Electron Microscopy in the Diagnosis of BK-Polyoma Virus Infection in the Transplanted Kidney

Herrera, Guillermo A.; Veeramachaneni, Ravindra; Turbat‐Herrera, Elba A.

doi:10.1080/01913120500323399

Cited by 20 publications

(10 citation statements)

References 22 publications

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“…The specific antibody to BK virus is fraud with a high background in tissue sections, making accurate interpretation quite difficult to impossible in some instances. In contrast, the anti SV-40 antibody is much cleaner, has been shown to cross-react with BK virus antigenic determinants, and is, therefore, a better way to diagnose the presence of the BK virus in tissue, although sensitivity may be an issue with this immunohistochemical stain [28]. Studies addressing the differences in sensitivity, if any, between different immunohistochemical stains to detect BK virus (i.e., using specific anti KB virus vs. anti-SV-40 antibodies) have not been performed.…”

Section: Discussionmentioning

confidence: 86%

BK Polyomavirus in Renal Transplants: Role of Electron Microscopy and Immunostaining in Detecting Early Infection

Latif

Zaman

Veeramachaneni

et al. 2007

Ultrastructural Pathology

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Reactivation of BK polyomavirus (BKV) is increasingly recognized as a cause of failure of renal allografts. Since no specific treatment is available for this infection, early diagnosis is important, as it allows for early intervention and possible recovery of renal function. Forty-four consecutive renal transplant biopsies performed over a 2-year period were included in the study. In addition to evaluation of renal biopsy tissue sections using routine histochemical stains, CD3, CD20, BK virus immunostains using the specific BK virus and the SV40 antibodies and electron microscopy studies were performed. None of the transplant cases but one exhibited classical histologic viral changes. Viral particles were seen by EM in 19%, and BK-virus positivity was identified in only 43% of these cases. CD20-rich inflammatory infiltrates predominated in cases in which either positive BK stain and/or viral particles were identified ultrastructurally. A combined approach using electron microscopic and immunohistochemical evaluation can be utilized effectively to identify BK virus-associated nephropathy at an early phase facilitating early clinical intervention.

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Section: Discussionmentioning

confidence: 86%

BK Polyomavirus in Renal Transplants: Role of Electron Microscopy and Immunostaining in Detecting Early Infection

Latif

Zaman

Veeramachaneni

et al. 2007

Ultrastructural Pathology

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“…The diameter was less than the conventional diameter for polyomaviruses (45 nm), but the size of viral particles can vary according to the method of fixation and embedding (14). In addition, previously published electron microscopy findings for BKPyV particles indicated measurements of 30-50 nm (15). Despite the presence of viral particles indicative of polyomavirus, IHC on lung tissue with a primary antibody against simian virus 40, which is known to cross-react with BK and JC polyomaviruses, was negative.…”

Section: Complete Genome Sequencingmentioning

confidence: 85%

Multiorgan WU Polyomavirus Infection in Bone Marrow Transplant Recipient

Siebrasse¹,

Nguyen²,

Willby³

et al. 2016

Emerg. Infect. Dis.

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“…14B) with smudgy chromatin, nuclear atypia, and anisocytosis, progressing to detachment and apoptosis or necrosis of the epithelial cells. 64,65 These histologic changes can be focal and a ''negative'' biopsy does not rule out BKN. 59 The inclusions can be highlighted by immunoperoxidase staining with antibody for the large T antigen of SV40 virus.…”

Section: Viral Infectionsmentioning

confidence: 97%

Renal Pathology in the Pediatric Transplant Patient

Vogler¹,

Wang²,

Brink³

et al. 2007

Advances in Anatomic Pathology

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Renal transplantation is a therapeutic goal for children with advanced chronic kidney disease. There are many causes of renal dysfunction in children with allografts--the transplanted kidney can develop a variety of morphologic alterations leading to dysfunction. Evaluation of the kidney biopsy is one of the best methods of determining the cause of graft dysfunction. Rejection is a major cause of renal allograft failure in children. The morphologic hallmarks of acute antibody-mediated and cell-mediated rejection and chronic allograft nephropathy have been codified in classification strategies that are useful in adults and children. Viral infection and Epstein-Barr virus-driven posttransplant lymphoproliferative disease also occur in the pediatric transplanted kidney. Drug toxicity from immunosuppressive agents also causes characteristic morphologic alterations in the renal allograft. As the survival of pediatric heart and liver transplant patients improves, the incidence of immunosuppression therapy-related disease in the native kidney in these patients will likely become more important clinically. In addition to renal lesions related to the allograft state, glomerular disease can recur or occur de novo in renal allografts. Here, we describe the pathology of the more common morphologic lesions in kidneys of children with a renal allograft.

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Electron Microscopy in the Diagnosis of BK-Polyoma Virus Infection in the Transplanted Kidney

Cited by 20 publications

References 22 publications

BK Polyomavirus in Renal Transplants: Role of Electron Microscopy and Immunostaining in Detecting Early Infection

BK Polyomavirus in Renal Transplants: Role of Electron Microscopy and Immunostaining in Detecting Early Infection

Multiorgan WU Polyomavirus Infection in Bone Marrow Transplant Recipient

Renal Pathology in the Pediatric Transplant Patient

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