Electron Microscope Studies of the Epidermis of Allium Cepa

Scott, Flora Murray; Hamner, K. C.; Baker, Elizabeth; Bowler, Edwin

doi:10.1002/j.1537-2197.1958.tb13150.x

Cited by 57 publications

(22 citation statements)

References 12 publications

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“…The wax layer of sultana grapes, as revealed by the carbon-replica technique and electron microscopy, shows some similarities with the wax layers that occur on the leaf surfaces of the plant species investigated by Mueller, Carr, and Loomis (1954), Scott et al (1958), and Juniper and Bradley (1958). The sultana wax consists oflarge flat platelets rather than rods which are a feature of many species.…”

Section: Discussionmentioning

confidence: 65%

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Studies of the Fine Structure of the Wax Layer of Sultana Grapes

Chambers¹,

Possingham²

1963

Aust. Jnl. Of Bio. Sci.

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The surface waxy bloom of sultana grapes has been studied using the carbon. replica technique in combination with electron microscopy. This layer consists of a series of overlapping wax platelets, each of which is about 0·1 f' wide. The air spaces between the wax platelets become filled with liquid when sultana berries are dipped in commercial emulsions known to accelerate their drying rate. Washing in distilled water removes this layer of dipping emulsion from the surface. The appearance of dipped and washed grapes is similar to that of untreated grapes.Rubbing the grapes with lens tissue destroys the precise arrangement of wax platelets but does not remove the wax. Dipping the grapes in chloroform (four changes of 10 sec), a treatment known to remove most of the wax, leaves sufficient wax to enable replicas to be prepared. Treatment of the grapes with chloroform for longer periods and Soxhlet extraction removes all the waxy structures.These results are discussed, and a hypothesis is presented which may explain the mechanism of action whereby dipping emulsions increase the rate of water movement from grapes.

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Section: Discussionmentioning

confidence: 65%

“…(1954, quoted by van Overbeek 1956) and Scott et al (1958). These workers have shown that the epidermis of leaves consists of an outer layer of wax covering a cuticle, which is composed of a mixture of wax and cutin.…”

Section: Discussionmentioning

confidence: 99%

Studies of the Fine Structure of the Wax Layer of Sultana Grapes

Chambers¹,

Possingham²

1963

Aust. Jnl. Of Bio. Sci.

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“…We are not certain what this layer is. The presence of a cuticle layer on the apical meristem of roots of angiosperms (12) and of root hair of Allium cepa (13) had been described. Since this densely stained layer extends from the apex of the root meristem (cap junction) to cover the epidermis of the root, it is likely that this layer is of cuticular nature.…”

Section: Discussionmentioning

confidence: 98%

Regions of Differential Cell Elongation and Mitosis, and Root Meristem Morphology in Different Tissues of Geotropically Stimulated Maize Root Apices

Shen-Miller¹,

McNitt²,

Wojciechowski³

1978

Plant Physiol.

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We examined cell length, mitosis, and root meristem "cuticle" in different tissues of geostimulated, red light-exposed primary roots of corn (Zea Mays, Wisconsin hybrid 64A x 22R). The examination was done at 15-minute intervals for a period of 240 minutes. Differences in cell elongation between the upper and lower sides were most prominent between 1.5 and 2.5 mm from the root meristem; the outer cortex had the greatest elongation growth, and the upper cells showed a significant increase in length compared to the lower. A differential mitosis was also found, with the lower tissue being greater. We infer that the mitotic activity is indicative of cell division, and this division occurs strictly in the first 1.5 mm of the root meristem. The combined effect of differential cell elongation and cell division results in the localization of the geotropic curvature in the 1.5- to 2.5-mm region from the root meristem. Mitosis that occurs primarily in the cortex and stele were asynchronous; the peak of cortical division preceded that of the stele. Both peaks occurred before the peak of geotropism. A densely stained layer separates the cap from the root meristem. This layer is thinner at the apex of the root meristem. The area of the thin region increased with time and peaked at 180 minutes after geostimulation, which was coincidental with the peak of the geotropic response.

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“…Root hairs of many plants are surrounded by a mucilaginous gel (mucigel), which is thought to be composed of pectic substances (SCOTT et al [J 958], DAWES and BOWLER [1959], CORM"ACK [1962], SCOTT, BYSTROM, and BOWLER [1963], JENNY and GROSSENBACHER [J 963]), although its chemical nature has not yet been definitely established. It shows a weak reaction to staining with ruthenium red ), which supports the suggestion that it is a pectic substance.…”

Section: Pectic Substances Gums and Mudlagesmentioning

confidence: 99%

Soil Components

1975

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Electron Microscope Studies of the Epidermis of Allium Cepa

Cited by 57 publications

References 12 publications

Studies of the Fine Structure of the Wax Layer of Sultana Grapes

Studies of the Fine Structure of the Wax Layer of Sultana Grapes

Regions of Differential Cell Elongation and Mitosis, and Root Meristem Morphology in Different Tissues of Geotropically Stimulated Maize Root Apices

Soil Components

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