Electron microscopic observations on the human membranous labyrinth with particular reference to Ménière's disease

Friedmann, I.; Cawthorne, Terence; McLay, K.; Bird, E. S.

doi:10.1016/s0022-5320(63)80040-4

Cited by 61 publications

(13 citation statements)

References 10 publications

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“…Ultrastructural changes of the utricle such as loss of sensory hairs, vacuolization of sensory cell cytoplasm, accumulation of lipofuscin droplets in sensory and support ing cells and deposition of long-spacing col lagen fibers in the extracellular space related to the basal part of the neuroepithelium have been described in patients with Me niere's disease [6][7][8][9][10][11], However, it was not verified whether these changes were directly related to the disease or represented non specific changes possibly due to age. Thick ening and rounded clumping of the basal lamina in the saccule in a case of endolym phatic hydrops has also been observed light microscopically [12].…”

Section: Discussionmentioning

confidence: 99%

Basal Lamina Pathology of the Utricle and Endolymphatic Duct in Ménière’s Disease

O’Connor

Bagger‐Sjöbäck

Friberg

et al. 1985

ORL

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Labyrinthectomy was performed in a patient with a 4-year history of Ménière’s disease. The utricle and the proximal portion of the endolymphatic duct were studied using light and electron microscopy. The epithelium varied in height from squamöus to columnar. The basal lamina lining the columnar epithelial cells showed marked thickening which was visible in the light microscope. Electron microscopy revealed that the basal lamina had a fibrillar structure. The basal lamina of adjacent blood vessels also showed pathologic thickening with similar fibrillar texture. The significance of basal lamina thickening in association with Ménière’s disease is discussed.

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Section: Discussionmentioning

confidence: 99%

Basal Lamina Pathology of the Utricle and Endolymphatic Duct in Ménière’s Disease

O’Connor

Bagger‐Sjöbäck

Friberg

et al. 1985

ORL

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“…When first described in vestibular hair cells in the 1960s, this structure was thought to be a pathological feature (7,8). It was later found to be a normal component of mammalian vestibular and cochlear inner, but not outer, hair cells (9-11).…”

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confidence: 99%

Striated organelle, a cytoskeletal structure positioned to modulate hair-cell transduction

Vranceanu

Perkins

Terada

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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The striated organelle (SO), a cytoskeletal structure located in the apical region of cochlear and vestibular hair cells, consists of alternating, cross-linked, thick and thin filamentous bundles. In the vestibular periphery, the SO is well developed in both type I and type II hair cells. We studied the 3D structure of the SO with intermediate-voltage electron microscopy and electron microscope tomography. We also used antibodies to α-2 spectrin, one protein component, to trace development of the SO in vestibular hair cells over the first postnatal week. In type I cells, the SO forms an inverted open-ended cone attached to the cell membrane along both its upper and lower circumferences and separated from the cuticular plate by a dense cluster of exceptionally large mitochondria. In addition to contacts with the membrane and adjacent mitochondria, the SO is connected both directly and indirectly, via microtubules, to some stereociliary rootlets. The overall architecture of the apical region in type I hair cells-a striated structure restricting a cluster of large mitochondria between its filaments, the cuticular plate, and plasma membrane-suggests that the SO might serve two functions: to maintain hair-cell shape and to alter transduction by changing the geometry and mechanical properties of hair bundles.actin | cytoskeleton | stereocilia | ultrastructure | sensory receptors H air-cell stereocilia are important for mechanotransduction. Much is known about their structure, their mechanical properties, and their geometric arrangement (1-3). These cells are embedded in the cuticular plate, a dense structure composed of an actin gel (4) located at the apical end of the hair cell, which is thought to act as a rigid platform, helping stereocilia return to their resting positions after stimulus-evoked displacements (5, 6). What has never been investigated is how the cuticular plate might be stabilized by structures underneath it. Do these structures provide a foundation for the cuticular plate and the hair bundle?One such structure is the striated organelle (SO), also known as a laminated (or Friedman's) body, which is a cytoskeletal lattice underlying the apicolateral hair-cell membrane and consisting of alternating thick and thin filament bundles. When first described in vestibular hair cells in the 1960s, this structure was thought to be a pathological feature (7,8). It was later found to be a normal component of mammalian vestibular and cochlear inner, but not outer, hair cells (9-11). A striated structure, similar in position but differing in morphological details, has been described in other vertebrates (12)(13)(14).Slepecky et al. provided a description of the SO (10, 15, 16), including the periodicity of its filaments, their radial direction, attachment to the plasma membrane, and association with microtubules. Previous studies, even those based on conventional transmission electron microscopy (TEM) and deep-etch freeze fracture (9,10,15,17,18), did not provide a picture of the 3D structure of the organelle. Elec...

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“…Surgical biopsy specimens have allowed TEM study of various regions of the inner ear from patients with clinically active disease 19‐21 . Recently, the detailed technique for the TEM study of the entire endolymphatic duct, endolymphatic sac, and vestibular neurosensory epithelia after removal during transmastoid labyrinthectomy has been described 20,21 .…”

Section: Historymentioning

confidence: 99%

Molecular Temporal Bone Pathology: I. Historical Foundation

Wackym

1997

The Laryngoscope

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The application of molecular biology techniques to temporal bone research is resulting in rapid changes in our understanding of the fundamental mechanisms of auditory, facial nerve, and vestibular function. The use of the polymerase chain reaction, cDNA libraries, and in situ hybridization histochemistry, the determination of genetic defects, and the manipulation of transgenic animals are the molecular biology tools that are available to approach these research problems. Knowledge of the molecular pathology that results in the otologic and neuro-otologic dysfunction many of our patients experience is currently in its infancy. A review of the historical foundation of temporal bone pathology and the evolution of the application of cell and molecular biology methods to archival celloidin-embedded human temporal bone material is presented.

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Electron microscopic observations on the human membranous labyrinth with particular reference to Ménière's disease

Cited by 61 publications

References 10 publications

Basal Lamina Pathology of the Utricle and Endolymphatic Duct in Ménière’s Disease

Basal Lamina Pathology of the Utricle and Endolymphatic Duct in Ménière’s Disease

Striated organelle, a cytoskeletal structure positioned to modulate hair-cell transduction

Molecular Temporal Bone Pathology: I. Historical Foundation

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