Electron Microscopic Study on the Formation of Hardened Molds of Calcium Phosphate Crystals Containing Gelatin

Abstract

Fundamental studies on biocompatible materials were conducted using the mechanism of dentin formation as a model. Hydroxyapatite and fluorinated apatite were synthesized in a gelatin solution. Synthesized apatite was pressed under vacuum, and the mechanical strength was investigated. A hardened mold was prepared by adding amorphous calcium phosphate (ACP). Then, changes in the crystals of the hardened mold dipped in distilled water or 1 ppm fluoride solution were observed with a transmission electron microscope. The results showed that the crystal growth of apatite synthesized in 1% gelatin was suppressed. Moreover, small and homogenous granular apatite was formed in the precipitate synthesized with fluorine. Gelatin contributed to the reduction of brittleness of the hardened mold, and thus a high cutting resistance value was obtained. Furthermore, acceleration of crystal growth and aggregation of fine crystals were observed in the hardened mold when ACP was added to the solution.

Effect of an Amorphous Calcium Phosphate Agent on Artificial Dentin Caries Lesions

“…Furthermore, the hardness, durability, or adhesion to the tooth substance of the ACP + HEPES filler after it had hardened were not investigated. Nevertheless, the aim of this study was to identify a filler with the same components as tooth substance, and since another study has shown that a mixture of calcium phosphate crystals and gelatin, a small molecule derived from collagen, the organic component of dentin, provides sufficient adhesion and hardness 17) , the conditions for carrying out filling on the basis of MI will include adhesive capacity, initial hardening, and shortening of chair time, and further studies will therefore be required.…”