Electron Microscopic Observation of the Transitional Portion in the Human Eccrine Sweat Gland.

Hagiwara, Haruo; Shibasaki, Susumu

doi:10.1679/aohc.57.67

Cited by 3 publications

(4 citation statements)

References 10 publications

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“…Anti-PCNA labelled a few secretory cells and outer cells of the duct, myoepithelial cells were not stained as in other eccrine glands [Morimoto and Saga, 1995]. In contrast to human eccrine glands, we could not find any highly proliferative germinative cells in the transitional portions or the coiled ducts [Hagiwara and Shibasaki, 1994], but the density of positively stained nuclei was higher in these parts if compared to the secretory compartments.…”

Section: Discussioncontrasting

confidence: 69%

Cutaneous Eccrine Glands of the Foot Pads of the Rock Hyrax (<i>Procavia capensis</i>, Hyracoidea, Mammalia)

Stumpf

Welsch

2002

Cells Tissues Organs

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In order to find correlations between skin gland morphology and specific ethological features, the cutaneous glands of the foot pads of Procavia capensis were studied by histological and various histochemical methods and by electron microscopy. In the foot pads, abundant specific eccrine skin glands occur, which consist of coiled tubular secretory portions and coiled ducts. The wall of the secretory part is composed of cuboidal glandular cells and myoepithelial cells. Among the glandular cells two types occur: clear and dark cells. Clear cells have numerous mitochondria and form a basal labyrinth, indicating fluid transport. Dark cells, which stain strongly with periodic acid-Schiff, contain a highly developed perinuclear Golgi apparatus, large amounts of rough endoplasmic reticulum and many secretory granules indicating production of glycoproteins. Cytokeratin (CK) 19 was found in secretory compartments and ducts, CK14 only in duct cells. Single cells of the secretory coils and ducts may be stained with antibodies against antimicrobial peptides. Some glandular cells contain proliferating cell nuclear antigen-positive nuclei especially in the ducts indicating an increased cell proliferation. Terminal transferase (TdT)-mediated d-UTP nick-end labeling-positive nuclei can be detected predominantly in the secretory coils and rarely in the transitional portions between ducts and end pieces. We suppose that proliferating cells migrate from the ducts to the secretory coils. The secretory product of the eccrine cutaneous glands seems to improve the traction between the foot pads of these animals and the steep and smooth rock formations among which they live.

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Section: Discussioncontrasting

confidence: 69%

Cutaneous Eccrine Glands of the Foot Pads of the Rock Hyrax (<i>Procavia capensis</i>, Hyracoidea, Mammalia)

Stumpf

Welsch

2002

Cells Tissues Organs

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“…The transporters shown at the apical membrane of the inner layer and those at the basolateral membrane of the outer layer are those that have been reported in the literature: at the basolateral membrane are the Na ϩ /K ϩ -AT-Pase pump, localized at both cell layers (37), the chloride channel cystic fibrosis transport regulator (CFTR) (41), and a basolateral K ϩ channel (38,42); at the luminal membrane are the CFTR and the epithelial Na ϩ channel (ENaC) (10,38,44,46) and the vacuolar proton pump (V-ATPase) (11,18). As suggested by previous electron microscopy images (13,20), inner (luminal) and outer (peripheral) cell layers are represented as distinct, showing intercellular spaces between cells and gap junctions. This model differs from previous ones by incorporating the finding of the present study, showing the presence of NHE1 at the basolateral membrane of the outer layer (NHE1basal), at the lateral membrane of both cell layers (NHE1later), and at the junction between the inner and outer cell layers (NHE1inter).…”

Section: Chloride Involvement In Ph I Regulationmentioning

confidence: 73%

“…Given reabsorption rates of ϳ1 nl⅐ min Ϫ1 ⅐ mm Ϫ1 for the sweat duct (30) and an external diameter of 40 m (50), and estimating from the kidney the area of intercellular space per cm 2 of duct surface to be 0.004 (5,48), we obtain 3.33 ϫ 10 Ϫ3 cm/s for V. Next, with a value for epithelium thickness of 15 m (50), a tortuosity factor ␣ for the intercellular spaces, and an estimate of 10 Ϫ5 cm 2 /s for D, the EIPA diffusion coefficient, we obtain Jc /Jd ϭ 0.5␣. The intercellular spaces of the sweat duct are very tortuous (13,20), so ␣ and Jc /Jd must be much larger than 1. ing a concentration gradient of EIPA in these spaces. From this, it can be argued that the convective flux is very likely to reduce substantially the EIPA concentration in the intercellular spaces most remote from the basal side and therefore prevent full blockade of the exchangers in these area.…”

