Electron Microscope Study of Lens Fibers

Wanko, Theodor; Gavin, Mary Ann

doi:10.1083/jcb.6.1.97

Cited by 95 publications

(24 citation statements)

References 8 publications

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“…A RNase-labile affinity for thiazine dyes at pH values of 4.0 and 6.0 suggests that RNA is a major constituent of terminal bodies. Electron micrographs support this view and also show that, ultrastructurally, the subunits of terminal bodies bear a striking resem blance to the two major components of nucleoli [Wanko and Gavin, 1959;Srinivasan and Iwamoto, 1973;Kuwabara and Imaizumi, 1974]. The smaller spheroid is similar to the fibrillar element of nucleoli, and the larger subunit appears more like the granular component.…”

Section: Discussionsupporting

confidence: 49%

RNA-Containing Cytoplasmic Inclusions at the Termini of Maturing Fibers in the Rat Lens

Worgul¹,

Iwamoto²,

Merriam³

1977

Ophthalmic Res

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Light- and electron-microscopic studies of normal rat lenses revealed the presence of heretofore undescribed structures at the termini of the cortical lens fibers. These ‘terminal bodies’ are composed of two spherical subunits: a large sphere approximately 1.0 µm in diameter, containing a granular component, and a smaller sphere, about 0.5 µm in diameter with a fibrillar lattice organization. Histochemical analyses indicate that RNA is a major component of terminal bodies. The data suggest that these bodies may represent highly ordered condensations of cytoplasmic ribosomes and/or nucleolar material.

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Section: Discussionsupporting

confidence: 49%

RNA-Containing Cytoplasmic Inclusions at the Termini of Maturing Fibers in the Rat Lens

Worgul¹,

Iwamoto²,

Merriam³

1977

Ophthalmic Res

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“…Since the radial strain at the equator is always smaller than elsewhere, always within the elastic range at high spinning speeds, and independent of the presence or absence of the capsule (compare A and C) it would seem that the equator of the cortex must be much more resistant to stress than its poles. A probable reason for this is the number of interlocking fibre junctions at suture lines (Wanko & Gavin, 1961).…”

Section: Lens Elastic Constantsmentioning

confidence: 99%

The elastic constants of the human lens

Fisher¹

1971

The Journal of Physiology

315

239

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SUMMARY1. When the lens is spun around its antero-posterior polar axis in an apparatus designed for the purpose, high speed photography can be used to record its changing profile. By this method a variable radial centrifugal force can be applied to the lens which mimics the pull of the zonule.2. If the lens is not stressed at its centre beyond 100 Nm-2 it behaves as a truly elastic body. When stressed beyond this limit visco-elastic strain is produced at its poles.3. The human lens has isotropic elastic properties at the extremes of life, but at the other times Young's Modulus of Elasticity varies with the direction in which it is measured.4. Young's Modulus of Elasticity of the lens varies with age, polar elasticity and equatorial elasticity, at birth being 0 75 x 103 and 0-85 x 103 Nm2 respectively, while at 63 years of age both are equal to 3 x 103 Nm2. 5. A comparison of Young's Modulus of the young human lens with that of the rabbit and cat shows that the polar elasticity of the lenses of these animals was 5 times greater in the young rabbit, and 21 times greater in the adult cat. Equatorial elasticities of the rabbit and human lens were equal, while in the cat the equatorial elasticity was four times greater.6. A mathematical model showing the lens substance possessing a nucleus of lower isotropic elasticity than that of the isotropic elastic cortex surrounding it, accounts for the difference between polar and equatorial elasticity of the intact adult lens.7. The implications of these findings are discussed in relation to: (i) accommodation and the theological properties of the lens; (ii) possible differences in the physical state of the lenticular proteins in the cortex and nucleus which may account for the senile variations in Young's Modulus of Elasticity in these regions of the lens;(iii) the loss of accommodation due solely to an increase in Young's Modulus of Elasticity of the lens between the ages of 15 and 60. This would amount to 44 % of the total observed in vivo.

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“…The regional differences in cellular fine structures in the old lens regenerate observed in the present stndy (p. 272) are in general agreement with what were described by other authors (COHEN 1958. andGAVIN 1959). Remarkable alterations in the subcellular structures occurred during the early stages of the fiber formation.…”

Section: Alteration Of Subcellutar Components During Lens Fiber Formamentioning

confidence: 99%

“…These elements resemble the basic structure of the lens fiber of the old regenerate (p. 270) and of the lens of other species GAVIN 1959 in adult rabbits, rats, monkeys, andcalves, andRESINK et al 1960 in rabbits andcalves). GAVIN (1959) andRESNIK et al (1960) have demonstrated that those fine elements of low density closely correlate with the clusters of dense granules. KARASAKI (1964) also observed the clusters of ribosomes in the differentiating lens cells.…”

Section: Alteration Of Subcellutar Components During Lens Fiber Formamentioning

confidence: 99%

Electron Microscopic Studies on Lens Regeneration

Eguchi

1964

Embryologia

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Electron Microscope Study of Lens Fibers

Cited by 95 publications

References 8 publications

RNA-Containing Cytoplasmic Inclusions at the Termini of Maturing Fibers in the Rat Lens

RNA-Containing Cytoplasmic Inclusions at the Termini of Maturing Fibers in the Rat Lens

The elastic constants of the human lens

Electron Microscopic Studies on Lens Regeneration

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