Electron Microscopic Cytochemistry of 5-Hydroxytryptamine (5-HT) in the Beta Cells of Guinea Pig Endocrine Pancreas

Jaim-Etcheverry, Guillermo; Zieher, Luis Marı́a

doi:10.1210/endo-83-5-917

Cited by 94 publications

(36 citation statements)

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“…Thus, its effect might be due to a modification at the level of the glucose-membrane receptor, like the one described with alpha-neuraminidase [17]. An other possibility is that the increment of the serotonin within the beta granules [12] could modify the granules availability for emiocytosis. Fig.…”

Section: Discussionmentioning

confidence: 99%

The effect of serotonin on in vitro insulin secretion and biosynthesis in mice

1974

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Summary. The effect of serotonin on insulin secretion and biosynthesis was studied using isolated islets of mice. Serotonin produced a small stimulatory effect on insulin secretion when glucose was present in the incubation medium at a low concentration. On the other hand, an inhibition of insulin secretion was obtained with serotonin when glucose in the medium reached 3.0 mg/ml concentration. No significant effect of serotonin was obtained on insulin biosynthesis, neither in the presence of low nor with a high glucose concentration. These results suggest that the effect of this monoamine on insulin secretion is not mediated via its effect on insulin biosynthesis.

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Section: Discussionmentioning

confidence: 99%

The effect of serotonin on in vitro insulin secretion and biosynthesis in mice

1974

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“…Due to difficulties in making reliable insulin specific electrodes, a more basic serotonin-sensing carbon fiber is often used to measure exocytosis from cells, which have been preincubated with serotonin for 2-24 h [6,7]. Serotonin is taken up into the b-cell, localizes to the insulin secretory granules [8,9] and is then co-secreted with insulin [10]. Serotonin release from single preloaded pancreatic b-cells has been measured using the amperometric technique and has correlated well to insulin exocytosis as measured by the same technique using the specific insulin sensitive carbon fiber electrode [11].…”

Section: Introductionmentioning

confidence: 99%

³H‐serotonin as a marker of oscillatory insulin secretion in clonal β‐cells (INS‐1)

et al. 2007

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Serotonin release from preloaded pancreatic b-cells has been used as a marker for insulin release in studying exocytosis from single cells using the amperometric technique. We found that single pancreatic b-cells exhibited oscillations in exocytosis with a period of 1-1.5 min as measured amperometrically by serotonin release. We also show that 3 H-serotonin can be used to monitor exocytosis from intact and streptolysin-O permeabilized clonal insulin-secreting cells preloaded with labeled serotonin and that serotonin release correlated with insulin secretion in the same cells. The use of 3 H-serotonin provides a real-time indicator of exocytosis from populations of clonal insulin-secreting cells.

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“…11 C]5-HTP will, after decarboxylation, share the fate of endogenous serotonin, including uptake into secretory vesicles by the vesicular monamine transporter 2 (VMAT2) or biodegradation by monoamine oxidase-A (MAO-A) into 5-hydroxyindoleacetic acid (HIAA), which is excreted into urine (15,(18)(19)(20)(21). Radiolabeled 5-HTP therefore accumulates in the form of serotonin in islets of Langerhans as opposed to the exocrine pancreatic parenchyma (9,22).…”

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Positron Emission Tomography Ligand [11C]5-Hydroxy-Tryptophan Can Be Used as a Surrogate Marker for the Human Endocrine Pancreas

et al. 2014

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In humans, a well-developed serotonin system is localized to the pancreatic islets while being absent in exocrine pancreas. Assessment of pancreatic serotonin biosynthesis could therefore be used to estimate the human endocrine pancreas. Proof of concept was tested in a prospective clinical trial by comparisons of type 1 diabetic (T1D) patients, with extensive reduction of β-cells, with healthy volunteers (HVs). C-peptide–negative (i.e., insulin-deficient) T1D subjects (n = 10) and HVs (n = 9) underwent dynamic positron emission tomography with the radiolabeled serotonin precursor [11C]5-hydroxy-tryptophan ([11C]5-HTP). A significant accumulation of [11C]5-HTP was obtained in the pancreas of the HVs, with large interindividual variation. A substantial and highly significant reduction (66%) in the pancreatic uptake of [11C]5-HTP in T1D subjects was observed, and this was most evident in the corpus and caudal regions of the pancreas where β-cells normally are the major constituent of the islets. [11C]5-HTP retention in the pancreas was reduced in T1D compared with nondiabetic subjects. Accumulation of [11C]5-HTP in the pancreas of both HVs and subjects with T1D was in agreement with previously reported morphological observations on the β-cell volume, implying that [11C]5-HTP retention is a useful noninvasive surrogate marker for the human endocrine pancreas.

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Electron Microscopic Cytochemistry of 5-Hydroxytryptamine (5-HT) in the Beta Cells of Guinea Pig Endocrine Pancreas

Cited by 94 publications

References 0 publications

The effect of serotonin on in vitro insulin secretion and biosynthesis in mice

The effect of serotonin on in vitro insulin secretion and biosynthesis in mice

³H‐serotonin as a marker of oscillatory insulin secretion in clonal β‐cells (INS‐1)

Positron Emission Tomography Ligand [11C]5-Hydroxy-Tryptophan Can Be Used as a Surrogate Marker for the Human Endocrine Pancreas

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Electron Microscopic Cytochemistry of 5-Hydroxytryptamine (5-HT) in the Beta Cells of Guinea Pig Endocrine Pancreas

Cited by 94 publications

References 0 publications

The effect of serotonin on in vitro insulin secretion and biosynthesis in mice

The effect of serotonin on in vitro insulin secretion and biosynthesis in mice

3H‐serotonin as a marker of oscillatory insulin secretion in clonal β‐cells (INS‐1)

Positron Emission Tomography Ligand [11C]5-Hydroxy-Tryptophan Can Be Used as a Surrogate Marker for the Human Endocrine Pancreas

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³H‐serotonin as a marker of oscillatory insulin secretion in clonal β‐cells (INS‐1)