Section: Basolateral and Apical Membranes Of The Sweat Ductmentioning

confidence: 99%

“…Indeed, transmission electron microscopy images of the human eccrine sweat duct (13,20) clearly show extensive lateral interdigitations between neighboring cells, in both the inner and outer layers, associated with a well-developed intercellular space. The same is true between the basal membrane of the inner cells and the apical membrane of the outer cells.…”

Section: Lateral and Interlayer Nhe1mentioning

confidence: 99%

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Na⁺/H⁺ exchangers in the human eccrine sweat duct

Granger¹,

Marsolais²,

Burry³

et al. 2003

American Journal of Physiology-Cell Physiology

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Using an anti-NHE1 antibody, we demonstrate the presence of a Na+/H+ exchanger of isoform 1 (NHE1) in the human eccrine sweat duct. A strong staining was observed at the basolateral membrane of the outer cell layer (NHE1basal), at the junction between inner and outer cells layers (NHE1inter), and along the lateral membranes (NHE1later) of all cells of the duct. At the luminal membrane, no staining was demonstrated either for NHE1 or NHE3. To investigate Na+/H+ mediated proton transport, straight sweat duct portions were isolated and perfused in vitro under HCO3-free conditions. In the presence of basolateral 5-ethyl-N-isopropyl amiloride (EIPA), an acidification of 0.29 +/- 0.03 pH units was observed, whereas no effect was observed with luminal EIPA. Bath sodium removal generated a stronger acidification (0.41 +/- 0.09 pH units). Removal of luminal sodium (in the absence or presence of basolateral EIPA), or low luminal chloride, led to an alkalinization, presumably due to a decrease in intracellular sodium, strongly suggesting functional activity of NHE1inter. We therefore conclude that in the sweat duct, NHE1 plays a major role in intracellular pH regulation.

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Adaptive evolution of secretory cell lines in vertebrate skin

et al. 2006

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The embryonic horny layer of vertebrates contains distinctive cell lines that account for the remarkable, diverse secretory performances of their cutaneous apparatuses. Aquatic classes (jawless, cartilaginous and bony fishes) possess single gland cells, scattered among ordinary epidermal cells, that manufacture proteinaceous or mucous substances. This dichotomy is retained in adult amphibians, although the secretory cells in anamnionic tetrapods begin to be arranged in complex glands located in the loose dermis. In the Amniota, intensive keratinisation involving the epidermis results in the loss of the ancestral secretory lines, accompanied by the onset of a novel, lipidproducing gland type, which in mammals is flanked by the exclusive sweat gland apparatus. In this concise up-to-date review, we attempt to phylogenetically narrow the large variety of secretory structures in vertebrate skin into a few basic schemes, in the light of the close relationships between environmental challenges and ecological, ethological as well as physiological roles of the cutaneous apparatus.

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Electron Microscopic Observation of the Transitional Portion in the Human Eccrine Sweat Gland.

Cited by 3 publications

References 10 publications

Cutaneous Eccrine Glands of the Foot Pads of the Rock Hyrax (<i>Procavia capensis</i>, Hyracoidea, Mammalia)

Cutaneous Eccrine Glands of the Foot Pads of the Rock Hyrax (<i>Procavia capensis</i>, Hyracoidea, Mammalia)

Na⁺/H⁺ exchangers in the human eccrine sweat duct

Adaptive evolution of secretory cell lines in vertebrate skin

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Electron Microscopic Observation of the Transitional Portion in the Human Eccrine Sweat Gland.

Cited by 3 publications

References 10 publications

Cutaneous Eccrine Glands of the Foot Pads of the Rock Hyrax (<i>Procavia capensis</i>, Hyracoidea, Mammalia)

Cutaneous Eccrine Glands of the Foot Pads of the Rock Hyrax (<i>Procavia capensis</i>, Hyracoidea, Mammalia)

Na+/H+ exchangers in the human eccrine sweat duct

Adaptive evolution of secretory cell lines in vertebrate skin

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Product

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Na⁺/H⁺ exchangers in the human eccrine sweat duct