Electron holography of individual DNA molecules

Fink, Hans‐Werner; Schmid, Heinz; Ermantraut, Eugen; Schulz, Torsten

doi:10.1364/josaa.14.002168

Cited by 69 publications

(55 citation statements)

References 9 publications

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“…4,5 We have recently demonstrated that electrons with kinetic energies in the range of 50-200 eV do not cause radiation damage to biomolecules, enabling the investigation of an individual molecule for an extended period of time. 6 Since the electron wavelength associated with this kinetic energy range is between 0.86 Å (for 200 eV) and 1.7 Å (for 50 eV), low-energy electrons have the potential for nondestructive imaging of single biomolecules 7,8 and in particular individual proteins at atomic resolution.…”

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confidence: 99%

Non-destructive imaging of an individual protein

et al. 2012

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The mode of action of proteins is to a large extent given by their ability to adopt different conformations. This is why imaging single biomolecules at atomic resolution is one of the ultimate goals of biophysics and structural biology. The existing protein database has emerged from X-ray crystallography, NMR or cryo-TEM investigations. However, these tools all require averaging over a large number of proteins and thus over different conformations. This of course results in the loss of structural information. Likewise it has been shown that even the emergent X-FEL technique will not get away without averaging over a large quantity of molecules. Here we report the first recordings of a protein at sub-nanometer resolution obtained from one individual ferritin by means of low-energy electron holography. One single protein could be imaged for an extended period of time without any sign of radiation damage. Since ferritin exhibits an iron core, the holographic reconstructions could also be cross-validated against TEM images of the very same molecule by imaging the iron cluster inside the molecule while the protein shell is decomposed

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confidence: 99%

Non-destructive imaging of an individual protein

et al. 2012

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“…We previously reported that the pore diameter of MPS affects the protein and DNA adsorption capacities [18,21]. The diameter of the DNA double strand is approximately 2 nm [30], which may be related to the low DNA adsorption capacities observed in the present study. We prepared MCM-41c 12/1 with pores that were approximately twice as large as those of MCM-41c 12/1, and named it MCM-41c 12/1 5 nm.…”

Section: Pore Sizementioning

confidence: 51%

Elucidating the effect of different amino-functionalized spherical mesoporous silica characteristics on ribonucleic acid selectivity and adsorption capacity

Hikosaka

Nagata

Tomita

et al. 2018

Journal of Asian Ceramic Societies

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“…However, this technique has not been applied to the electron microscopy yet. Several works deal with the point-projection electron microscopy, [10][11][12] focusing on studying uncoated and unstained macromolecules.…”

Section: Ref 1))mentioning

confidence: 99%

“…10) A carbon film 3 nm thick, deposited on a gilded holey carbon foil placed on a copper grid, was investigated as the ultimate specimen. …”

Section: )mentioning

confidence: 99%

Very Low Energy Scanning Electron Microscopy of Free-Standing Ultrathin Films

et al. 2010

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Institute of Scientific Instruments of the ASCR, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic Instrument and methodology is presented for very low energy scanning transmission electron microscopy. The detector system provides simultaneous acquisitions of total reflected and transmitted electron fluxes. Introductory experiments incorporated examination of ultrathin foils of gold, carbon and graphene flakes. Extremely sensitive thickness contrast obtained at units of eV is demonstrated. The phenomenon of electron transmissivity apparently exceeding 100% owing to the contribution of secondary electrons released near to the exit surface is described and discussed.

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Electron holography of individual DNA molecules

Cited by 69 publications

References 9 publications

Non-destructive imaging of an individual protein

Non-destructive imaging of an individual protein

Elucidating the effect of different amino-functionalized spherical mesoporous silica characteristics on ribonucleic acid selectivity and adsorption capacity

Very Low Energy Scanning Electron Microscopy of Free-Standing Ultrathin Films

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