Electron Microscopy of Articular Cartilage in the Young Adult Rabbit

Davies, D. A. L.; Barnett, C. H.; Cochrane, W. A.; Palfrey, A. J.

doi:10.1136/ard.21.1.11

Cited by 117 publications

(47 citation statements)

References 18 publications

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“…Transmission and scanning electron microscopic studies of human (18,22,24,(53)(54)(55), guinea pig (26), mouse (23,56,57), and rabbit (17,19) articular cartilage have confirmed this pattern ( Figures 4A, 4B, and 5). I n the immediate vicinity of cells, collagen fibrils sweep in capsular fashion about the lacunae ( Figure 5).…”

Section: Diagram Of the Fibrous Architecture Of Human Articular Cartimentioning

confidence: 55%

“…T h e diameter of the microfibrillar unit in articular cartilage varies with the species, the age of the individual, and the depth of cartilage. In general these collagen fibril diameters vary from 10 nm to over 100 nm (14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26). T h e typical banded pattern associated with collagen microfibrils results from the quarter stagger of the individual collagen molecules.…”

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confidence: 99%

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Review of articular cartilage collagen research

Lane

Weiss

1975

Arthritis & Rheumatism

148

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Recent advances in collagen methodology have now permitted a detailed investigation of this major structural protein of articular cartilage. Collagen structure, metabolism, antl morphology have been extensively studied in "soft" connective tissues (1,Z). Tlie difficulty of collagen extraction from cartilage and bone has prevented its characterization in "hard" connective tissues until recently (3). Since September 3, 1974; accepted May 5, 1975. in the rapidly progressing field of articular cartilage collagen research.Articular cartilage is a highly specialized connective tissue consisting of relatively small numbers of cells distributed throughout abundant extracellular matrix. T h e biophysical properties of articular cartilage that allow joints to serve as bearings in locomotion are in part related to tlie unique composition of tlie extracellular matrix. Collagen constitutes approximately 10% of the wet and 50y0 of the dry weight of cartilage (5). Noncollagenous material constitutes approximately the other 507, of the dry mass of articular cartilage matrix, of which the bulk is in the form of heterogenous proteoglycan complexes (6). Rather than being a single compound, the proteoglycans comprise multiple compounds that differ from each other in their sulfate content, amino acicl sequence of the peptide core, glycosaminoglycan composition, and side chain length.T h e biomaterial properties of the cartilage are a consequence of the interaction of the collagen and proteoglycans in the extracellular matrix. T h e chain extensions of the proteoglycans antl the electrostatic charges provide the molecular basis for consequent mechanical matrix stiffness (7). Tlie inability of the sulfate moieties to diffuse away from the glycosaminoglycan side chains influences the partition of water and diffusible electrolytes (8,9). T h e interaction of the proteoglycan with collagen is necessary for them to behave like gels (7). Whether the interaction is chem-

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Section: Diagram Of the Fibrous Architecture Of Human Articular Cartimentioning

confidence: 55%

mentioning

confidence: 99%

Review of articular cartilage collagen research

Lane

Weiss

1975

Arthritis & Rheumatism

148

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“…According to Davies et al these lipid droplets are a readily accessible store of material which can be used as a source of energy or chemical building blocks for metabolic activities of the cell. 11 Increase in these lipid droplets (osmophilic bodies) in OA could be a sign of degeneration.…”

Section: Resultsmentioning

confidence: 99%

Ultrastructure of Chondrocytes in Osteoarthritic Femoral Articular Cartilage

Goyal¹,

Gupta

Joshi

2015

Kathmandu Univ. Med. J.

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BackgroundOsteoarthritis (OA) is a common problem in elderly, but it is not an inevitable feature of ageing. About 80-90% of individuals of both sexes have radiographic evidence of OA by the time they reach an age of 65. But not all of them have the symptoms like pain and decreased joint motion. ObjectiveThe objective of the present study was conducted to find out whether the osteoarthritic changes in human articular cartilage are similar to the ageing process or not. MethodsFemoral articular cartilage specimens obtained from 13 osteoarthritic patients (52-80years) undergoing total knee replacement and 9 cadavers of same age group (50-80years) (control) were processed and studied under electron microscope. The ultrastructure of the cartilage from the two groups was compared with each other. ResultsUnder the electron microscope, articular cartilage from control group had chondrocytes having a secretary cell characteristic with prominent nucleus and well developed organelles. In osteoarthritic cartilage, degenerating or necrotic chondrocytes were found. Nuclei of these chondrocytes appeared lobulated or indented. Chondrocytes below the fibrillated surface had dilated and irregular endoplasmic reticulum. Electron dense lipid deposits in the extracelluar matrix as well as intracytoplasmic glycogen deposits were much increased in osteoarthritic cartilage as compared to the control group. Amount of perinuclear intracytoplasmic fine filaments was also increased in the chondrocytes of osteoarthritic cartilage. ConclusionUltrastructural findings of the osteoarthritic articular cartilage were much different from the ageing non-osteoarthritic cartilage. Hence, OA should be considered a specific process and not simply an inevitable feature of ageing.

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“…Les premières études faites au microscope électronique à transmission confirmaient les résultats antérieurs. D'après Davies [8] en 1962 la rugosité est inférieure à 0,025 /lm et d'après Weiss [9] en 1968 les dépressions observées seraient inférieures à 0,3 /lm. Le trop faible grossissement obtenu à l'aide d'un microscope à transmission a amené l'utilisation du microscope à balayage.…”

Section: Description Et Fonctionnement De L'articulationunclassified

Les propriétés mécaniques des articulations et les aspects phisyques de la lubrification

Renaudeaux

1978

La Houille Blanche

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On estime que 50 000 prothèses de hanche seront posées en France cette année et que ce nombre est en augmentation rapide. Environ 1000 opérés devront à nouveau subir une intervention à la suite de défaillances mécaniques des prothèses.Ces deux statistiques montrent que la compréhension de la mécanique articulaire est un problème d'actualité. Description et fonctionnement de l'articulationLes grandes articulations humaines (hanche, genou, etc.) ont évidemment des formes géométriques diffé-rentes. Elles sont cependant constituées suivant le même modèle ( Fig. 1) et comprennent les 3 éléments importants pour la lubrification: os, cartilage, synovie.

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Electron Microscopy of Articular Cartilage in the Young Adult Rabbit

Cited by 117 publications

References 18 publications

Review of articular cartilage collagen research

Review of articular cartilage collagen research

Ultrastructure of Chondrocytes in Osteoarthritic Femoral Articular Cartilage

Les propriétés mécaniques des articulations et les aspects phisyques de la lubrification

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