Electron microscopy of HeLa cells infected with agents of the adenovirus (APC-RI-ARD) group

Lagermalm, Gösta; Kjellén, Lars; Thorsson, K. G.; Svedmyr, Arne

doi:10.1007/bf01240877

Cited by 33 publications

(11 citation statements)

References 14 publications

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“…The size of the virus particle reported here agrees well with all previous findings (1)(2)(3)(4)(5) in which material has been examined without air drying and possible accompanying distortion, and the diameter of the inner body or nucleoid also shows good agreement. The apparent structural differentiation observed here in the nucleoid has not previously been described; although so far no information is available concerning the significance of this finding, it is tempting to regard it as a reflection of the presence of more than one component within this region of the viru~ FIc.…”

Section: Discussionsupporting

confidence: 90%

“…Since the first observation with the electron microscope of intranuclear virus-like particles in sections of tissue culture cells infected with adenovirus (1), numerous later studies of similar material have established certain morphological features of this agent (2)(3)(4)(5). The particles have been reported to be round or ellipsoid and an inner body and outer limiting membrane have been described (3)(4)(5).…”

mentioning

confidence: 99%

“…The particles have been reported to be round or ellipsoid and an inner body and outer limiting membrane have been described (3)(4)(5). On the other hand particles dried from suspension and examined as whole mounts appeared polyhedral in shape (6,7), but some workers consider that this may merely represent an artefact caused by the drying (5).…”

mentioning

confidence: 99%

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Observations on the Fine Structure of Type 5 Adenovirus

Epstein

1959

The Journal of Cell Biology

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Section: Discussionsupporting

confidence: 90%

mentioning

confidence: 99%

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Observations on the Fine Structure of Type 5 Adenovirus

Epstein

1959

The Journal of Cell Biology

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“…of herpes simplex virus was found to crystallize (4) in a manner analogous to the adenoviruses (5)(6)(7)(8)(9). Moreover, the nuclear membranes of cells infected by this strain frequently showed remarkable proliferation.…”

Section: I~a~s 43 To 61mentioning

confidence: 99%

Electron Microscopic Observations on the Development of Herpes Simplex Virus

Morgan

Rose

Holden

et al. 1959

The Journal of Experimental Medicine

262

108

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Electron microscopic study of the H.R. strain of herpes simplex virus in cells of the chicken chorioallantoic membrane (1) suggested that the virus differentiates within the nucleus, where particles possessing a central core and a single limiting membrane are randomly dispersed. In this earlier work the finding that ruptured nuclei were associated with large numbers of intracytoplasmic particles possessing double membranes appeared to be consistent with the hypothesis that the virus acquired a second membrane in the cytoplasm after release from the nucleus. However, cells in tissue culture infected with the H F E M strain of herpes simplex virus and examined by Stoker, Smith, and Ross (2) and herpes B virus examined by Reissig and Melnick (3) subsequently revealed intranuclear particles with double as well as single membranes, thus raising the possibility that development could be completed in the nucleus.Recently, a strain (J.M.) of herpes simplex virus was found to crystallize (4) in a manner analogous to the adenoviruses (5-9). Moreover, the nuclear membranes of cells infected by this strain frequently showed remarkable proliferation. The purposes of this paper are to illustrate and describe the viral crystals as well as the morphology of the cellular response to infection, and to propose an hypothesis concerning the manner of development and the mechanisms whereby virus may gain egress from intact cells. Materials and MetkodsHeLa cells were cultured in a medium consisting of Earle's balanced salt solution which contained 0.5 per cent lactalbumin hydrolysate, 0.25 per cent glucose, 0.1 per cent yeast extract, 0.3 per cent tris(hydroxymethyl)-aminomethane and 20 per cent horse serum. Stable human amnionic cells were cultivated in Eagle's medium to which 20 per cent horse serum * These studies were

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“…However, despite the ubiquity of HeLa ceils in many types of laboratory, relatively little has been reported concerning their fine structure under normal conditions of culture, the electron microscope having mainly been used to study them after infection with various viruses (8)(9)(10)(11)(12)(13). Uninfected HeLa cells are known to contain dense cytoplasmic bodies (14), and the connection of these with ingestion by pinocytosis has been explored using particles of colloidal gold (15).…”

Section: Introductionmentioning

confidence: 99%

SOME UNUSUAL FEATURES OF FINE STRUCTURE OBSERVED IN HELA CELLS

Epstein

1961

The Journal of Cell Biology

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HeLa cells from conventional culture media have been studied in thin sections with the electron microscope; in many cases cells were examined in sets of sections cut in series. The fine structure of the cells is described including three unusual features not hitherto reported. It has been found that numerous cells contained rows of parallel smooth surfaced cisternae spaced about 150 m# apart and communicating with rough surfaced elements of the endoplasrnic reticulum. These cisternae resembled "annulate lamellae" but did not contain regular arrays of pores. In many cells an area of juxtanuclear cytoplasm was occupied by a membranous structure composed of closely applied pairs of narrow cisternae either arranged in concentric rings or else extending in several directions in a haphazard manner. Sparse particles were present on the outer membranes of each pair of cisternae. Communications between the double cisternae and other membrane-bounded structures were not observed. A small number of cells contained areas of cytoplasm devoid of organelles and filled with amorphous fuzzy material. The observations recorded are discussed.

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Electron microscopy of HeLa cells infected with agents of the adenovirus (APC-RI-ARD) group

Cited by 33 publications

References 14 publications

Observations on the Fine Structure of Type 5 Adenovirus

Observations on the Fine Structure of Type 5 Adenovirus

Electron Microscopic Observations on the Development of Herpes Simplex Virus

SOME UNUSUAL FEATURES OF FINE STRUCTURE OBSERVED IN HELA CELLS

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