Electron Microscope Studies of Diclidophora merlangi (Monogenea: Polyopisthocotylea). I. Ultrastructure of the Cecal Epithelium

Halton, D W; Dermott, Evelyn; Morris, Gerald P.

doi:10.2307/3277118

Cited by 29 publications

(17 citation statements)

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“…The gastrodermis of A. heterodus is quite similar to the pattern described for other Polyopisthocotylea, with two cell types: hematin cell and connecting syncytium (Llewelyn 1954, Halton & Jennings 1965, Halton et al 1968, Tinsley 1973, Halton 1975.…”

Section: Discussionmentioning

confidence: 49%

“…Longitudinal sections showed that the irregular "U"-shaped hematin cells, are different from those previously reported for polyopisthocotyleans (Halton et al 1968, Fournier 1978, Allen & Tinsley 1989. The feeding process is continuous, with lamellae looping back onto the surface to capture and endocyte food particles, in a process similar to that described by Halton (1974), actually called micropinocytosis.…”

Section: Discussionmentioning

confidence: 57%

“…The structure of the syncytium cells with or without lamellae was previously reported (Halton et al 1968, Tinsley 1973. Nevertheless, in both cases these monogeneans that feed on blood present a syncytium with few spread organelles, functioning mainly as a support for the hematin cells, on the contrary to those that present a mixed food habit like Polystomoides sp., where the syncytial cytoplasm has numerous organelles (Allen & Tinsley 1989).…”

Section: Discussionmentioning

confidence: 86%

“…Ultrathin sections were picked up on uncoated 200-mesh copper grids, stained with uranyl acetate and lead citrate, and observed in a Zeiss 900 or Jeol JEM 100CX Electron Microscope. Some ultrathin sections were subjected to the picric acid/alcohol solubility test for hematin (Halton et al 1968). Control sections were incubated in acid-free alcohol.…”

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confidence: 99%

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The Ultrastructure of the Gastrodermis and the Nutrition of the Gill Parasitic Atriaster heterodus Lebedev and Paruchin, 1969 (Platyhelminthes: Monogenea)

Santos

Souto-Padrón

Lanfredi

1998

Mem. Inst. Oswaldo Cruz

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The gastrodermis of Atriaster heterodus Lebedev & Paruchin, 1969 (Polyopisthocotylea), a gill parasite from Diplodus argenteus (Valenciennes, 1830) The functional morphology of the digestive system has been studied for different Monogenea (Smyth & Halton 1983, Bogitsh 1993 and the feeding in polyopisthocotyleans and monopisthocotyleans is generally distinct with the former ingesting blood (Llewelyn 1954, Halton 1982 and the latter, mucus and epithelial cells. However, blood pigments were observed in a few monopisthocotyleans (Uspenskaya 1962, Kearn 1963, Fournier 1978, Buchmann et al. 1987) while Allen and Tinsley (1989) demonstrated blood and epithelial cells at the same time in the gut lumen of a polyopisthocotylean species.Atriaster heterodus Lebedev & Paruchin, 1969 (Polyopisthocotylea) was recently reported from Rio de Janeiro coast (Santos et al. 1996). Surface topography and ultrastructural aspects of the spermatogenesis of this species have been described by Santos et al. (1997). The ultrastructure of the gastrodermis and the nutrition of this species are described herein. This paper represents the first ultrastructural study of gut caeca and nutrition of a marine monogenean from Brazil. MATERIALS AND METHODSParasites -Sixty five specimens of A. heterodus were obtained from the gills of 25 Diplodus argenteus (Valenciennes, 1830) Sparidae, collected in Copacabana beach, Rio de Janeiro, Brazil.Histochemistry -Monogeneans and gill filaments were fixed in 5% buffered formalin or 70% ethanol. Samples were embedded in paraffin and cut in 5 mM sections which were deparaffinized with xylene and prior to staining, hydrated in decreasing concentrations of ethanol to water. Histochemical reactions are indicated with the references and color of positive reactions as follows. Lilly and Fullmer (1976) were tested Perls (+=blue-green), Okajima (+=or-ange-red) and Dunn-Thompson (+= emerald green). The picric alcohol solubility test (Llewelyn 1954) was tested for hematin (+=solve).Ultrastructure -For ultrastructural studies, parasites were fixed for 2 hr at 4°C in a solution containing 4% paraformaldehyde, 2.5% glutaraldehyde and 0.3M sucrose in 0.1M cacodylate buffer in sea water, pH 7.2. After being washed in the same solution, they were post-fixed for 1 hr in dark conditions in a solution containing 1% OsO 4 , 0.8% potassium ferricyanide, and 5mM CaCl 2 in

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Section: Discussionmentioning

confidence: 49%

Section: Discussionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 86%

mentioning

confidence: 99%

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The Ultrastructure of the Gastrodermis and the Nutrition of the Gill Parasitic Atriaster heterodus Lebedev and Paruchin, 1969 (Platyhelminthes: Monogenea)

Santos

Souto-Padrón

Lanfredi

1998

Mem. Inst. Oswaldo Cruz

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“…Polyopisthocotyleans, to which H. okamotoi belongs, are all blood feeders, and host blood is digested within the cells that are arranged on the surface of the gut of the parasites (Llewellyn, 1954;Halton and Jennings, 1965). They are called hematin cells (Halton et al, 1968), as they accumulate membrane-bound vesicles which contain undigested hematin originating from the host erythrocytes.…”

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confidence: 99%

Early Development of the Monogenean Heterobothrium okamotoi on the Gills of Tiger Puffer Takifugu rubripes

2004

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Ultrastructural observations on the epidermis of the polyopisthocotylinean monogeneans Rajonchocotyle emarginata and Plectanocotyle gurnardi

Lyons

1972

Z. Parasitenk.

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Electron Microscope Studies of Diclidophora merlangi (Monogenea: Polyopisthocotylea). I. Ultrastructure of the Cecal Epithelium

Cited by 29 publications

References 0 publications

The Ultrastructure of the Gastrodermis and the Nutrition of the Gill Parasitic Atriaster heterodus Lebedev and Paruchin, 1969 (Platyhelminthes: Monogenea)

The Ultrastructure of the Gastrodermis and the Nutrition of the Gill Parasitic Atriaster heterodus Lebedev and Paruchin, 1969 (Platyhelminthes: Monogenea)

Early Development of the Monogenean Heterobothrium okamotoi on the Gills of Tiger Puffer Takifugu rubripes

Ultrastructural observations on the epidermis of the polyopisthocotylinean monogeneans Rajonchocotyle emarginata and Plectanocotyle gurnardi

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