various toxicities such as arrest of sperm maturation,2 blocking of reproductive function,3 and a variety of estrogen‐like effects on the female reproductive system in birds and mammals 4.…”

mentioning

confidence: 99%

Detection of chlordecone by liquid chromatography with tandem mass spectrometry

Moriwaki

Hasegawa

2004

Rapid Comm Mass Spectrometry

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Detection of chlordecone by liquid chromatography with tandem mass spectrometryChlordecone (decachlorooctahydro-1,3, 2-metheno-2H-cyclobuta [c,d]pentalen-2-one) is an organo-chlorine insecticide that was formerly used extensively. 1The compound is a member of the persistent organic pollutants that exhibit remarkably long half-lives in the environment, and it is well known that chlordecone shows various toxicities such as arrest of sperm maturation, 2 blocking of reproductive function, 3 and a variety of estrogen-like effects on the female reproductive system in birds and mammals. 4 Therefore, it is very important to understand the chlordecone concentration in various environments.In general, chlordecone is determined by gas chromatography with electron capture detection (GC/ECD). 5 However, in order to apply an appropriate method to the analysis of chlordecone in various environmental media and matrices, it is important to develop a novel analytical method to complement the methods based on GC. We have developed an analytical method for chlordecone using liquid chromatography with tandem mass spectrometry (LC/MS/MS). The LC/ MS/MS technique has the potential to simplify the analytical pretreatment, and to provide rapid confirmation of chlordecone in environmental samples.Chlordecone was purchased from Wako Pure Chemical Industries (Osaka, Japan). A stock solution at a concentration of 1000 mg/mL was prepared by dissolution of chlordecone in methanol. The solutions were stored in the dark. Working standards were prepared on a daily basis by dilution of the stock solution with methanol. Chlordecone-13 C 8 , used as an internal standard, was obtained from Cambridge Isotope Laboratories Inc. (Andover, USA). Five mL of the internal methanol solution (0.1 mg/mL) was added to the chlordecone standard solutions (1.0 mL).The LC/MS/MS analysis was performed using a MDS-Sciex API 3000 system (Applied Biosystems). Ionization of chlordecone was achieved by electrospray (ESI-MS) in the negative ion mode. All the interface parameters were optimized by infusing a standard solution (0.1 mg/mL) of the analytes. The final electrospray conditions were as follows: nitrogen curtain gas, 6 psi; ion spray voltage, À4500 V; declustering potential, À81 V. The full-scan single-quadrupole mass spectra were obtained in the range m/z 400-700 at a scan rate of 1 scan/s. The product-ion mass spectrum of the ion (m/z 507) was acquired in the range m/z 10-520 at a scan rate of 1 scan/s. The collision energies were À28 and À32 eV for chlordecone and the internal standard, respectively. The LC/MS/MS acquisition was performed in the multiple reaction monitor (MRM) mode by following the reactions m/z 507 ! 427, characteristic of chlordecone, and m/z 515 ! 434, characteristic of the internal standard, chlordecone-13 C 8 . Liquid chromatography was conducted using an 1100 series (Agilent) separation module interfaced to the API 3000 system. A Develosil C30-UG-5 column (Nomura Chemicals; 5 mm particle size, 2.0 mm i.d. Â 150 mm) was used for the LC sepa...

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“…Numerous studies, dedicated to the CLD determination in various matrixes has been conducted since 1960. Electron capture gas chromatography (GC-ECD) has become a technique of choice for the determination of CLD in water [4] and [5], soil and sediments [5], [6] and [7] food [8] and biological tissues [9] and [10]. More recently gas chromatography equipped with mass spectrometry has also been employed for CLD determination (GC-MS).…”

Section: Introductionmentioning

confidence: 99%