Electron-Capture GLC Determination of Blood Levels of 7-Chloro-1,3-dihydro-5- (2'-chlorophenyl) -2H - 1,4-benzodiazepin-2-one in Humans and Its Urinary Excretion as Lorazepam Determined by Differential Pulse Polarography

Trace level determination of 1,4-benzodiazepines in blood by differential pulse polarography

“…The use of differential pulse polarography (DPP) to measure drugs in biological fluids has been reported (2). DPP has recently been applied to the determination of trimethoprim and its metabolites in blood and urine (2, 3), phenobarbital, diphenylhydantoin (4), several nitroimidazoles (5) and glibornuride in blood (6), and to the measurement of the urinary excretion of several 1,4benzodiazepines (7)(8)(9). Chlordiazepoxide and its metabolites in human plasma have been measured with an absolute limit of detection of 50-100 ng/2 ml plasma (20).…”