Electron-microscopic immunolabelling of vasoactive substances in human umbilical endothelial cells and their actions in early and late pregnancy

Sexton, Anita; Loesch, Andrzej; Turmaine, Mark; Miah, S.; Burnstock, Geoffrey

doi:10.1007/s004410050577

Cited by 10 publications

(7 citation statements)

References 36 publications

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“…Histamine could cause sustained vasoconstriction in all HUV in the present study, consistent with the previous non-IVF related study [ 32 ]. The maximum contractile responses to histamine in HUV were also analogous to previous study [ 32 ].…”

Section: Discussionsupporting

confidence: 93%

In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein

Tang

et al. 2023

Reprod Biol Endocrinol

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Objective In vitro fertilization-embryo transfer (IVF-ET) technologies (especially frozen ET) have been widely used, which might affect maternal and fetal health. Information regarding influence of IVF-ET on the vasoconstriction of human umbilical vein (HUV) is limited. This study determined effects of frozen ET on histamine-mediated vascular responses in HUV and related mechanisms. Methods and results HUVs were collected from frozen ET conceived pregnancy and spontaneously conceived pregnancy (control). Histamine concentration in umbilical plasma was higher in frozen ET group than the control. Histamine-mediated contractile response curve was left-shifted in the frozen ET group when comparing with the control. In isolated HUV rings, H1R showed a critical role in regulating vascular constriction, while H2R played little roles in regulating vessel tone. Iberiotoxin and 4-aminopyridine didn’t significantly change histamine-mediated constriction in HUVs. Histamine-induced vasoconstrictions were significantly decreased by nifedipine, KN93, or GF109203X, while the inhibitory effects were significantly greater in the frozen ET group in comparison to the control. The constrictions by Bay K8644, phenylephrine, or PDBu were stronger in frozen ET, respectively. There was a decrease in the protein expressions of H1R and H2R, an increase in protein expressions of BKCaα and PKCβ. Conclusions Histamine-induced constriction in HUV was mainly via H1R. The increased sensitivity to histamine in HUV following frozen ET cycles were linked to the enhanced PKCβ protein expression and function. The new data and findings in this study provide important insight into influences of frozen ET on fetal vessel development and potential influence in long-term.

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Section: Discussionsupporting

confidence: 93%

In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein

Tang

et al. 2023

Reprod Biol Endocrinol

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“…During pregnancy, HDC‐expressing mast cells were numerous in the umbilical cord and the placenta. Their location is compatible with a role for histamine in the regulation of fetal blood supply (Sexton et al, 1996). Leucocytes and inflammatory agents have also been proposed to have a regulatory role in the events leading to the parturition of the fetus (Thomson et al, 1999).…”

Section: Discussionmentioning

confidence: 74%

Multiple sites of L‐histidine decarboxylase expression in mouse suggest novel developmental functions for histamine

Karlstedt

Nissinen

Michelsen

et al. 2001

Developmental Dynamics

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Histamine mediates many types of physiologic signals in multicellular organisms. To clarify the developmental role of histamine, we have examined the developmental expression of L-histidine decarboxylase (HDC) mRNA and the production of histamine during mouse development. The predominant expression of HDC in mouse development was seen in mast cells. The HDC expression was evident from embryonal day 13 (Ed13) until birth, and the mast cells were seen in most peripheral tissues. Several novel sites with a prominent HDC mRNA expression were revealed. In the brain, the choroid plexus showed HDC expression at Ed14 and the raphe neurons at Ed15. Close to the parturition, at Ed19, the neurons in the tuberomammillary (TM) area and the ventricular neuroepithelia also displayed a clear HDC mRNA expression and histamine immunoreactivity (HA-ir). From Ed14 until birth, the olfactory and nasopharyngeal epithelia showed an intense HDC mRNA expression and HA-ir. In the olfactory epithelia, the olfactory receptor neurons (ORN) were shown to have very prominent histamine immunoreactivity. The bipolar nerve cells in the epithelium extended both to the epithelial surface and into the subepithelial layers to be collected into thick nerve bundles extending caudally toward the olfactory bulbs. Also, in the nasopharynx, an extensive subepithelial network of histamine-immunoreactive nerve fibers were seen. Furthermore, in the peripheral tissues, the degenerating mesonephros (Ed14) and the convoluted tubules in the developing kidneys (Ed15) showed HDC expression, as did the prostate gland (Ed15). In adult mouse brain, the HDC expression resembled the neuronal pattern observed in rat brain. The expression was restricted to the TM area in the ventral hypothalamus, with the main expression in the five TM subgroups called E1-E5. A distinct mouse HDC mRNA expression was also seen in the ependymal wall of the third ventricle, which has not been reported in the rat. The tissue- and cell-specific expression patterns of HDC and histamine presented in this work indicate that histamine could have cell guidance or regulatory roles in development.

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“…In contrast, SP has growth-promoting actions on endothelial cells (Villablanca et al 1994), whereas adenosine, a breakdown product of ATP, stimulates retinal microvascular endothelial cell migration and tube formation (Lutty et al 1998) Human umbilical vessels are unusual in that not all the substances released during shear stress act on receptors on adjacent endothelial cells to influence local vascular tone. Histamine, 5-HT, ATP and ET, when released from umbilical endothelial cells in response to shear stress, produce local vasoconstriction (Sexton et al 1996). However, endothelial cells in human umbilical veins that contain and release NPY and ANP, as well as NO, are inactive locally and it has been suggested that they may have trophic effects on fetal development (Cai et al 1993 a, b ;Salas et al 1995 ;Sexton et al 1995).…”

Section: Long-term Trophic Actions Of Substances Released From Endothmentioning

confidence: 99%

Release of vasoactive substances from endothelial cells by shear stress and purinergic mechanosensory transduction

1999

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The evidence for release of vasoactive substances from endothelial cells in response to shear stress caused by the viscous drag of passing fluids is reviewed and, in particular, its physiological significance both in shortterm regulation of blood vessel tone and in long-term regulation of cell growth, differentiation, proliferation, and cell death in pathophysiological conditions is discussed. A new concept of purinergic mechanosensory transduction, particularly in relation to nociception, is introduced. It is proposed that distension of tubes (including ureter, vagina, salivary and bile ducts, gut) and sacs (including urinary and gall bladders, and lung) leads to release of ATP from the lining epithelium, which then acts on P2X #/$ receptors on subepithelial sensory nerves to convey information to the CNS.

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Electron-microscopic immunolabelling of vasoactive substances in human umbilical endothelial cells and their actions in early and late pregnancy

Cited by 10 publications

References 36 publications

In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein

In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein

Multiple sites of L‐histidine decarboxylase expression in mouse suggest novel developmental functions for histamine

Release of vasoactive substances from endothelial cells by shear stress and purinergic mechanosensory transduction

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