Electron microscopic study on sporocysts and sporozoites of parental strains and precocious lines of rabbit coccidia Eimeria intestinalis , E. media and E. magna

Pakandl, Michal; Licois, Dominique; Coudert, Pierre

doi:10.1007/s004360000303

Cited by 14 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Each rabbit was inoculated with 1 × 10 2 , 3 × 10 2 , 5 × 10 2 , 1 × 10 3 , 5 × 10 3 Empre oocysts or 5 × 10 3 Emwt oocysts, respectively (N = 4). Fecal samples were collected daily from days 3 to day 10 post inoculation for Empre or daily from days 4.5 to day 12.5 post inoculation for Emwt Previous studies showed that the position, the size, and the number of RB changed when most precocious lines of rabbit coccidia were obtained from their parental strains (Pakandl et al 2001;Pakandl and Jelínkova 2006). In this study, a large RB was seen in each sporocyst of oocyst in Empre, similar to the observation of a previously selected precocious line of E. media (Licois et al 1994).…”

Section: Discussionmentioning

confidence: 99%

Selection and characterization of a precocious line of Eimeria media

Fang

et al. 2019

Parasitol Res

View full text Add to dashboard Cite

Coccidiosis, caused by the infection of Eimeria parasites, is one of the most common diseases in domestic rabbits. Live anticoccidial vaccine formulated with attenuated precocious lines of pathogenic eimerian parasites is expected to be valuable for the control of rabbit coccidiosis as a similar strategy to produce anticoccidial vaccines against chicken coccidiosis has being used for several decades. Eimeria media, moderate pathogenic, is widespread in China. Therefore, attenuated anticoccidial vaccines against rabbit coccidiosis should contain vaccine strain(s) of E. media. In this study, a precocious line of E. media (Empre) was selected by collecting and propagating the early excreted oocysts with 16 successive generations. The prepatent period of Empre reduced from 108 h of its parental strain (Emwt) to 70 h. The fecundity of Empre was about 1/10 to 1/3 lower than that of Emwt. Each sporocyst of Empre sporulated oocyst contained only one large refractile body instead of two smaller ones seen in the parental strain. When vaccinated with 1 × 10 3 or 1 × 10 4 precocious line oocysts, the rabbits were completely protected against homologous challenge with the parental strain 14 days post challenge by terms of body weight gain and oocyst output counting, indicating the efficacy of Empre. Meanwhile, all immunized rabbits showed no clinical sign post immunization, indicating the safety of Empre. For coimmunization, 1 × 10 3 Empre oocysts and 5 × 10 2 oocysts of a precocious line of E. intestinalis (EIP8) were inoculated to each rabbit in a trial. No diarrhea or mortality was found after vaccination, and the weight gains of the vaccinated group were similar to that of unvaccinated-unchallenged control (UUC) group, while the weight gains of the vaccinated group were similar to that of unvaccinated-unchallenged control (UUC) group (P > 0.05), but significantly higher than that of UCC group (P < 0.01) after challenge, indicating it is safe and effective when using co-immunization. These results together show that Empre, as a precocious line, is a good candidate of precocious line of E. media for anticoccidial vaccine development.

show abstract

Section: Discussionmentioning

confidence: 99%

Selection and characterization of a precocious line of Eimeria media

Fang

et al. 2019

Parasitol Res

View full text Add to dashboard Cite

show abstract

“…Eimeria intestinalis and Eimeria magna are pathogenic because they multiply in the intestine, severely damaging the integrity of the intestinal epithelial cells and intestinal villi, and resulting in the increase of toxic substances and the invasion of external bacteria or viruses (Pakandl et al, 2001). Meanwhile, Eimeria infection can cause a series of physiological and biochemical reactions in rabbits, leading to an imbalance of oxidation/antioxidant system and oxidative stress (Allen, 1997).…”

Section: Discussionmentioning

confidence: 99%

Changes of oxidant-antioxidant parameters in small intestines from rabbits infected with E. intestinalis and E. magna

Zhou

Yuan

et al. 2022

World Rabbit Sci.

View full text Add to dashboard Cite

Rabbit coccidiosis is a very serious disease caused by protozoan parasites of the genus Eimeria, which increases the production rate of free radicals, especially reactive oxygen species. When the generation of free radicals exceeds the scavenging capacity of the body’s antioxidant system, the oxidant-antioxidant balance is broken, resulting in oxidative stress. This study was designed to investigate the effect on the oxidant-antioxidant status of rabbits infected with E. intestinalis and E. magna. To this end, eighteen 30-d-old weaned rabbits were randomly allocated into three groups as follows: the E. intestinalis infection group with 3×103 sporulated oocysts of E. intestinalis, the E. magna infection group with 20×103 sporulated oocysts of E. magna, and the uninfected control group. We measured the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) and the contents of malondialdehyde (MDA) in rabbits’ small intestinal tissues (duodenum, jejunum and ileum) of the three groupson day 8. The results showed that CAT activity and MDA levels significantly increased, while the activities of SOD, GSH-Px and T-AOC decreased after E. intestinalis and E. magna infection. Besides, the jejunum and ileum were particularly damaged in the rabbits. It is concluded that the pathological oxidative stress occurs during the E. intestinalis and E. magna infection process and the body’s oxidant-antioxidant balance is disrupted.

show abstract

“…Existem algumas diferenças na obtenção das cepas precoces de coccídeos de galinha e coelho. Diferentemente do observado em Eimeria de galinha, algumas diferenças morfológicas podem ser evidenciadas entre as cepas parentais e precoces de Eimeria de coelho (Pakandl et al, 2001 (Pakandl et al, 2001;Pakandl, 2009). Mesmo apresentando estas diferenças morfológicas, as cepas precoces e parentais de Eimeria de coelho não apresentaram diferenças de padrões de bandas em estudos de RAPD (Ceré et al, 1997).…”

Section: Características Das Espécies De Eimeria De Galinha Domésticaunclassified

Eimeria spp. de coelho e galinha domésticos: desenvolvimento de ensaios moleculares e caracterização filogenética.

Oliveira¹

View full text Add to dashboard Cite

between the evolutionary relationships of different Eimeria species and the feeding habits and habitats of their respective hosts. Finally, the hypothesis of co-evolution of Eimeria parasites and their hosts could not be supported due to the following evidences: (1) there are experimental reports of host-switching events among rodent Eimeria species; (2) the estimated divergence time of Eimeria species is much more recent than the divergence time of their respective vertebrate hosts; and (3) the parasite and host trees cannot be reconciled.

show abstract

Electron microscopic study on sporocysts and sporozoites of parental strains and precocious lines of rabbit coccidia Eimeria intestinalis , E. media and E. magna

Cited by 14 publications

References 6 publications

Selection and characterization of a precocious line of Eimeria media

Selection and characterization of a precocious line of Eimeria media

Changes of oxidant-antioxidant parameters in small intestines from rabbits infected with E. intestinalis and E. magna

Eimeria spp. de coelho e galinha domésticos: desenvolvimento de ensaios moleculares e caracterização filogenética.

Contact Info

Product

Resources

About