Electron Microscopic Demonstration and Isolation of Ribosomes in Mesosomes from <i>Staphylococcus aureus</i>

Nakasone, Noboru; Kawata, Tomio; Masuda, Kuniyoshi

doi:10.1111/j.1348-0421.1987.tb03066.x

Cited by 2 publications

(4 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The purified mesosomal preparation was composed of closed, short tubules and vesicles (Fig. 2a), indicating that fragmentation occurred during the isolation process, since mesosomes were long and branched tubular structures in situ (4,17,27). The mesosomal vesicles and tubules isolated here were not penetrated by the negative stain, indicating that they were packed with contents.…”

Section: Isolation and Morphology Of Mesosomesmentioning

confidence: 95%

“…M-ribosomes have the same sedimentation profile and morphological appearance as C-ribosomes. In S. aureus we previously showed that M-ribosomes could not be distinguished in morphological images as well as in sedimentation profiles from C-ribosomes (27).…”

Section: Discussionmentioning

confidence: 99%

“…The mesosomal vesicles and tubules isolated here were not penetrated by the negative stain, indicating that they were packed with contents. Thin sections of the isolated mesosomes revealed that they contained electron-dense ribosome-like particles, like those observed in Escherichia coli (3,21) and S. aureus (27) upon electron microscopy of negatively stained preparations (Fig. 4).…”

Section: Isolation and Morphology Of Mesosomesmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of Mesosomes of Micrococcus luteus: Isolation and Properties of Mesosomal Ribosomes, and Localization of Penicillin‐Binding Proteins in Mesosomal Membranes

Nakasone¹,

Masuda²,

Kawata³

1987

Microbiology and Immunology

Self Cite

View full text Add to dashboard Cite

Mesosomes were isolated and purified from Micrococcus luteus under hypertonic conditions throughout preparation processes. The purified mesosomal preparation was composed of closed tubules and vesicles. Electron-dense ribosomelike particles were observed within the isolated mesosomal vesicles by electron microscopy. The ribosome-like particles were isolated from the purified mesosomes by a procedure involving solubilization of the membranes with detergents followed by centrifugation on a linear density gradient of sucrose. The isolated particles have a sedimentation coefficient of 70S in the presence of 10 MM Mg2+, when Mg2+ concentration was lowered to 0.1 mM, the particles were dissociated into two sub-particles of 30S and 50S. The 70S particles had the same appearance as cytoplasmic 70S ribosome particles upon observations of negatively stained preparations. These findings indicate that mesosomal tubules contain ribosomes. The isolated mesosomal ribosomes had the ability for protein synthesis when polyuridylic acid-directed polyphenylalanine synthesis was assayed. The sensitivity of mesosomal ribosomes to inhibitors, chloramphenicol and streptomycin, for protein synthesis was significantly lower than that of both cytoplasmic and cytoplasmic membrane-bound ribosomes. In addition, three penicillin-binding proteins were detected in the mesosomal membranes. One of these was localized predominantly in the mesosomal membranes and the other two were distributed almost equally in both mesosomal and cytoplasmic membranes.

show abstract

Section: Isolation and Morphology Of Mesosomesmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Isolation and Morphology Of Mesosomesmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Mesosomes of Micrococcus luteus: Isolation and Properties of Mesosomal Ribosomes, and Localization of Penicillin‐Binding Proteins in Mesosomal Membranes

Nakasone¹,

Masuda²,

Kawata³

1987

Microbiology and Immunology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Due to its aggressiveness and to the existence and proliferation of many multidrug-resistant strains, S. aureus is a major public health concern [13]. To our knowledge, only S. aureus ribosomes and not the polysomes have been studied [4,[14][15][16][17][18].…”

mentioning

confidence: 99%

Purification, identification, and functional analysis of polysomes from the human pathogen Staphylococcus aureus

Brielle

Pinel-Marie

Chat

et al. 2017

Methods

View full text Add to dashboard Cite

To cite this version:Régine Brielle, Marie-Laure Pinel-Marie, Sophie Chat, Reynald Gillet, Brice Felden. Purification, identification, and functional analysis of polysomes from the human pathogen Staphylococcus aureus. Methods, Elsevier, 2017, 117, pp.59-66. 10.1016/j.ymeth.2016 Keywords:Polysome, ribosome, translation, Staphylococcus aureus, sucrose gradient purification, translatome. AbstractPolysomes are macromolecular complexes made up of multiple ribosomes simultaneously translating a single mRNA into polypeptide chains. Together, the cellular mRNAs translated in this way are referred to 'translatome.' Translation determines a cell's overall gene expression profile. Studying translatome leads to a better understanding of the translational machinery and of its complex regulatory pathways. Given its fundamental role in cell homeostasis and division, bacterial translation is an important target for antibiotics. However, there are no detailed protocols for polysome purification from Staphylococcus aureus, the human pathogen responsible for the majority of multi-drug resistance issues. We therefore developed methods for the isolation of active polysomes, ribosomes, and ribosomal subunits, examining the purity and quality of each fraction and monitoring polysomal activity during protein synthesis. These steps are mandatory for the use of purified S. aureus polysomes and ribosomes for structural studies or for genome-scale analysis of most translated mRNAs.

show abstract

Electron Microscopic Demonstration and Isolation of Ribosomes in Mesosomes from Staphylococcus aureus

Abstract: Mesosomes of Staphylococcus aureus 209P were observed

Cited by 2 publications

References 17 publications

Characterization of Mesosomes of Micrococcus luteus: Isolation and Properties of Mesosomal Ribosomes, and Localization of Penicillin‐Binding Proteins in Mesosomal Membranes

Characterization of Mesosomes of Micrococcus luteus: Isolation and Properties of Mesosomal Ribosomes, and Localization of Penicillin‐Binding Proteins in Mesosomal Membranes

Purification, identification, and functional analysis of polysomes from the human pathogen Staphylococcus aureus

Contact Info

Product

Resources

About